Cordyceps Polysaccharides Attenuate Experimental Ischemic Stroke Injury: Association with IL-17/NF-κB Signaling
- 1School of Traditional Chinese Medicine, Beijing University of Chinese Medicine, Beijing 102488, China
- 2School of Chinese Materia Medica, Beijing University of Chinese Medicine, Beijing 102488, China
Abstract
Cordyceps polysaccharides (CSP) have shown protective potential in ischemic stroke, but their central inflammatory mechanisms remain unclear. This study examined whether the effects of CSP are associated with interleukin-17 (IL-17)/nuclear factor kappa B (NF-κB)-related signaling. Publicly available human transcriptomic datasets were integrated and analyzed to identify inflammation-related pathways in ischemic stroke, and a public mouse middle cerebral artery occlusion (MCAO) single-cell RNA sequencing (RNA-seq) dataset was analyzed to identify relevant brain cell populations. IL-17 receptor A (IL-17RA) knockdown was performed in oxygen–glucose deprivation (OGD)-exposed SH-SY5Y cells. The effects of CSP were further evaluated in MCAO rats and OGD-exposed BV-2 cells. Bioinformatics analyses showed enrichment of IL-17 and NF-κB signaling in ischemic stroke datasets, and single-cell module-score analysis showed that microglia exhibited the largest MCAO-associated increase in IL-17/NF-κB-related module scores. IL-17RA knockdown improved the viability of OGD-exposed SH-SY5Y cells. In MCAO rats, CSP improved neurological and behavioral outcomes, increased cerebral blood flow, and reduced cerebral edema, infarct volume, neuronal loss, microglial responses, and IL-17A/IL-17RA expression. In OGD-exposed BV-2 cells, CSP reduced IL-17A, IL-17RA, NF-κB activator 1 (ACT1), tumor necrosis factor receptor-associated factor 6 (TRAF6), IL-1β, IL-6, and the phosphorylated p65/total p65 ratio. These findings suggest that the protective effects of CSP in ischemic stroke models are associated with attenuation of IL-17/NF-κB-related neuroinflammation.
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BibTeX
@article{wang2026cordyceps,
title = {{Cordyceps Polysaccharides Attenuate Experimental Ischemic Stroke Injury: Association with IL-17/NF-κB Signaling}},
author = {Huizhang Wang and Yifan Chen and Yang Jiang and Yan Liao and Saiwen Xi and Xia Li and Huiming Qi and Yihui Ma and Yutong Li and Mujie Fang and Changhua Shi and Cong Gai and Yibo Tang},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27209035},
url = {https://www.mdpi.com/1422-0067/27/20/9035},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Cordyceps Polysaccharides Attenuate Experimental Ischemic Stroke Injury: Association with IL-17/NF-κB Signaling
AU - Huizhang Wang
AU - Yifan Chen
AU - Yang Jiang
AU - Yan Liao
AU - Saiwen Xi
AU - Xia Li
AU - Huiming Qi
AU - Yihui Ma
AU - Yutong Li
AU - Mujie Fang
AU - Changhua Shi
AU - Cong Gai
AU - Yibo Tang
PY - 2026
DA - 2026/10/01
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27209035
UR - https://www.mdpi.com/1422-0067/27/20/9035
AB - Cordyceps polysaccharides (CSP) have shown protective potential in ischemic stroke, but their central inflammatory mechanisms remain unclear. This study examined whether the effects of CSP are associated with interleukin-17 (IL-17)/nuclear factor kappa B (NF-κB)-related signaling. Publicly available human transcriptomic datasets were integrated and analyzed to identify inflammation-related pathways in ischemic stroke, and a public mouse middle cerebral artery occlusion (MCAO) single-cell RNA sequencing (RNA-seq) dataset was analyzed to identify relevant brain cell populations. IL-17 receptor A (IL-17RA) knockdown was performed in oxygen–glucose deprivation (OGD)-exposed SH-SY5Y cells. The effects of CSP were further evaluated in MCAO rats and OGD-exposed BV-2 cells. Bioinformatics analyses showed enrichment of IL-17 and NF-κB signaling in ischemic stroke datasets, and single-cell module-score analysis showed that microglia exhibited the largest MCAO-associated increase in IL-17/NF-κB-related module scores. IL-17RA knockdown improved the viability of OGD-exposed SH-SY5Y cells. In MCAO rats, CSP improved neurological and behavioral outcomes, increased cerebral blood flow, and reduced cerebral edema, infarct volume, neuronal loss, microglial responses, and IL-17A/IL-17RA expression. In OGD-exposed BV-2 cells, CSP reduced IL-17A, IL-17RA, NF-κB activator 1 (ACT1), tumor necrosis factor receptor-associated factor 6 (TRAF6), IL-1β, IL-6, and the phosphorylated p65/total p65 ratio. These findings suggest that the protective effects of CSP in ischemic stroke models are associated with attenuation of IL-17/NF-κB-related neuroinflammation.
SN - 1422-0067
ER -