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Peer-reviewedCC BY 4.0Biotechnology Journal

Liquid‐Suspendable Metabolic Sensors Allow Real‐Time Oxygen Consumption Monitoring in Caco‐2 and Colon Organoid‐Based Gut‐on‐a‐Chip Models

  • Sarah Devriese1
  • Flavio Bonanini2
  • Anna F. Bekebrede1
  • Sara Shafiei2
  • Dorota Kurek2
  • Coen Govers3
  • Hans Bouwmeester4
  • A. Paul Vos5
  • Remko van Vught2
  • Jaap Keijer1
  • Vincent C. J. de Boer1
5 affiliations
  1. 1Human and Animal Physiology Wageningen University Wageningen The Netherlands
  2. 2Mimetas B.V. Oegstgeest The Netherlands
  3. 3Cell Biology and Immunology Wageningen University Wageningen The Netherlands
  4. 4Division of Toxicology Wageningen University Wageningen The Netherlands
  5. 5Department of Food, Health and Consumer Research Wageningen Food & Biobased Research Wageningen The Netherlands
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Abstract

The organ‐on‐a‐chip field is a rapidly evolving area of expertise, combining enhanced physiological relevance with a wide range of functional readouts across diverse cellular models. One such readout is oxygen metabolism, which serves as a key indicator of mitochondrial function. In this study, we utilized metabolic sensors designed for real‐time measurement of oxygen consumption, reflecting aerobic respiration, within a commercially available microfluidic platform. We demonstrated that time‐resolved detection of oxygen consumption is feasible within the system's microliter‐scale volumes and that chemically induced changes in cellular oxygen metabolism can be monitored in real time in two ready‐to‐use gut‐on‐a‐chip models. In summary, this novel application of metabolic sensors enables direct evaluation of mitochondrial function in complex cellular environments by addressing key analytical challenges often encountered in organ‐on‐a‐chip systems due to their limited size and structural complexity.

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Cite

BibTeXRIS
BibTeX
@article{devriese2026liquidsuspendable,
  title = {{Liquid‐Suspendable Metabolic Sensors Allow Real‐Time Oxygen Consumption Monitoring in Caco‐2 and Colon Organoid‐Based Gut‐on‐a‐Chip Models}},
  author = {Sarah Devriese and Flavio Bonanini and Anna F. Bekebrede and Sara Shafiei and Dorota Kurek and Coen Govers and Hans Bouwmeester and A. Paul Vos and Remko van Vught and Jaap Keijer and Vincent C. J. de Boer},
  journal = {Biotechnology Journal},
  year = {2026},
  month = nov,
  doi = {10.1002/biot.70333},
  url = {https://doi.org/10.1002/biot.70333},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Liquid‐Suspendable Metabolic Sensors Allow Real‐Time Oxygen Consumption Monitoring in Caco‐2 and Colon Organoid‐Based Gut‐on‐a‐Chip Models
AU  - Sarah Devriese
AU  - Flavio Bonanini
AU  - Anna F. Bekebrede
AU  - Sara Shafiei
AU  - Dorota Kurek
AU  - Coen Govers
AU  - Hans Bouwmeester
AU  - A. Paul Vos
AU  - Remko van Vught
AU  - Jaap Keijer
AU  - Vincent C. J. de Boer
PY  - 2026
DA  - 2026/11/04
JO  - Biotechnology Journal
DO  - 10.1002/biot.70333
UR  - https://doi.org/10.1002/biot.70333
AB  - The organ‐on‐a‐chip field is a rapidly evolving area of expertise, combining enhanced physiological relevance with a wide range of functional readouts across diverse cellular models. One such readout is oxygen metabolism, which serves as a key indicator of mitochondrial function. In this study, we utilized metabolic sensors designed for real‐time measurement of oxygen consumption, reflecting aerobic respiration, within a commercially available microfluidic platform. We demonstrated that time‐resolved detection of oxygen consumption is feasible within the system's microliter‐scale volumes and that chemically induced changes in cellular oxygen metabolism can be monitored in real time in two ready‐to‐use gut‐on‐a‐chip models. In summary, this novel application of metabolic sensors enables direct evaluation of mitochondrial function in complex cellular environments by addressing key analytical challenges often encountered in organ‐on‐a‐chip systems due to their limited size and structural complexity.
SN  - 1860-6768
SN  - 1860-7314
ER  -