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Peer-reviewedCC BYMicroPublication biology

Tools for in vivo labelling of ALFA-tagged proteins in C. elegans .

  • Irini Topalidou1
  • Nicolas Lehrbach1
  1. 1Basic Sciences Division, Fred Hutchinson Cancer Center, Seattle, Washington, United States.
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Abstract

Understanding protein function requires tools to visualize and manipulate proteins in vivo . NanoTags, short epitope tags that are recognized by single-chain antibodies, are an emerging method for versatile in vivo labelling and manipulation of endogenous proteins. The short (13 amino acid) ALFA tag has been shown to allow labelling of endogenous proteins in several model systems, including C. elegans . Here we describe the generation and validation of transgenes to facilitate global and tissue-specific visualization of ALFA-tagged endogenous proteins in C. elegans . We propose a modular NanoTag system as a powerful approach for investigating protein functions in vivo in C. elegans .

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Cite

BibTeXRIS
BibTeX
@article{topalidou2026tools,
  title = {{Tools for in vivo labelling of ALFA-tagged proteins in C. elegans .}},
  author = {Irini Topalidou and Nicolas Lehrbach},
  journal = {MicroPublication biology},
  year = {2026},
  month = oct,
  doi = {10.17912/micropub.biology.002419},
  url = {https://europepmc.org/article/MED/42997131},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Tools for in vivo labelling of ALFA-tagged proteins in C. elegans .
AU  - Irini Topalidou
AU  - Nicolas Lehrbach
PY  - 2026
DA  - 2026/10/21
JO  - MicroPublication biology
DO  - 10.17912/micropub.biology.002419
UR  - https://europepmc.org/article/MED/42997131
AB  - Understanding protein function requires tools to visualize and manipulate proteins in vivo . NanoTags, short epitope tags that are recognized by single-chain antibodies, are an emerging method for versatile in vivo labelling and manipulation of endogenous proteins. The short (13 amino acid) ALFA tag has been shown to allow labelling of endogenous proteins in several model systems, including C. elegans . Here we describe the generation and validation of transgenes to facilitate global and tissue-specific visualization of ALFA-tagged endogenous proteins in C. elegans . We propose a modular NanoTag system as a powerful approach for investigating protein functions in vivo in C. elegans .
SN  - 2578-9430
ER  -