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Peer-reviewedCC BYMicroPublication biology

Use of photostable green fluorescent protein mStayGold for live-cell imaging in Dictyostelium discoideum.

  • Hidenori Hashimura1
  • Shoko Fujishiro1
  • Toyoko Sugita1
  • Hibiki Nakagawa1
  • Satoshi Sawai1,2
  1. 1Graduate School of Arts and Sciences, The University of Tokyo, Meguro-ku, Tokyo, Japan.
  2. 2Research Center for Complex Systems Biology, Universal Biology Institute, The University of Tokyo, Meguro-ku, Tokyo, Japan.
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Abstract

Dictyostelium discoideum is a key Amoebozoa model organism in cell biology, offering unique insights into chemotaxis, cell polarity and the transition from unicellular to multicellular life. Here, we evaluated the highly photostable fluorescent protein mStayGold for live-cell imaging in D. discoideum . mStayGold showed localization patterns comparable to Achilles when fused to Lifeact or the PKBR1(N150) membrane marker. Compared with Achilles, mStayGold exhibited greater resistance to photobleaching under continuous illumination and lower cell-to-cell variability in fluorescence intensity. These properties make mStayGold a promising probe for demanding imaging applications, including super-resolution microscopy, high-speed imaging, long-term developmental imaging and single-molecule tracking.

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Cite

BibTeXRIS
BibTeX
@article{hashimura2026photostable,
  title = {{Use of photostable green fluorescent protein mStayGold for live-cell imaging in Dictyostelium discoideum.}},
  author = {Hidenori Hashimura and Shoko Fujishiro and Toyoko Sugita and Hibiki Nakagawa and Satoshi Sawai},
  journal = {MicroPublication biology},
  year = {2026},
  month = oct,
  doi = {10.17912/micropub.biology.002300},
  url = {https://europepmc.org/article/MED/42953504},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Use of photostable green fluorescent protein mStayGold for live-cell imaging in Dictyostelium discoideum.
AU  - Hidenori Hashimura
AU  - Shoko Fujishiro
AU  - Toyoko Sugita
AU  - Hibiki Nakagawa
AU  - Satoshi Sawai
PY  - 2026
DA  - 2026/10/13
JO  - MicroPublication biology
DO  - 10.17912/micropub.biology.002300
UR  - https://europepmc.org/article/MED/42953504
AB  - Dictyostelium discoideum is a key Amoebozoa model organism in cell biology, offering unique insights into chemotaxis, cell polarity and the transition from unicellular to multicellular life. Here, we evaluated the highly photostable fluorescent protein mStayGold for live-cell imaging in D. discoideum . mStayGold showed localization patterns comparable to Achilles when fused to Lifeact or the PKBR1(N150) membrane marker. Compared with Achilles, mStayGold exhibited greater resistance to photobleaching under continuous illumination and lower cell-to-cell variability in fluorescence intensity. These properties make mStayGold a promising probe for demanding imaging applications, including super-resolution microscopy, high-speed imaging, long-term developmental imaging and single-molecule tracking.
SN  - 2578-9430
ER  -