Molecular Mechanisms Implicated in Myogenic Differentiation of Human Alveolar Mucosa-Derived Cells.
14 affiliations
- 1Institute of Personalized Oncology, I.M. Sechenov First Moscow State Medical University, Moscow 119991, Russia.
- 2Omicsway Corp., 340 S Lemon Ave, 6040, Walnut, CA 91789, USA.
- 3Oncobox Ltd., Moscow 121205, Russia.
- 4Sirius University of Science and Technology, 1 Olimpiyskiy Ave, Sirius 354340, Krasnodar Region, Russia.
- 5Petrovsky National Research Center of Surgery, Moscow 119991, Russia.
- 6Pirogov National Medical and Surgical Center, Moscow 105203, Russia.
- 7Institute for Regenerative Medicine, I.M. Sechenov First Moscow State Medical University, Moscow 119991, Russia.
- 8Artgen Biotech, Moscow 119333, Russia.
- 9Skincell LLC, Moscow 119333, Russia.
- 10Department of Pharmacology, I.M. Sechenov First Moscow State Medical University, Moscow 119991, Russia.
- 11Department of Pharmacognosy and Industrial Pharmacy, Lomonosov Moscow State University, Moscow 119992, Russia.
- 12Department of Pathology, Kazan State Medical University, Kazan 420012, Russia.
- 13Department of Radiotherapy and Radiology, Russian Medical Academy of Continuous Professional Education, Moscow 119454, Russia.
- 14N.N. Blokhin National Medical Research Center of Oncology, Moscow 115522, Russia.
Abstract
Different approaches to skeletal muscle regeneration using progenitor cells are extensively studied as strategies for the treatment of muscle tissue pathologies. Recently, it was discovered that anatomically localized alveolar mucosa multipotent mesenchymal stromal cells (AMCs) are characterized by myogenic potential and high feasibility for muscle tissue recovery and regeneration. Although the resulting multinuclear myotubes express skeletal muscle-specific markers (skeletal myosin, actin, myogenin, and MyoD1), the exact molecular mechanism controlling myogenic differentiation of AMCs is still unclear and poorly scrutinized. In this research, we used a combination of large-scale transcriptome analysis and a bioinformatics approach to investigate molecular pathways and crucial nodes responsible for myogenic differentiation. We studied differentiation of AMC cells in 2D and 3D culture conditions and found core genes with significant expression changes during differentiation and compared them with skeletal muscle-derived stromal cells (SMCs). It appeared that differentiation of AMCs in 3D is significantly different from that in 2D. Moreover, differentiation of AMCs in 2D is closer to differentiation of SMCs in 2D than to AMCs in 3D. Unique properties of AMC differentiation in 3D may be attributed to a significant inhibition of the component of the PDGFRβ signaling pathway responsible for ruffle organization.
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BibTeX
@article{sorokin2026molecular,
title = {{Molecular Mechanisms Implicated in Myogenic Differentiation of Human Alveolar Mucosa-Derived Cells.}},
author = {Maksim Sorokin and Anton Buzdin and Anastasia Guryanova and Alexander Gudkov and Alexander Modestov and Maria Suntsova and Ilya Eremin and Andrey Pulin and Nastasia Kosheleva and Alla Zorina and Vadim Zorin and Dmitry Kudlay and Sergei Boichuk and Maria Vasileva and Maria Fedorova and Svetlana Vinokurova and Pavel Kopnin},
journal = {International journal of molecular sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27198712},
url = {https://europepmc.org/article/MED/42883956},
note = {CC BY},
}
RIS
TY - JOUR
TI - Molecular Mechanisms Implicated in Myogenic Differentiation of Human Alveolar Mucosa-Derived Cells.
AU - Maksim Sorokin
AU - Anton Buzdin
AU - Anastasia Guryanova
AU - Alexander Gudkov
AU - Alexander Modestov
AU - Maria Suntsova
AU - Ilya Eremin
AU - Andrey Pulin
AU - Nastasia Kosheleva
AU - Alla Zorina
AU - Vadim Zorin
AU - Dmitry Kudlay
AU - Sergei Boichuk
AU - Maria Vasileva
AU - Maria Fedorova
AU - Svetlana Vinokurova
AU - Pavel Kopnin
PY - 2026
DA - 2026/09/29
JO - International journal of molecular sciences
DO - 10.3390/ijms27198712
UR - https://europepmc.org/article/MED/42883956
AB - Different approaches to skeletal muscle regeneration using progenitor cells are extensively studied as strategies for the treatment of muscle tissue pathologies. Recently, it was discovered that anatomically localized alveolar mucosa multipotent mesenchymal stromal cells (AMCs) are characterized by myogenic potential and high feasibility for muscle tissue recovery and regeneration. Although the resulting multinuclear myotubes express skeletal muscle-specific markers (skeletal myosin, actin, myogenin, and MyoD1), the exact molecular mechanism controlling myogenic differentiation of AMCs is still unclear and poorly scrutinized. In this research, we used a combination of large-scale transcriptome analysis and a bioinformatics approach to investigate molecular pathways and crucial nodes responsible for myogenic differentiation. We studied differentiation of AMC cells in 2D and 3D culture conditions and found core genes with significant expression changes during differentiation and compared them with skeletal muscle-derived stromal cells (SMCs). It appeared that differentiation of AMCs in 3D is significantly different from that in 2D. Moreover, differentiation of AMCs in 2D is closer to differentiation of SMCs in 2D than to AMCs in 3D. Unique properties of AMC differentiation in 3D may be attributed to a significant inhibition of the component of the PDGFRβ signaling pathway responsible for ruffle organization.
SN - 1422-0067
ER -