Skip to content
DraconiticResearch
Search Sources API
201,818 studies31 sourcesUpdated 31 min ago
Peer-reviewedCC BY 4.0BMC Biotechnology

Isolation and in vitro culture of endothelial progenitor cell-like populations from leukoreduction filters

  • Zahra Abbasi-Malati1,2
  • Seyed Ghader Azizi3
  • Sahar Balagholi1
  • Ali Akbar Pourfathollah4
4 affiliations
  1. 1Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran
  2. 2Department of Tissue Engineering and Applied Cell Sciences, School of Paramedicine, Guilan University of Medical Sciences, Rasht, Iran
  3. 3Clinical Immunology Research Center, Zahedan University of Medical Sciences, Zahedan, Iran
  4. 4Department of Immunology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, 14115-111 Iran
Read full textDOICite

Abstract

Background: Leukoreduction filters (LRFs) are routinely used in blood transfusion services and retain substantial numbers of nucleated cells during blood processing. These discarded filters have therefore been explored as a potential source of peripheral blood-derived cells for endothelial progenitor cell (EPC)-like related in vitro studies.

Methods: Leukoreduction filters were collected from the Iranian Blood Transfusion Organization (IBTO). Entrapped cells were recovered using a washing-based procedure without the use of magnetic-activated cell sorting (MACS) or endothelial differentiation media. A coating solution containing plasma-derived components, including cryoprecipitate and platelet lysate, was applied to improve early cell adhesion during in vitro culture.

Results: Cultured cells were characterized by flow cytometry using CD133 as a surface-associated progenitor marker. Approximately 70% of the cells at passage 3–4 was positive for CD133.

Conclusion: Leukoreduction filters may serve as an accessible and cost-effective source of peripheral blood-derived cell populations for EPC-like related in vitro studies. Further studies are required to confirm their endothelial identity and functional properties.

Related

  1. Initial clonogenic potential of human endothelial progenitor cells is predictive of their further properties and establishes a functional hierarchy related to immaturity
    Ségolène Ferratge, Guillaume Ha, Gilles Carpentier +5
    Stem Cell ResearchPeer-reviewedCC BY-NC-NDFull text44 citedMolecular biology & gene regulationHAL
  2. Protocol for endothelial cell-derived extracellular vesicle isolation and characterization.
    Charlotte Hooper, Naveed Akbar
    STAR protocolsPeer-reviewedCC BYFull textMolecular biology & gene regulationEurope PMC
  3. Astaxanthin Inhibits ox-LDL-Induced Early Endothelial Progenitor Cells Oxidative Stress and Apoptosis Through Nrf2 Activation.
    Keqing Pan, Xiao Xu, Qijun Jiang +4
    Stem cells internationalPeer-reviewedCC BYFull textMolecular biology & gene regulationEurope PMCPMC
  4. LNP-mediated CRISPR gene editing of human vascular endothelial cells in 2D and 3D microfluidic cell culture systems
    Amalie Lykke Olsen, Camilla Blunk Vorsø Brandt, Rasmus Karred Larsen +11
    Molecular therapy. Nucleic acidsPeer-reviewedCC BY-NC-NDFull textMolecular biology & gene regulationEurope PMCEurope PMCPMC
  5. Fluorescence-activated nuclei sorting (FANS) on human post-mortem cortex tissue enabling the isolation of distinct neural cell populations for multiple omic profiling v2
    Stefania S S Policicchio, Barry A. Chioza, Jonathan P. Davies +3
    PreprintCC BYFull text9 citedMolecular biology & gene regulationOpenAlex

Cite

BibTeXRIS
BibTeX
@article{abbasimalati2026isolation,
  title = {{Isolation and in vitro culture of endothelial progenitor cell-like populations from leukoreduction filters}},
  author = {Zahra Abbasi-Malati and Seyed Ghader Azizi and Sahar Balagholi and Ali Akbar Pourfathollah},
  journal = {BMC Biotechnology},
  year = {2026},
  month = aug,
  doi = {10.1186/s12896-026-01209-y},
  url = {https://doi.org/10.1186/s12896-026-01209-y},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Isolation and in vitro culture of endothelial progenitor cell-like populations from leukoreduction filters
AU  - Zahra Abbasi-Malati
AU  - Seyed Ghader Azizi
AU  - Sahar Balagholi
AU  - Ali Akbar Pourfathollah
PY  - 2026
DA  - 2026/08/10
JO  - BMC Biotechnology
DO  - 10.1186/s12896-026-01209-y
UR  - https://doi.org/10.1186/s12896-026-01209-y
AB  - Background: Leukoreduction filters (LRFs) are routinely used in blood transfusion services and retain substantial numbers of nucleated cells during blood processing. These discarded filters have therefore been explored as a potential source of peripheral blood-derived cells for endothelial progenitor cell (EPC)-like related in vitro studies.  Methods: Leukoreduction filters were collected from the Iranian Blood Transfusion Organization (IBTO). Entrapped cells were recovered using a washing-based procedure without the use of magnetic-activated cell sorting (MACS) or endothelial differentiation media. A coating solution containing plasma-derived components, including cryoprecipitate and platelet lysate, was applied to improve early cell adhesion during in vitro culture.  Results: Cultured cells were characterized by flow cytometry using CD133 as a surface-associated progenitor marker. Approximately 70% of the cells at passage 3–4 was positive for CD133.  Conclusion: Leukoreduction filters may serve as an accessible and cost-effective source of peripheral blood-derived cell populations for EPC-like related in vitro studies. Further studies are required to confirm their endothelial identity and functional properties.
SN  - 1472-6750
ER  -