Peer-reviewedCC BYSTAR protocols
Protocol for endothelial cell-derived extracellular vesicle isolation and characterization.
- 1Division of Cardiovascular Medicine, Radcliffe Department of Medicine, University of Oxford, OX3 9DU Oxford, UK.
- 2Division of Cardiovascular Medicine, Radcliffe Department of Medicine, University of Oxford, OX3 9DU Oxford, UK. Electronic address: [email protected].
Abstract
Endothelial cells regulate intercellular and inter-organ communication through extracellular vesicles (EVs), which have diagnostic and therapeutic potential. We describe single-particle interferometric reflectance imaging directly from culture supernatants, followed by ultracentrifugation isolation of EVs. We determine EV size and concentration by nanoparticle tracking analysis and characterize EVs by western blotting and transmission electron microscopy. This protocol can be applied to other cell systems of EV biology. For details on the execution of this protocol, please refer to Timms et al.¹.
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BibTeX
@article{hooper2026protocol,
title = {{Protocol for endothelial cell-derived extracellular vesicle isolation and characterization.}},
author = {Charlotte Hooper and Naveed Akbar},
journal = {STAR protocols},
year = {2026},
month = oct,
doi = {10.1016/j.xpro.2026.104887},
url = {https://europepmc.org/article/MED/42858256},
note = {CC BY},
}
RIS
TY - JOUR
TI - Protocol for endothelial cell-derived extracellular vesicle isolation and characterization.
AU - Charlotte Hooper
AU - Naveed Akbar
PY - 2026
DA - 2026/10/09
JO - STAR protocols
DO - 10.1016/j.xpro.2026.104887
UR - https://europepmc.org/article/MED/42858256
AB - Endothelial cells regulate intercellular and inter-organ communication through extracellular vesicles (EVs), which have diagnostic and therapeutic potential. We describe single-particle interferometric reflectance imaging directly from culture supernatants, followed by ultracentrifugation isolation of EVs. We determine EV size and concentration by nanoparticle tracking analysis and characterize EVs by western blotting and transmission electron microscopy. This protocol can be applied to other cell systems of EV biology. For details on the execution of this protocol, please refer to Timms et al.¹.
SN - 2666-1667
ER -