LC-MS Identification and Preliminary Pharmacological Study of the Aqueous and Ethanol Extracts from Combretum glutinosum Perr ex DC. (Combretaceae)
Abstract
Combretum glutinosum is a plant whose leaves are consumed as a vegetable and used in traditional medicine for the treatment of microbial infections. The present study was designed to identify the compounds in C. glutinosum leaves extracts, and evaluate its antimicrobial activity, antioxidant ability and its toxicity in Artemia salina larvae in vitro. The aqueous and ethanol extracts obtained from the leaves of the plant as well as known compounds previously isolated and characterized from the leaves of C. glutinosum were tested on eleven (11) different microbial strains. The antioxidant activity of the extracts was evaluated by the Ferric Reducing Antioxidant Power method and the larval toxicity on Artemia salina larvae was also detected. Phytochemical screening and LC-MS analysis were performed on the extracts to characterize its chemical composition. When tested at a concentration of 20 mg.mL−1, the extracts of C. glutinosum leaves strongly inhibited the growth of the bacterial strains with an inhibition diameter ranging from 7.25 to 44 mm, superior to those of the positive controls (tetracycline at 30 µg.mL−1 and amikacin at 30 µg.mL−1), inhibition diameters from 15 to 33 mm. The evaluated larval toxicity demonstrated that it had no harmful effects on Artemia salina larvae. The extracts present a good antioxidant activity at a concentration of 0.17 and 1.33 mmol ascorbic acid (per gram of extract) for the aqueous and ethanol extracts, respectively. However, none of the compounds tested at 500 µg.mL−1 were able to show good activity on the 11 reference strains. Phytochemical analysis revealed the presence of alkaloids, polyphenols, steroids, triterpenoids, reducing compounds etc. in both extracts. The LC-MS analyses revealed 18 compounds in the ethanol extract, from which 3 were identified, 15 compounds in the aqueous extract from which 5 could be identified. The present work has shown that C. glutinosum extracts can be a good source of antimicrobial agents. They also possess the antioxidant property with absence of toxicity on A. salina larvae. A further bio-guided study could allow the identification and isolation of the active ingredients.
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BibTeX
@article{toklo2026lcms,
title = {{LC-MS Identification and Preliminary Pharmacological Study of the Aqueous and Ethanol Extracts from Combretum glutinosum Perr ex DC. (Combretaceae)}},
author = {Placide Mahougnan Toklo and Mathias Amour Ahomadegbe and Durand Dah-Nouvlessounon and Jean-Bosco Jouda and Billy Toussie Tchegnitegni and Steven Collins Njonte Wouamba and Djidénou Ahoton and Mahoudo Fidèle Assogba and Joseph Tchamgoue and Bruno Ndjakou Lenta and Simeon Fogue Kouam and Lamine Baba-Moussa and Eléonore Chikani Yayi Ladekan and Joachim Djimon Gbenou},
journal = {Clinical Complementary Medicine and Pharmacology},
year = {2026},
month = oct,
doi = {10.1016/j.ccmp.2023.100106},
url = {https://hal.science/hal-05790533v1},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - LC-MS Identification and Preliminary Pharmacological Study of the Aqueous and Ethanol Extracts from Combretum glutinosum Perr ex DC. (Combretaceae)
AU - Placide Mahougnan Toklo
AU - Mathias Amour Ahomadegbe
AU - Durand Dah-Nouvlessounon
AU - Jean-Bosco Jouda
AU - Billy Toussie Tchegnitegni
AU - Steven Collins Njonte Wouamba
AU - Djidénou Ahoton
AU - Mahoudo Fidèle Assogba
AU - Joseph Tchamgoue
AU - Bruno Ndjakou Lenta
AU - Simeon Fogue Kouam
AU - Lamine Baba-Moussa
AU - Eléonore Chikani Yayi Ladekan
AU - Joachim Djimon Gbenou
PY - 2026
DA - 2026/10/10
JO - Clinical Complementary Medicine and Pharmacology
DO - 10.1016/j.ccmp.2023.100106
UR - https://hal.science/hal-05790533v1
AB - Combretum glutinosum is a plant whose leaves are consumed as a vegetable and used in traditional medicine for the treatment of microbial infections. The present study was designed to identify the compounds in C. glutinosum leaves extracts, and evaluate its antimicrobial activity, antioxidant ability and its toxicity in Artemia salina larvae in vitro. The aqueous and ethanol extracts obtained from the leaves of the plant as well as known compounds previously isolated and characterized from the leaves of C. glutinosum were tested on eleven (11) different microbial strains. The antioxidant activity of the extracts was evaluated by the Ferric Reducing Antioxidant Power method and the larval toxicity on Artemia salina larvae was also detected. Phytochemical screening and LC-MS analysis were performed on the extracts to characterize its chemical composition. When tested at a concentration of 20 mg.mL−1, the extracts of C. glutinosum leaves strongly inhibited the growth of the bacterial strains with an inhibition diameter ranging from 7.25 to 44 mm, superior to those of the positive controls (tetracycline at 30 µg.mL−1 and amikacin at 30 µg.mL−1), inhibition diameters from 15 to 33 mm. The evaluated larval toxicity demonstrated that it had no harmful effects on Artemia salina larvae. The extracts present a good antioxidant activity at a concentration of 0.17 and 1.33 mmol ascorbic acid (per gram of extract) for the aqueous and ethanol extracts, respectively. However, none of the compounds tested at 500 µg.mL−1 were able to show good activity on the 11 reference strains. Phytochemical analysis revealed the presence of alkaloids, polyphenols, steroids, triterpenoids, reducing compounds etc. in both extracts. The LC-MS analyses revealed 18 compounds in the ethanol extract, from which 3 were identified, 15 compounds in the aqueous extract from which 5 could be identified. The present work has shown that C. glutinosum extracts can be a good source of antimicrobial agents. They also possess the antioxidant property with absence of toxicity on A. salina larvae. A further bio-guided study could allow the identification and isolation of the active ingredients.
SN - 2772-3712
SN - 2772-3712
ER -