Identification of active deubiquitinases in dendritic cells under unstimulated and activated conditions.
- 1The University of Melbourne
Abstract
Ubiquitination is a post-translational modification with well described roles in the regulation of immune cell function, such as dendritic cells (DCs). DCs are crucial initiators and regulators of T cell-mediated immune responses. In this study, we use activity-based probes and proteomics to systematically profile DUBs in a DC line. Using the lysate-based biotin(bio)-Ubiquitin(Ub)-propargylamide(Prg) probe and proteomics, the activity and abundance of deubiquitinases was measured in unstimulated and CpG-stimulated MutuDCs to simulate responses to bacteria. This analysis was complemented with profiling using the cell-permeable DUB activity-based probe IMP-2373. The combined analysis with bio-Ub-Prg and IMP-2373 provided insights into the dynamics of DUB activity and abundance in DCs following stimulation and identified several DUBs for further study.
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BibTeX
@misc{morgan2026identification,
title = {{Identification of active deubiquitinases in dendritic cells under unstimulated and activated conditions.}},
author = {H. B. Morgan and J. A. Villadangos and L. E. Edgington-Mitchell and J. D. Mintern},
howpublished = {bioRxiv},
year = {2026},
month = oct,
doi = {10.64898/2026.10.08.757166},
url = {https://www.biorxiv.org/content/10.64898/2026.10.08.757166v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Identification of active deubiquitinases in dendritic cells under unstimulated and activated conditions.
AU - H. B. Morgan
AU - J. A. Villadangos
AU - L. E. Edgington-Mitchell
AU - J. D. Mintern
PY - 2026
DA - 2026/10/08
PB - bioRxiv
DO - 10.64898/2026.10.08.757166
UR - https://www.biorxiv.org/content/10.64898/2026.10.08.757166v1
AB - Ubiquitination is a post-translational modification with well described roles in the regulation of immune cell function, such as dendritic cells (DCs). DCs are crucial initiators and regulators of T cell-mediated immune responses. In this study, we use activity-based probes and proteomics to systematically profile DUBs in a DC line. Using the lysate-based biotin(bio)-Ubiquitin(Ub)-propargylamide(Prg) probe and proteomics, the activity and abundance of deubiquitinases was measured in unstimulated and CpG-stimulated MutuDCs to simulate responses to bacteria. This analysis was complemented with profiling using the cell-permeable DUB activity-based probe IMP-2373. The combined analysis with bio-Ub-Prg and IMP-2373 provided insights into the dynamics of DUB activity and abundance in DCs following stimulation and identified several DUBs for further study.
ER -