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Peer-reviewedCC BY-NC-ND 4.0STAR Protocols

Protocol for monitoring cytoplasmic Ca2+ dynamics using Fura Red-AM on a Zeiss Axio Observer microscope

  • Sabrina Beaumier
  • Marc Bartoli
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Abstract

Protocol for monitoring cytoplasmic Ca 2+ dynamics using Fura Red-AM on a Zeiss Axio Observer microscope

Here, we present a protocol for live-cell calcium imaging in HEK, HeLa, fibroblast, and myoblast cells using Fura Red-AM and a Zeiss Axio Observer inverted microscope. We describe steps for dye preparation and loading, microscope setup, dual-excitation image acquisition, and ratiometric signal analysis. The setup is optimized for store-operated calcium entry (SOCE), based on the activation of STIM1 and ORAI1. This protocol enables real-time monitoring of intracellular calcium dynamics and supports both non-transfected and GFP-transfected cells.

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Cite

BibTeXRIS
BibTeX
@article{beaumier2026protocol,
  title = {{Protocol for monitoring cytoplasmic Ca2+ dynamics using Fura Red-AM on a Zeiss Axio Observer microscope}},
  author = {Sabrina Beaumier and Marc Bartoli},
  journal = {STAR Protocols},
  year = {2026},
  month = sep,
  doi = {10.1016/j.xpro.2026.104469},
  url = {https://hal.science/hal-05759938v1},
  note = {CC BY-NC-ND 4.0},
}
RIS
TY  - JOUR
TI  - Protocol for monitoring cytoplasmic Ca2+ dynamics using Fura Red-AM on a Zeiss Axio Observer microscope
AU  - Sabrina Beaumier
AU  - Marc Bartoli
PY  - 2026
DA  - 2026/09/23
JO  - STAR Protocols
DO  - 10.1016/j.xpro.2026.104469
UR  - https://hal.science/hal-05759938v1
AB  - Protocol for monitoring cytoplasmic Ca 2+ dynamics using Fura Red-AM on a Zeiss Axio Observer microscope  Here, we present a protocol for live-cell calcium imaging in HEK, HeLa, fibroblast, and myoblast cells using Fura Red-AM and a Zeiss Axio Observer inverted microscope. We describe steps for dye preparation and loading, microscope setup, dual-excitation image acquisition, and ratiometric signal analysis. The setup is optimized for store-operated calcium entry (SOCE), based on the activation of STIM1 and ORAI1. This protocol enables real-time monitoring of intracellular calcium dynamics and supports both non-transfected and GFP-transfected cells.
SN  - 2666-1667
ER  -