BioID Performed on Golgi Enriched Fractions Identify C10orf76 as a GBF1 Binding Protein Essential for Golgi Maintenance and Secretion
Abstract
The Golgi-specific Brefeldin-A resistance factor 1 (GBF1) is the only large GEF that regulates Arf activation at the cis- Golgi and is actively recruited to membranes on an increase in Arf-GDP. Recent studies have revealed that GBF1 recruitment requires one or more heat-labile and protease-sensitive protein factor(s) (Quilty et al. , 2018, J. Cell Science, 132). Proximity-dependent biotinylation (BioID) and mass spectrometry from enriched Golgi fractions identified GBF1 proximal proteins that may regulate its recruitment. Knockdown studies revealed C10orf76 to be involved in Golgi maintenance. We find that C10orf76 interacts with GBF1 and rapidly cycles on and off GBF1-positive Golgi structures. More importantly, its depletion causes Golgi fragmentation, alters GBF1 recruitment, and impairs secretion. Homologs were identified in most species, suggesting its presence in the last eukaryotic common ancestor.
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BibTeX
@article{chan2026bioid,
title = {{BioID Performed on Golgi Enriched Fractions Identify C10orf76 as a GBF1 Binding Protein Essential for Golgi Maintenance and Secretion}},
author = {Calvin Chan and Roberta Le and Kaylan Burns and Khadra Ahmed and Etienne Coyaud and Estelle M.N. Laurent and Brian Raught and Paul Melançon},
journal = {Molecular and Cellular Proteomics},
year = {2026},
month = oct,
doi = {10.1074/mcp.ra119.001645},
url = {https://hal.science/hal-05790888v1},
note = {CC BY},
}
RIS
TY - JOUR
TI - BioID Performed on Golgi Enriched Fractions Identify C10orf76 as a GBF1 Binding Protein Essential for Golgi Maintenance and Secretion
AU - Calvin Chan
AU - Roberta Le
AU - Kaylan Burns
AU - Khadra Ahmed
AU - Etienne Coyaud
AU - Estelle M.N. Laurent
AU - Brian Raught
AU - Paul Melançon
PY - 2026
DA - 2026/10/10
JO - Molecular and Cellular Proteomics
DO - 10.1074/mcp.ra119.001645
UR - https://hal.science/hal-05790888v1
AB - The Golgi-specific Brefeldin-A resistance factor 1 (GBF1) is the only large GEF that regulates Arf activation at the cis- Golgi and is actively recruited to membranes on an increase in Arf-GDP. Recent studies have revealed that GBF1 recruitment requires one or more heat-labile and protease-sensitive protein factor(s) (Quilty et al. , 2018, J. Cell Science, 132). Proximity-dependent biotinylation (BioID) and mass spectrometry from enriched Golgi fractions identified GBF1 proximal proteins that may regulate its recruitment. Knockdown studies revealed C10orf76 to be involved in Golgi maintenance. We find that C10orf76 interacts with GBF1 and rapidly cycles on and off GBF1-positive Golgi structures. More importantly, its depletion causes Golgi fragmentation, alters GBF1 recruitment, and impairs secretion. Homologs were identified in most species, suggesting its presence in the last eukaryotic common ancestor.
SN - 1535-9476
SN - 1535-9484
ER -