Evaluation of a Novel Antioxidant-supplemented Sperm Wash Medium on Human Sperm Function: A Pilot Study.
7 affiliations
- 1Department of Anatomy, Faculty of Medicine, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, Tamil Nadu, India.
- 2Crea Conceptions Pvt Ltd., Chennai, Tamil Nadu, India.
- 3Department of Anatomy, Annaii Medical College, Rajalakshmi Health City, Kanchipuram, Tamil Nadu, India.
- 4Sathyabama Institute of Science and Technology, Chennai, Tamil Nadu, India.
- 5Faculty of Pharmacy, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, Tamil Nadu, India.
- 6Department of Biomedical Sciences, Faculty of Biomedical Sciences and Technology, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, Tamil Nadu, India.
- 7Department of Reproductive Medicine and Surgery, Sri Ramachandra Institute of Higher Education and Research (DU), Chennai, Tamil Nadu, India.
Abstract
Background
Density gradient sperm preparation may induce oxidative stress, impairing sperm function and deoxyribonucleic acid (DNA) integrity.Aim
To formulate and test an antioxidant-supplemented sperm wash with proteins.Settings and design
An in vitro pilot proof of concept with a paired sample design.Materials and methods
Ten normal and 10 abnormal semen samples were processed by double density gradient centrifugation of the sperm and then split into two portions that were placed in basal Ham's F10 and in an antioxidant-enriched medium (L-carnitine, N-acetyl cysteine and D-aspartic acid). Reactive oxygen species (ROS), motility, total motile sperm count (TMSC), morphology, teratozoospermic index (TZI) and DNA fragmentation were evaluated. High-performance liquid chromatography, nuclear magnetic resonance, molecular docking and molecular dynamics analyses were done.Statistical analyses used
ANOVA with Šidák/Tukey post hoc variations.Results
ROS (P<0.0001) and DNA fragmentation index (P< 0.001) levels were found to be significantly reduced with antioxidant supplementation. The motility with progressive movement and TMSC was still maintained (P< 0.01), especially in teratozoospermic specimens. Sperm recovery was similar for all media, and there were slight changes in morphology and in TZI. In silico results showed that binding to sperm function-associated protein targets was stable.Conclusion
The findings of this study indicate that antioxidant supplementation can decrease oxidative stress and improve sperm function during preparation. Studies with more subjects that include fertilisation, embryo development, pregnancy and live-birth outcomes are needed to validate.
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BibTeX
@article{enmozhi2026evaluation,
title = {{Evaluation of a Novel Antioxidant-supplemented Sperm Wash Medium on Human Sperm Function: A Pilot Study.}},
author = {Sukanth Kumar Enmozhi and Sasikala Natarajamani and Gunapriya Raghunath and Jerrine Joseph and Infant Xavier and Theepan Raaj and S Pradeep and Niloy Raj Kalita and Sumathy Arockiasamy and Radha Vembu and Ananda Rani},
journal = {Journal of human reproductive sciences},
year = {2026},
month = jul,
doi = {10.4103/jhrs.jhrs_32_26},
url = {https://europepmc.org/article/MED/42923895},
note = {CC BY-NC-SA},
}
RIS
TY - JOUR
TI - Evaluation of a Novel Antioxidant-supplemented Sperm Wash Medium on Human Sperm Function: A Pilot Study.
AU - Sukanth Kumar Enmozhi
AU - Sasikala Natarajamani
AU - Gunapriya Raghunath
AU - Jerrine Joseph
AU - Infant Xavier
AU - Theepan Raaj
AU - S Pradeep
AU - Niloy Raj Kalita
AU - Sumathy Arockiasamy
AU - Radha Vembu
AU - Ananda Rani
PY - 2026
DA - 2026/07/01
JO - Journal of human reproductive sciences
DO - 10.4103/jhrs.jhrs_32_26
UR - https://europepmc.org/article/MED/42923895
AB - Background Density gradient sperm preparation may induce oxidative stress, impairing sperm function and deoxyribonucleic acid (DNA) integrity.Aim To formulate and test an antioxidant-supplemented sperm wash with proteins.Settings and design An in vitro pilot proof of concept with a paired sample design.Materials and methods Ten normal and 10 abnormal semen samples were processed by double density gradient centrifugation of the sperm and then split into two portions that were placed in basal Ham's F10 and in an antioxidant-enriched medium (L-carnitine, N-acetyl cysteine and D-aspartic acid). Reactive oxygen species (ROS), motility, total motile sperm count (TMSC), morphology, teratozoospermic index (TZI) and DNA fragmentation were evaluated. High-performance liquid chromatography, nuclear magnetic resonance, molecular docking and molecular dynamics analyses were done.Statistical analyses used ANOVA with Šidák/Tukey post hoc variations.Results ROS (P<0.0001) and DNA fragmentation index (P< 0.001) levels were found to be significantly reduced with antioxidant supplementation. The motility with progressive movement and TMSC was still maintained (P< 0.01), especially in teratozoospermic specimens. Sperm recovery was similar for all media, and there were slight changes in morphology and in TZI. In silico results showed that binding to sperm function-associated protein targets was stable.Conclusion The findings of this study indicate that antioxidant supplementation can decrease oxidative stress and improve sperm function during preparation. Studies with more subjects that include fertilisation, embryo development, pregnancy and live-birth outcomes are needed to validate.
SN - 0974-1208
SN - 1998-4766
ER -