B vitamins and metabolic dysfunction-associated steatotic liver disease (MASLD): a systematic review of observational studies.
5 affiliations
- 1Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran.
- 2Endocrine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
- 3Department of Chemical and Biochemical Engineering, Western University, London, ON, N6A 5B9, Canada.
- 4Department of Biochemistry and Diet Therapy, Faculty of Nutrition & Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran.
- 5Nutrition Research Center, Department of Biochemistry and Diet Therapy, Faculty of Nutrition and Food Sciences, Tabriz University of Medical Sciences, Tabriz, Iran. [email protected].
Abstract
Background
B vitamins are crucial cofactors in metabolic regulation; however, their role in the pathogenesis of metabolic dysfunction-associated steatotic liver disease (MASLD) remains unclear. Evidence for individual B vitamins is often contradictory. This systematic review evaluated the findings of observational studies examining the association between B vitamin intake and MASLD risk, severity, and outcomes.Methods
A systematic search was conducted in PubMed, Scopus, Web of Science, and Google Scholar for studies published up to January 2026, adhering to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. All included studies were observational in design, encompassing cross-sectional, case-control, cohort, and Mendelian randomization (MR) studies that assessed dietary, supplemental, or biological B-vitamin status in relation to MASLD diagnoses. Study quality was evaluated using the Newcastle-Ottawa scale (NOS). No quantitative meta-analysis was performed and the evidence was narratively synthesized.Results
Fifty-nine studies were included (38 cross-sectional, 13 case-control, 6 cohort, and 2 MR studies). Folate has been extensively studied, with most evidence suggesting a protective role for serum folate and 5-mTHF against MASLD and fibrosis. However, this was contradicted by positive associations for RBC folate and unmetabolized folic acid (UMFA). Cohort studies have linked higher folate status to reduced all-cause mortality. Vitamin B12 findings were highly controversial, with studies reporting protective, detrimental, and null associations; MR studies suggested a positive causal link with MASLD risk. Choline intake was inversely associated with steatosis but positively correlated with fibrosis in specific subgroups, including men and postmenopausal women. Evidence for niacin and pyridoxine was inconsistent, though often suggesting an inverse relationship with MASLD.Conclusion
Observational evidence suggests significant, albeit contradictory, associations between specific B-vitamins (particularly folate and B12), choline, and MASLD progression. However, given the massive methodological heterogeneity and heavy reliance on cross-sectional data, the certainty of these findings is very low. The inherent uncertainty of these associations rather than concluding a probable clinical benefit should also be emphasized. Rigorous prospective cohorts and randomized controlled trials are required to establish causality and clarify the true clinical utility of B-vitamin status in MASLD.Trial registration
Not applicable.
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BibTeX
@article{safargar2026vitamins,
title = {{B vitamins and metabolic dysfunction-associated steatotic liver disease (MASLD): a systematic review of observational studies.}},
author = {Mohammad Safargar and Sara Arefhosseini and Helda Tutunchi and Seyed Rafie Arefhosseini and Mehrangiz Ebrahimi-Mameghani},
journal = {BMC endocrine disorders},
year = {2026},
month = jul,
doi = {10.1186/s12902-026-02420-w},
url = {https://europepmc.org/article/MED/42907789},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - B vitamins and metabolic dysfunction-associated steatotic liver disease (MASLD): a systematic review of observational studies.
AU - Mohammad Safargar
AU - Sara Arefhosseini
AU - Helda Tutunchi
AU - Seyed Rafie Arefhosseini
AU - Mehrangiz Ebrahimi-Mameghani
PY - 2026
DA - 2026/07/01
JO - BMC endocrine disorders
DO - 10.1186/s12902-026-02420-w
UR - https://europepmc.org/article/MED/42907789
AB - Background B vitamins are crucial cofactors in metabolic regulation; however, their role in the pathogenesis of metabolic dysfunction-associated steatotic liver disease (MASLD) remains unclear. Evidence for individual B vitamins is often contradictory. This systematic review evaluated the findings of observational studies examining the association between B vitamin intake and MASLD risk, severity, and outcomes.Methods A systematic search was conducted in PubMed, Scopus, Web of Science, and Google Scholar for studies published up to January 2026, adhering to Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines. All included studies were observational in design, encompassing cross-sectional, case-control, cohort, and Mendelian randomization (MR) studies that assessed dietary, supplemental, or biological B-vitamin status in relation to MASLD diagnoses. Study quality was evaluated using the Newcastle-Ottawa scale (NOS). No quantitative meta-analysis was performed and the evidence was narratively synthesized.Results Fifty-nine studies were included (38 cross-sectional, 13 case-control, 6 cohort, and 2 MR studies). Folate has been extensively studied, with most evidence suggesting a protective role for serum folate and 5-mTHF against MASLD and fibrosis. However, this was contradicted by positive associations for RBC folate and unmetabolized folic acid (UMFA). Cohort studies have linked higher folate status to reduced all-cause mortality. Vitamin B12 findings were highly controversial, with studies reporting protective, detrimental, and null associations; MR studies suggested a positive causal link with MASLD risk. Choline intake was inversely associated with steatosis but positively correlated with fibrosis in specific subgroups, including men and postmenopausal women. Evidence for niacin and pyridoxine was inconsistent, though often suggesting an inverse relationship with MASLD.Conclusion Observational evidence suggests significant, albeit contradictory, associations between specific B-vitamins (particularly folate and B12), choline, and MASLD progression. However, given the massive methodological heterogeneity and heavy reliance on cross-sectional data, the certainty of these findings is very low. The inherent uncertainty of these associations rather than concluding a probable clinical benefit should also be emphasized. Rigorous prospective cohorts and randomized controlled trials are required to establish causality and clarify the true clinical utility of B-vitamin status in MASLD.Trial registration Not applicable.
SN - 1472-6823
ER -