Exploratory Transcriptomic Profiling of Vein Tissue from Diabetic Patients Undergoing Arteriovenous Fistula Creation for Hemodialysis
Abstract
AIM: Arteriovenous fistula (AVF) is the preferred vascular access for hemodialysis; however, its patency is limited, particularly in patients with diabetes mellitus (DM). This study aimed to obtain exploratory data on whether changes in the venous transcriptome are present at the time of AVF creation in patients with DM and whether these changes are related to the graft prognosis. METHODS: We conducted prospective transcriptome analyses using venous samples obtained during AVF creation from 22 patients (12 with DM and 10 without DM). RNA sequencing was performed, and clinical outcomes, including primary patency, were tracked for one year. RESULTS: AVF patency was significantly lower in the DM group (median patency: 134 vs. 365 days, p＜0.05). Transcriptome profiling identified 285 differentially expressed genes, including those related to extracellular matrix organization, mitochondrial electron transport, TGF-β signaling, and the unfolded protein response. A network analysis highlighted hub genes, including MRPL27, MRPL23, and MRPL1, as potential biomarkers. A Cox regression analysis identified genes associated with the AVF prognosis, including PER3, SERINC2, COMMD10, TTC4, FKBP2, FN3KRP, KIF24, SNX32, MOB3C, SUV39H1, and TIGD6. Notably, a high COMMD10 expression, a core component of the commander complex, was associated with a shortened patency in patients with DM. CONCLUSIONS: Our findings demonstrate that DM is associated with distinct transcriptomic alterations in the access veins at the time of AVF creation. This integrative approach provides novel insights into the molecular basis of DM-related AVF failure and the potential to identify novel therapeutic targets.
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BibTeX
@article{shirouzu2026exploratory,
title = {{Exploratory Transcriptomic Profiling of Vein Tissue from Diabetic Patients Undergoing Arteriovenous Fistula Creation for Hemodialysis}},
author = {Tomohiro Shirouzu and Jun-ichiro Koga and Eisuke Katafuchi and Yoichiro Nagai and Ryota Suga and Kenya Sanada and Hiromichi Ueno and Kazutoshi Nakazono and Emi Hasegawa and Tetsu Miyamoto and Toshiyuki Nakayama and Masaharu Kataoka},
journal = {Journal of Atherosclerosis and Thrombosis},
year = {2026},
month = aug,
doi = {10.5551/jat.66048},
url = {https://www.jstage.jst.go.jp/article/jat/advpub/0/advpub_66048/_article},
note = {CC BY-NC-SA},
}
RIS
TY - JOUR
TI - Exploratory Transcriptomic Profiling of Vein Tissue from Diabetic Patients Undergoing Arteriovenous Fistula Creation for Hemodialysis
AU - Tomohiro Shirouzu
AU - Jun-ichiro Koga
AU - Eisuke Katafuchi
AU - Yoichiro Nagai
AU - Ryota Suga
AU - Kenya Sanada
AU - Hiromichi Ueno
AU - Kazutoshi Nakazono
AU - Emi Hasegawa
AU - Tetsu Miyamoto
AU - Toshiyuki Nakayama
AU - Masaharu Kataoka
PY - 2026
DA - 2026/08/06
JO - Journal of Atherosclerosis and Thrombosis
DO - 10.5551/jat.66048
UR - https://www.jstage.jst.go.jp/article/jat/advpub/0/advpub_66048/_article
AB - AIM: Arteriovenous fistula (AVF) is the preferred vascular access for hemodialysis; however, its patency is limited, particularly in patients with diabetes mellitus (DM). This study aimed to obtain exploratory data on whether changes in the venous transcriptome are present at the time of AVF creation in patients with DM and whether these changes are related to the graft prognosis. METHODS: We conducted prospective transcriptome analyses using venous samples obtained during AVF creation from 22 patients (12 with DM and 10 without DM). RNA sequencing was performed, and clinical outcomes, including primary patency, were tracked for one year. RESULTS: AVF patency was significantly lower in the DM group (median patency: 134 vs. 365 days, p＜0.05). Transcriptome profiling identified 285 differentially expressed genes, including those related to extracellular matrix organization, mitochondrial electron transport, TGF-β signaling, and the unfolded protein response. A network analysis highlighted hub genes, including MRPL27, MRPL23, and MRPL1, as potential biomarkers. A Cox regression analysis identified genes associated with the AVF prognosis, including PER3, SERINC2, COMMD10, TTC4, FKBP2, FN3KRP, KIF24, SNX32, MOB3C, SUV39H1, and TIGD6. Notably, a high COMMD10 expression, a core component of the commander complex, was associated with a shortened patency in patients with DM. CONCLUSIONS: Our findings demonstrate that DM is associated with distinct transcriptomic alterations in the access veins at the time of AVF creation. This integrative approach provides novel insights into the molecular basis of DM-related AVF failure and the potential to identify novel therapeutic targets.
SN - 1880-3873
SN - 1340-3478
ER -