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Peer-reviewedCC BYActa cirurgica brasileira

Protocol for bilateral oophorectomy in mice: a reliable and cost-effective model for promoting an obesogenic state.

  • Juliana Andrade Pereira1
  • Alice Crespo Ferreira1
  • Thaísa Soares Crespo1,2
  • Deborah de Farias Lelis1,2
  • Vera Lúcia Lacerda Medeiros1
  • João Marcus Oliveira Andrade1,2,3
  1. 1Universidade Estadual de Montes Claros - Programa de Pós-Graduação em Ciências da Saúde - Montes Claros (MG) - Brazil.
  2. 2Universidade Estadual de Montes Claros - Faculdade de Medicina - Departamento de Fisiopatologia - Montes Claros (MG) - Brazil.
  3. 3Universidade Federal de Minas Gerais - Programa de Pós-Graduação em Alimentos e Saúde - Montes Claros (MG) - Brazil.
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Abstract

Purpose

To describe a low-cost bilateral oophorectomy technique and evaluate its effects on adiposity and metabolic alterations in female mice.Methods

An experimental design was used with 16 female mice divided into two groups: control (laparotomy; n = 8) and oophorectomy (OOF; n = 8). After anesthetic induction and antisepsis, the procedure involved a medial abdominal incision, identification, and removal of the ovaries, and closure of the abdominal cavity. Animals were monitored for 12 weeks, assessing body weight, food intake, mortality, surgical complications, and biochemical parameters.Results

The animals in the OOF group exhibited significant increases in body weight and visceral adiposity, despite consuming similar amounts of food and energy compared to the control group. Moreover, the OOF group exhibited alterations in biochemical metabolic parameters, characterized by elevated levels of total cholesterol, low-density lipoprotein cholesterol (LDL-c), and glucose. No mortality was observed among the operated animals. In addition, no early or late postoperative complications were detected in either group. The average duration of the surgical procedure was approximately 7.45 minutes per mouse.Conclusion

The bilateral oophorectomy protocol is simple, low-cost, and requires minimal infrastructure. It represents a reliable experimental model for promoting an obesogenic state and metabolic alterations in female mice.

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Cite

BibTeXRIS
BibTeX
@article{pereira2026protocol,
  title = {{Protocol for bilateral oophorectomy in mice: a reliable and cost-effective model for promoting an obesogenic state.}},
  author = {Juliana Andrade Pereira and Alice Crespo Ferreira and Thaísa Soares Crespo and Deborah de Farias Lelis and Vera Lúcia Lacerda Medeiros and João Marcus Oliveira Andrade},
  journal = {Acta cirurgica brasileira},
  year = {2026},
  month = oct,
  doi = {10.1590/acb416126},
  url = {https://europepmc.org/article/MED/42912007},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Protocol for bilateral oophorectomy in mice: a reliable and cost-effective model for promoting an obesogenic state.
AU  - Juliana Andrade Pereira
AU  - Alice Crespo Ferreira
AU  - Thaísa Soares Crespo
AU  - Deborah de Farias Lelis
AU  - Vera Lúcia Lacerda Medeiros
AU  - João Marcus Oliveira Andrade
PY  - 2026
DA  - 2026/10/19
JO  - Acta cirurgica brasileira
DO  - 10.1590/acb416126
UR  - https://europepmc.org/article/MED/42912007
AB  - Purpose  To describe a low-cost bilateral oophorectomy technique and evaluate its effects on adiposity and metabolic alterations in female mice.Methods  An experimental design was used with 16 female mice divided into two groups: control (laparotomy; n = 8) and oophorectomy (OOF; n = 8). After anesthetic induction and antisepsis, the procedure involved a medial abdominal incision, identification, and removal of the ovaries, and closure of the abdominal cavity. Animals were monitored for 12 weeks, assessing body weight, food intake, mortality, surgical complications, and biochemical parameters.Results  The animals in the OOF group exhibited significant increases in body weight and visceral adiposity, despite consuming similar amounts of food and energy compared to the control group. Moreover, the OOF group exhibited alterations in biochemical metabolic parameters, characterized by elevated levels of total cholesterol, low-density lipoprotein cholesterol (LDL-c), and glucose. No mortality was observed among the operated animals. In addition, no early or late postoperative complications were detected in either group. The average duration of the surgical procedure was approximately 7.45 minutes per mouse.Conclusion  The bilateral oophorectomy protocol is simple, low-cost, and requires minimal infrastructure. It represents a reliable experimental model for promoting an obesogenic state and metabolic alterations in female mice.
SN  - 0102-8650
SN  - 1678-2674
ER  -