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Peer-reviewedCC BY-NCChembiochem : a European journal of chemical biology

Expression of Human CYP2D6 in Artificial Cells Based on Polymer-Lipid Hybrid Vesicles.

  • Sarah Dahl1,2
  • Paula De Dios Andres1
  • Grégory Beaune3
  • Jaakko V I Timonen3
  • James Behrendorff2
  • Kirill Alexandrov2
  • Wayne Johnston2
  • Brigitte Städler1
  1. 1Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Aarhus, Denmark.
  2. 2School of Biology and Environmental Science, Queensland University of Technology, Brisbane, Australia.
  3. 3Department of Applied Physics, Aalto University School of Science, Espoo, Finland.
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Abstract

Artificial cells with the ability to express functional, biomedically relevant enzymes have long been a goal in the field of bottom-up synthetic biology. Here, we demonstrate the expression of human cytochrome P450 2D6 (CYP2D6) within the lumen of giant hybrid vesicle-based artificial cells using a cell-free protein expression system based on the protozoan Leishmania tarentolae. The vesicle membranes comprise 1,2-dioleoyl-sn-glycero-3-phosphocholine and amphiphilic block copolymers containing poly(cholesteryl methacrylate-co-butyl methacrylate) with either poly(galactose methacrylate) (pGalacMA) or poly(carboxyethyl acrylate) as the hydrophilic block. The amount and composition of the block copolymer incorporated into the giant hybrid vesicle membrane altered the artificial cells' ability to support functional protein expression, likely through changes in membrane properties. In particular, giant hybrid vesicles containing pGalacMA supported production of enzymatically active CYP2D6. Overall, the reported approach advances the development of artificial cells capable of producing functional, biomedically relevant enzymes. Further, the effort highlights complex interplay between polymer properties and performance of biological components in the functional complexity achievable in polymer-lipid hybrid vesicles.

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Cite

BibTeXRIS
BibTeX
@article{dahl2026expression,
  title = {{Expression of Human CYP2D6 in Artificial Cells Based on Polymer-Lipid Hybrid Vesicles.}},
  author = {Sarah Dahl and Paula De Dios Andres and Grégory Beaune and Jaakko V I Timonen and James Behrendorff and Kirill Alexandrov and Wayne Johnston and Brigitte Städler},
  journal = {Chembiochem : a European journal of chemical biology},
  year = {2026},
  month = oct,
  doi = {10.1002/cbic.70572},
  url = {https://europepmc.org/article/MED/42960696},
  note = {CC BY-NC},
}
RIS
TY  - JOUR
TI  - Expression of Human CYP2D6 in Artificial Cells Based on Polymer-Lipid Hybrid Vesicles.
AU  - Sarah Dahl
AU  - Paula De Dios Andres
AU  - Grégory Beaune
AU  - Jaakko V I Timonen
AU  - James Behrendorff
AU  - Kirill Alexandrov
AU  - Wayne Johnston
AU  - Brigitte Städler
PY  - 2026
DA  - 2026/10/30
JO  - Chembiochem : a European journal of chemical biology
DO  - 10.1002/cbic.70572
UR  - https://europepmc.org/article/MED/42960696
AB  - Artificial cells with the ability to express functional, biomedically relevant enzymes have long been a goal in the field of bottom-up synthetic biology. Here, we demonstrate the expression of human cytochrome P450 2D6 (CYP2D6) within the lumen of giant hybrid vesicle-based artificial cells using a cell-free protein expression system based on the protozoan Leishmania tarentolae. The vesicle membranes comprise 1,2-dioleoyl-sn-glycero-3-phosphocholine and amphiphilic block copolymers containing poly(cholesteryl methacrylate-co-butyl methacrylate) with either poly(galactose methacrylate) (pGalacMA) or poly(carboxyethyl acrylate) as the hydrophilic block. The amount and composition of the block copolymer incorporated into the giant hybrid vesicle membrane altered the artificial cells' ability to support functional protein expression, likely through changes in membrane properties. In particular, giant hybrid vesicles containing pGalacMA supported production of enzymatically active CYP2D6. Overall, the reported approach advances the development of artificial cells capable of producing functional, biomedically relevant enzymes. Further, the effort highlights complex interplay between polymer properties and performance of biological components in the functional complexity achievable in polymer-lipid hybrid vesicles.
SN  - 1439-4227
SN  - 1439-7633
ER  -