The Screening and Identification of Potential Probiotics in Misgurnus anguillicaudatus Intestine
- 1School of Life Science, Liaoning Normal University, Dalian 116029, China
- 2Key Laboratory of Loach Genetics and Breeding of Jiangsu Province, Soochow University, Suzhou 215123, China
- 3School of Basic Medical Sciences, Suzhou Medical College of Soochow University, Suzhou 215123, China
Abstract
We characterized the intestinal microbiota of Misgurnus anguillicaudatus using 16S rRNA gene sequencing (V4 region). Strains secreting extracellular digestive enzymes were functionally screened, identified by 16S rRNA sequencing, and evaluated for biosafety. Microbial diversity analysis revealed that the intestinal microbiota of M. anguillicaudatus was dominated by the genera Vibrio, Pseudoalteromonas, and Aeromonas as core predominant taxa. Functional screening yielded four strains. Three non-hemolytic strains secreting amylase, lipase, and protease were identified as Aeromonas hydrophila, Shewanella xiamenensis, and Cedecea neteri, respectively. A. veronii was excluded due to its β-hemolytic activity. Biosafety assessment indicated that all three strains exhibited satisfactory biosafety. The three strains, A. hydrophila, S. xiamenensis, and C. neteri, obtained in this study possess potential probiotic functions in aiding host digestion and promoting nutrient absorption, and provide candidate strains for the development of microecological preparations for aquatic animals.
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BibTeX
@article{sui2026screening,
title = {{The Screening and Identification of Potential Probiotics in Misgurnus anguillicaudatus Intestine}},
author = {Zhaoxin Sui and Mingshuai Shu and Ruining Zhang and Jianing Yu and Zhuo Dai and Yi Sun and Jun Li},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27209097},
url = {https://www.mdpi.com/1422-0067/27/20/9097},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - The Screening and Identification of Potential Probiotics in Misgurnus anguillicaudatus Intestine
AU - Zhaoxin Sui
AU - Mingshuai Shu
AU - Ruining Zhang
AU - Jianing Yu
AU - Zhuo Dai
AU - Yi Sun
AU - Jun Li
PY - 2026
DA - 2026/10/14
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27209097
UR - https://www.mdpi.com/1422-0067/27/20/9097
AB - We characterized the intestinal microbiota of Misgurnus anguillicaudatus using 16S rRNA gene sequencing (V4 region). Strains secreting extracellular digestive enzymes were functionally screened, identified by 16S rRNA sequencing, and evaluated for biosafety. Microbial diversity analysis revealed that the intestinal microbiota of M. anguillicaudatus was dominated by the genera Vibrio, Pseudoalteromonas, and Aeromonas as core predominant taxa. Functional screening yielded four strains. Three non-hemolytic strains secreting amylase, lipase, and protease were identified as Aeromonas hydrophila, Shewanella xiamenensis, and Cedecea neteri, respectively. A. veronii was excluded due to its β-hemolytic activity. Biosafety assessment indicated that all three strains exhibited satisfactory biosafety. The three strains, A. hydrophila, S. xiamenensis, and C. neteri, obtained in this study possess potential probiotic functions in aiding host digestion and promoting nutrient absorption, and provide candidate strains for the development of microecological preparations for aquatic animals.
SN - 1422-0067
ER -