A 3D-Printed BCP/PLGA-PEG-PLGA Scaffold for LNC000133-Loaded BMSC-Derived Exosome Delivery Promotes Repair of Steroid-Induced Osteonecrosis of the Femoral Head
- 1Department of Orthopaedics, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
- 2Research Center for Clinical Medicine, First Affiliated Hospital of Kunming Medical University, Kunming 650032, China
Abstract
Dysfunction of bone marrow mesenchymal stem cells (BMSCs) and impaired exosome-mediated intercellular communication are recognized as critical contributors to the pathogenesis of steroid-induced osteonecrosis of the femoral head (SONFH), although the underlying mechanisms remain incompletely understood. Moreover, the development of novel biomaterials and therapeutic strategies capable of improving SONFH treatment remains an urgent clinical priority. Previous studies have demonstrated that LNC000133-modified exosomes derived from human BMSCs (hBMSCs) promote osteoblastic differentiation and enhance exosome uptake by osteoblasts, thereby exerting therapeutic effects in SONFH. Building on these findings, we developed a 3D-printed biphasic calcium phosphate (BCP)/poly (lactic-co-glycolic acid) (PLGA)- polyethylene glycol (PEG)-PLGA scaffold loaded with LNC000133-modified hBMSC-derived exosomes (LNC000133-Exos) to facilitate the clinical translation of this gene-based therapeutic approach for SONFH. The biological effects of this composite scaffold on hBMSCs were subsequently evaluated in vitro. Furthermore, the BCP/PLGA-PEG-PLGA scaffold loaded with LNC000133-Exos was applied for the treatment of SONFH in a rabbit model. Our results demonstrated that transplantation of the LNC000133-Exos-loaded BCP/PLGA-PEG-PLGA scaffold markedly enhanced bone repair and structural reconstruction within the necrotic region of the femoral head in vivo. Collectively, these findings indicate that the BCP/PLGA-PEG-PLGA scaffold and LNC000133 synergistically promote hBMSC proliferation and osteogenic differentiation while suppressing adipogenic differentiation, providing a strong theoretical foundation and a promising therapeutic strategy for targeted SONFH treatment.
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BibTeX
@article{xia20263dprinted,
title = {{A 3D-Printed BCP/PLGA-PEG-PLGA Scaffold for LNC000133-Loaded BMSC-Derived Exosome Delivery Promotes Repair of Steroid-Induced Osteonecrosis of the Femoral Head}},
author = {Tixiong Xia and Chengbin Yang and Xi Li and Tong Chen and Yingxing Xu},
journal = {International Journal of Molecular Sciences},
year = {2026},
month = oct,
doi = {10.3390/ijms27209277},
url = {https://www.mdpi.com/1422-0067/27/20/9277},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - A 3D-Printed BCP/PLGA-PEG-PLGA Scaffold for LNC000133-Loaded BMSC-Derived Exosome Delivery Promotes Repair of Steroid-Induced Osteonecrosis of the Femoral Head
AU - Tixiong Xia
AU - Chengbin Yang
AU - Xi Li
AU - Tong Chen
AU - Yingxing Xu
PY - 2026
DA - 2026/10/01
JO - International Journal of Molecular Sciences
DO - 10.3390/ijms27209277
UR - https://www.mdpi.com/1422-0067/27/20/9277
AB - Dysfunction of bone marrow mesenchymal stem cells (BMSCs) and impaired exosome-mediated intercellular communication are recognized as critical contributors to the pathogenesis of steroid-induced osteonecrosis of the femoral head (SONFH), although the underlying mechanisms remain incompletely understood. Moreover, the development of novel biomaterials and therapeutic strategies capable of improving SONFH treatment remains an urgent clinical priority. Previous studies have demonstrated that LNC000133-modified exosomes derived from human BMSCs (hBMSCs) promote osteoblastic differentiation and enhance exosome uptake by osteoblasts, thereby exerting therapeutic effects in SONFH. Building on these findings, we developed a 3D-printed biphasic calcium phosphate (BCP)/poly (lactic-co-glycolic acid) (PLGA)- polyethylene glycol (PEG)-PLGA scaffold loaded with LNC000133-modified hBMSC-derived exosomes (LNC000133-Exos) to facilitate the clinical translation of this gene-based therapeutic approach for SONFH. The biological effects of this composite scaffold on hBMSCs were subsequently evaluated in vitro. Furthermore, the BCP/PLGA-PEG-PLGA scaffold loaded with LNC000133-Exos was applied for the treatment of SONFH in a rabbit model. Our results demonstrated that transplantation of the LNC000133-Exos-loaded BCP/PLGA-PEG-PLGA scaffold markedly enhanced bone repair and structural reconstruction within the necrotic region of the femoral head in vivo. Collectively, these findings indicate that the BCP/PLGA-PEG-PLGA scaffold and LNC000133 synergistically promote hBMSC proliferation and osteogenic differentiation while suppressing adipogenic differentiation, providing a strong theoretical foundation and a promising therapeutic strategy for targeted SONFH treatment.
SN - 1422-0067
ER -