Serum Response Factor Cooperates With Progesterone Receptor to Regulate Oviductal Smooth Muscle Gene Expression During Preimplantation Embryo Transport.
- 1Reproductive and Developmental Biology Laboratory, National Institute of Environmental Health Sciences, Research Triangle Park, North Carolina, USA.
- 2Department of Molecular and Cellular Biology, Baylor College of Medicine, Houston, Texas, USA.
Abstract
The oviduct (fallopian tube) houses the site of fertilization and transports embryos to the uterus for on-time implantation. Hormone-regulated contractions of the oviductal smooth muscle (myosalpinx) are thought to participate in this process. Serum response factor (SRF) is a widely expressed transcription factor shown to facilitate smooth muscle development as well as murine oviductal function. Our past work in the uterus has suggested that SRF transcriptional activity is associated with the master pregnancy regulator progesterone receptor (PGR). We therefore hypothesized that SRF and PGR work together to influence oviductal gene expression. Ablation of Srf in the Pgr-expressing cells of the mouse oviduct resulted in extensive transcriptomic consequences. Of 1670 differentially expressed genes in the Srf-deficient oviduct, 306 also contained an SRF binding region within 10 kb of the TSS, including cytoskeletal, contractile, and extracellular matrix genes important for muscle structure and function. Histological analysis confirmed disruption of the smooth muscle layer in the Srf-deficient oviduct, and functional contractility analyses demonstrated that contractile activity was deficient and irregular. Comparative transcriptomic analysis of oviducts deficient in smooth muscle Pgr expression uncovered parallel downregulation of extracellular matrix organization pathways and smooth muscle contraction processes. Integration of SRF and PGR cistromic analyses identified a set of structural and contractile smooth muscle genes co-occupied and co-regulated by SRF and PGR including Myh11, Actg2, and Des. Our findings highlight a prominent regulatory mechanism for SRF and PGR in the oviduct, disruption of which contributes to impaired myosalpinx function and infertility.
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BibTeX
@article{lais2026serum,
title = {{Serum Response Factor Cooperates With Progesterone Receptor to Regulate Oviductal Smooth Muscle Gene Expression During Preimplantation Embryo Transport.}},
author = {Peter F Lais and Ryan M Marquardt and San-Pin Wu and John P Lydon and Francesco J DeMayo},
journal = {FASEB journal : official publication of the Federation of American Societies for Experimental Biology},
year = {2026},
month = oct,
doi = {10.1096/fj.202601362r},
url = {https://europepmc.org/article/MED/42909480},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Serum Response Factor Cooperates With Progesterone Receptor to Regulate Oviductal Smooth Muscle Gene Expression During Preimplantation Embryo Transport.
AU - Peter F Lais
AU - Ryan M Marquardt
AU - San-Pin Wu
AU - John P Lydon
AU - Francesco J DeMayo
PY - 2026
DA - 2026/10/01
JO - FASEB journal : official publication of the Federation of American Societies for Experimental Biology
DO - 10.1096/fj.202601362r
UR - https://europepmc.org/article/MED/42909480
AB - The oviduct (fallopian tube) houses the site of fertilization and transports embryos to the uterus for on-time implantation. Hormone-regulated contractions of the oviductal smooth muscle (myosalpinx) are thought to participate in this process. Serum response factor (SRF) is a widely expressed transcription factor shown to facilitate smooth muscle development as well as murine oviductal function. Our past work in the uterus has suggested that SRF transcriptional activity is associated with the master pregnancy regulator progesterone receptor (PGR). We therefore hypothesized that SRF and PGR work together to influence oviductal gene expression. Ablation of Srf in the Pgr-expressing cells of the mouse oviduct resulted in extensive transcriptomic consequences. Of 1670 differentially expressed genes in the Srf-deficient oviduct, 306 also contained an SRF binding region within 10 kb of the TSS, including cytoskeletal, contractile, and extracellular matrix genes important for muscle structure and function. Histological analysis confirmed disruption of the smooth muscle layer in the Srf-deficient oviduct, and functional contractility analyses demonstrated that contractile activity was deficient and irregular. Comparative transcriptomic analysis of oviducts deficient in smooth muscle Pgr expression uncovered parallel downregulation of extracellular matrix organization pathways and smooth muscle contraction processes. Integration of SRF and PGR cistromic analyses identified a set of structural and contractile smooth muscle genes co-occupied and co-regulated by SRF and PGR including Myh11, Actg2, and Des. Our findings highlight a prominent regulatory mechanism for SRF and PGR in the oviduct, disruption of which contributes to impaired myosalpinx function and infertility.
SN - 0892-6638
SN - 1530-6860
ER -