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Peer-reviewedCC BY-NC 3.0International Journal of Fertility & Sterility

Therapeutic Potential of Bone Marrow-Derived Mesenchymal Stem Cells in A Letrozole-Induced Rat Model of Polycystic Ovary Syndrome

  • Amoura M. Abou-El-Naga1
  • Nada Younes Elarby1
  • Sara El-dusoky2
  • Eman Fayad3
  • Mohamed Ahmed Sobh4
  • Reham A. Ghanem5
5 affiliations
  1. 1Zoology Department, Faculty of Science, Mansoura University, Mansoura, Egypt
  2. 2Medical Experimental Research Center (MERC), Faculty of Medicine, Mansoura University, Mansoura, Egypt
  3. 3Department of Biotechnology, College of Science, Taif University, Taif, Saudi Arabia
  4. 4Zoology Department, Urology and Nephrology Center, Mansoura University, Mansoura, Egypt
  5. 5Oral Biology Department, Faculty of Oral and Dental Medicine, Delta University for Science and Technology, Gamassa, Egypt
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Abstract

Background:: Polycystic ovary syndrome (PCOS) is a prevalent metabolic and reproductive disorder and one of the leading causes of infertility. Bone marrow-derived mesenchymal stem cells (BM-MSCs) possess anti-inflammatory properties and have shown potential in alleviating PCOS manifestations in experimental models. This study aimed to evaluate the therapeutic efficacy of transplanted BM-MSCs and BM-MSC-derived culture media in a letrozole (LTZ)-induced PCOS rat model.

Materials and Methods:: The experimental study was conducted on 50 female Sprague Dawley rats aged (4-6 weeks) and weighing (100-120 g), using a LTZ-induced PCOS model. Animals were randomly assigned to 4 groups, including control, PCOS, BM-MSC-treated, and BM-MSC culture media-treated groups. BM-MSCs were isolated from (bone marrow) and administered via (tail vain) at a dose of (1×106 BM-MSC). Blood samples were collected to measure hormonal and metabolic parameters (insulin, androgen, progesterone, estrogen, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) using [enzyme-linked immunosorbent assay (ELISA) technique). Ovarian tissues were analyzed by for histopathological and immunohistochemical methods to assess [key markers, e.g., follicular development, tumor necrosis factor-alpha (TNF), and vascular endothelial growth factor (VEGF)].

Results:: The PCOS group exhibited hormonal imbalance, metabolic disturbance, and ovarian dysfunction. BM-MSC treatment significantly reduced insulin (P=0.003) and androgen levels (P=0.040), while restoring progesterone (P=0.003), estrogen (P=0.007), FSH (P=0.009), and LH (P=0.001) to near-normal levels. BM-MSCs markedly improved both metabolic and reproductive parameters, effectively restoring fertility in the PCOS model. Treatment with BM-MSC-derived culture media produced partial improvement but did not fully reverse PCOS manifestations.

Conclusion:: BM-MSCs improved ovarian structure and function, ameliorating both metabolic and reproductive dysfunction in the PCOS rat model. BM-MSC transplantation yielded the most favorable outcomes, whereas BM-MSC-derived culture media alone provided incomplete therapeutic benefits.

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Cite

BibTeXRIS
BibTeX
@article{abouelnaga2026therapeutic,
  title = {{Therapeutic Potential of Bone Marrow-Derived Mesenchymal Stem Cells in A Letrozole-Induced Rat Model of Polycystic Ovary Syndrome}},
  author = {Amoura M. Abou-El-Naga and Nada Younes Elarby and Sara El-dusoky and Eman Fayad and Mohamed Ahmed Sobh and Reham A. Ghanem},
  journal = {International Journal of Fertility \& Sterility},
  year = {2026},
  month = oct,
  doi = {10.22074/ijfs.2025.2015395.1568},
  url = {https://doi.org/10.22074/ijfs.2025.2015395.1568},
  note = {CC BY-NC 3.0},
}
RIS
TY  - JOUR
TI  - Therapeutic Potential of Bone Marrow-Derived Mesenchymal Stem Cells in A Letrozole-Induced Rat Model of Polycystic Ovary Syndrome
AU  - Amoura M. Abou-El-Naga
AU  - Nada Younes Elarby
AU  - Sara El-dusoky
AU  - Eman Fayad
AU  - Mohamed Ahmed Sobh
AU  - Reham A. Ghanem
PY  - 2026
DA  - 2026/10/03
JO  - International Journal of Fertility & Sterility
DO  - 10.22074/ijfs.2025.2015395.1568
UR  - https://doi.org/10.22074/ijfs.2025.2015395.1568
AB  - Background:: Polycystic ovary syndrome (PCOS) is a prevalent metabolic and reproductive disorder and one of the leading causes of infertility. Bone marrow-derived mesenchymal stem cells (BM-MSCs) possess anti-inflammatory properties and have shown potential in alleviating PCOS manifestations in experimental models. This study aimed to evaluate the therapeutic efficacy of transplanted BM-MSCs and BM-MSC-derived culture media in a letrozole (LTZ)-induced PCOS rat model.  Materials and Methods:: The experimental study was conducted on 50 female Sprague Dawley rats aged (4-6 weeks) and weighing (100-120 g), using a LTZ-induced PCOS model. Animals were randomly assigned to 4 groups, including control, PCOS, BM-MSC-treated, and BM-MSC culture media-treated groups. BM-MSCs were isolated from (bone marrow) and administered via (tail vain) at a dose of (1×106 BM-MSC). Blood samples were collected to measure hormonal and metabolic parameters (insulin, androgen, progesterone, estrogen, follicle-stimulating hormone (FSH), and luteinizing hormone (LH) using [enzyme-linked immunosorbent assay (ELISA) technique). Ovarian tissues were analyzed by for histopathological and immunohistochemical methods to assess [key markers, e.g., follicular development, tumor necrosis factor-alpha (TNF), and vascular endothelial growth factor (VEGF)].  Results:: The PCOS group exhibited hormonal imbalance, metabolic disturbance, and ovarian dysfunction. BM-MSC treatment significantly reduced insulin (P=0.003) and androgen levels (P=0.040), while restoring progesterone (P=0.003), estrogen (P=0.007), FSH (P=0.009), and LH (P=0.001) to near-normal levels. BM-MSCs markedly improved both metabolic and reproductive parameters, effectively restoring fertility in the PCOS model. Treatment with BM-MSC-derived culture media produced partial improvement but did not fully reverse PCOS manifestations.  Conclusion:: BM-MSCs improved ovarian structure and function, ameliorating both metabolic and reproductive dysfunction in the PCOS rat model. BM-MSC transplantation yielded the most favorable outcomes, whereas BM-MSC-derived culture media alone provided incomplete therapeutic benefits.
SN  - 2008-076X
SN  - 2008-0778
ER  -