O2-tolerant CO2-to-CO reduction by air-sensitive carbon monoxide dehydrogenases in hydrated deep eutectic solvents
Abstract
This work investigates two carbon monoxide dehydrogenase (CODH) enzymes immobilised on functionalised carbon nanotube electrodes and describes their behaviour in two deep eutectic solvents, namely glyceline and ethaline. These CODH bioelectrodes achieve CO2 reduction activities in the mA cm-2 range at 150 mV overpotential and retain their electrocatalytic activity in the presence of up to 14% O2, provided that a reducing potential is maintained. The use of deep eutectic solvents as electrolytes further protects the immobilized enzymes from oxygen damage by preventing direct O2 diffusion towards the electrode surface. This dramatically increases the enzyme half-life from 30 min in water to more than 7 h in hydrated glyceline at the expense of a reduced electrocatalytic activity (TOF of 60 s-1 and maximum current density of 0.45 mA cm-2).
Related
- The Effect of Coke Powder on the Iron Ore Fines Reduction by Carbon Monoxide
- Metal ion's central role in PQQ-dependent alcohol dehydrogenases
- Short-Rotation Coppice Pellets as Biofuels in a Modern Boiler with an Electrostatic Precipitator: Particulate Matter and Carbon Monoxide Emissions
- Many-body machine-learned potential for the hydrated proton at CCSD(T) accuracy with fast analytical gradient
- Beyond Swelling: Electrochemical Doping Tunes the Balance between Ion Compensation and Exchange in a Hydrated Organic Mixed Ionic–Electronic Conductor
BibTeX
@article{olivotto2026o2tolerant,
title = {{O2-tolerant CO2-to-CO reduction by air-sensitive carbon monoxide dehydrogenases in hydrated deep eutectic solvents}},
author = {Léonard Olivotto and Claudio Righetti and Julien Pérard and Moritz F. Kühnel and Christine Cavazza and Alan Le Goff},
journal = {EES Catalysis},
year = {2026},
month = sep,
doi = {10.1039/d6ey00091f},
url = {https://hal.science/hal-05797932v1},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - O2-tolerant CO2-to-CO reduction by air-sensitive carbon monoxide dehydrogenases in hydrated deep eutectic solvents
AU - Léonard Olivotto
AU - Claudio Righetti
AU - Julien Pérard
AU - Moritz F. Kühnel
AU - Christine Cavazza
AU - Alan Le Goff
PY - 2026
DA - 2026/09/14
JO - EES Catalysis
DO - 10.1039/d6ey00091f
UR - https://hal.science/hal-05797932v1
AB - This work investigates two carbon monoxide dehydrogenase (CODH) enzymes immobilised on functionalised carbon nanotube electrodes and describes their behaviour in two deep eutectic solvents, namely glyceline and ethaline. These CODH bioelectrodes achieve CO2 reduction activities in the mA cm-2 range at 150 mV overpotential and retain their electrocatalytic activity in the presence of up to 14% O2, provided that a reducing potential is maintained. The use of deep eutectic solvents as electrolytes further protects the immobilized enzymes from oxygen damage by preventing direct O2 diffusion towards the electrode surface. This dramatically increases the enzyme half-life from 30 min in water to more than 7 h in hydrated glyceline at the expense of a reduced electrocatalytic activity (TOF of 60 s-1 and maximum current density of 0.45 mA cm-2).
SN - 2753-801X
ER -