Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag
Abstract
Muscle stem cells (MuSCs) are a rare stem cell population that provides myofibers with a remarkable capacity to regenerate after tissue injury. Here, we have adapted the Cleavage Under Target and Tagmentation technology to the mapping of the chromatin landscape and transcription factor binding in 50,000 activated MuSCs isolated from injured mouse hindlimb muscles. We have applied this same approach to human CD34 + hematopoietic stem and progenitor cells. This protocol could be adapted to any rare stem cell population. For complete details on the use and execution of this protocol, please refer to Robinson et al. (2021) 24. Robinson, D.C.L. ∙ Ritso, M. ∙ Nelson, G.M. ... Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation Dev. Cell. 2021; 56 :1014-1029 Google Scholar .
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BibTeX
@article{li2026chromatin,
title = {{Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag}},
author = {Yuefeng Li and Kiran Nakka and Thomas Olender and Philippe Gingras-Gelinas and Matthew Man-Kin Wong and Daniel Robinson and Hina Bandukwala and Carmen Palii and Odile Neyret and Marjorie Brand and Alexandre Blais and F Jeffrey Dilworth and Daniel C.L. Robinson and F. Jeffrey Dilworth},
journal = {STAR Protocols},
year = {2026},
month = oct,
doi = {10.1016/j.xpro.2021.100751},
url = {https://hal.science/hal-05794876v1},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Chromatin and transcription factor profiling in rare stem cell populations using CUT&Tag
AU - Yuefeng Li
AU - Kiran Nakka
AU - Thomas Olender
AU - Philippe Gingras-Gelinas
AU - Matthew Man-Kin Wong
AU - Daniel Robinson
AU - Hina Bandukwala
AU - Carmen Palii
AU - Odile Neyret
AU - Marjorie Brand
AU - Alexandre Blais
AU - F Jeffrey Dilworth
AU - Daniel C.L. Robinson
AU - F. Jeffrey Dilworth
PY - 2026
DA - 2026/10/12
JO - STAR Protocols
DO - 10.1016/j.xpro.2021.100751
UR - https://hal.science/hal-05794876v1
AB - Muscle stem cells (MuSCs) are a rare stem cell population that provides myofibers with a remarkable capacity to regenerate after tissue injury. Here, we have adapted the Cleavage Under Target and Tagmentation technology to the mapping of the chromatin landscape and transcription factor binding in 50,000 activated MuSCs isolated from injured mouse hindlimb muscles. We have applied this same approach to human CD34 + hematopoietic stem and progenitor cells. This protocol could be adapted to any rare stem cell population. For complete details on the use and execution of this protocol, please refer to Robinson et al. (2021) 24. Robinson, D.C.L. ∙ Ritso, M. ∙ Nelson, G.M. ... Negative elongation factor regulates muscle progenitor expansion for efficient myofiber repair and stem cell pool repopulation Dev. Cell. 2021; 56 :1014-1029 Google Scholar .
SN - 2666-1667
ER -