Metabolic Responses of Pleurotus djamor to Lignocellulosic Substrate Gradients
- 1Facultad de Ingeniería, Universidad Autónoma de Querétaro, Amazcala, El Marqués 76265, Querétaro, Mexico
- 2Facultad de Química, Universidad Autónoma de Querétaro, Cerro de las Campanas, Santiago de Querétaro 76017, Querétaro, Mexico
Abstract
Pleurotus djamor is cultivated on diverse lignocellulosic residues, but the relationship between progressive substrate formulation, mushroom productivity, and metabolomic responses remains insufficiently characterized. This study evaluated the effects of progressive replacement of barley straw with coconut fiber or coffee husk on mushroom productivity and GC–MS-based metabolomic profiles. Productivity differed markedly among substrate formulations, with intermediate inclusion levels producing the highest yield and biological efficiency. In contrast, PERMANOVA did not detect significant global differences in metabolomic composition across either substrate gradient, and no individual metabolite remained significant after multiple-testing correction. PCA and heatmap analyses revealed exploratory variation in amino acids, carbohydrates, lipids, and related metabolite groups. Pathway analysis identified statistically supported enrichment of alanine, aspartate and glutamate metabolism and cyanoamino acid metabolism in the coffee husk gradient, whereas the remaining pathway associations were exploratory after FDR correction. Overall, the results indicate that substrate composition strongly influenced productivity, while the global metabolomic organization of P. djamor remained comparatively stable, with localized metabolite and pathway patterns that require further validation.
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BibTeX
@article{pachecosanchez2026metabolic,
title = {{Metabolic Responses of Pleurotus djamor to Lignocellulosic Substrate Gradients}},
author = {Humberto Raúl Pacheco-Sánchez and Noemí Alitzel Aguado-Rivera and Carlos Eligio Hernández-Navarrete and Amanda Kim Rico-Chávez and Humberto Aguirre-Becerra and Juan Fernando García-Trejo and Ana Angélica Feregrino-Pérez},
journal = {Molecules (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/molecules31193533},
url = {https://europepmc.org/article/PMC/PMC13667281},
note = {CC BY},
}
RIS
TY - JOUR
TI - Metabolic Responses of Pleurotus djamor to Lignocellulosic Substrate Gradients
AU - Humberto Raúl Pacheco-Sánchez
AU - Noemí Alitzel Aguado-Rivera
AU - Carlos Eligio Hernández-Navarrete
AU - Amanda Kim Rico-Chávez
AU - Humberto Aguirre-Becerra
AU - Juan Fernando García-Trejo
AU - Ana Angélica Feregrino-Pérez
PY - 2026
DA - 2026/10/01
JO - Molecules (Basel, Switzerland)
DO - 10.3390/molecules31193533
UR - https://europepmc.org/article/PMC/PMC13667281
AB - Pleurotus djamor is cultivated on diverse lignocellulosic residues, but the relationship between progressive substrate formulation, mushroom productivity, and metabolomic responses remains insufficiently characterized. This study evaluated the effects of progressive replacement of barley straw with coconut fiber or coffee husk on mushroom productivity and GC–MS-based metabolomic profiles. Productivity differed markedly among substrate formulations, with intermediate inclusion levels producing the highest yield and biological efficiency. In contrast, PERMANOVA did not detect significant global differences in metabolomic composition across either substrate gradient, and no individual metabolite remained significant after multiple-testing correction. PCA and heatmap analyses revealed exploratory variation in amino acids, carbohydrates, lipids, and related metabolite groups. Pathway analysis identified statistically supported enrichment of alanine, aspartate and glutamate metabolism and cyanoamino acid metabolism in the coffee husk gradient, whereas the remaining pathway associations were exploratory after FDR correction. Overall, the results indicate that substrate composition strongly influenced productivity, while the global metabolomic organization of P. djamor remained comparatively stable, with localized metabolite and pathway patterns that require further validation.
SN - 1420-3049
ER -