Increase in 6-pentyl-alpha-pyrone production by Trichoderma spp. in liquid medium using in situ removal by adsorbent resin.
5 affiliations
- 1Department of Pest Management and Conservation, Lincoln University, Lincoln, Canterbury, New Zealand. [email protected].
- 2Department of Pest Management and Conservation, Lincoln University, Lincoln, Canterbury, New Zealand.
- 3Department of Agricultural Sciences, Lincoln University, Lincoln, Canterbury, New Zealand.
- 4Lincoln Agritech, Lincoln, Canterbury, New Zealand.
- 5Department of Pest Management and Conservation, Lincoln University, Lincoln, Canterbury, New Zealand. [email protected].
Abstract
The Trichoderma-produced secondary metabolite 6-pentyl-alpha-pyrone (6-PP) has demonstrated promise for agricultural applications, but cost-effective bioproduction remains limited by product inhibition during fermentation. This study aimed to evaluate the effect of media type on 6-PP production in T. atroviride IMI206040, screen production by nine New Zealand Trichoderma strains, and assess Amberlite XAD-4 in situ product removal in five strains to overcome self-inhibition. T. atroviride IMI206040 was cultured for 5 days in potato dextrose broth (PDB) or minimal media supplemented with ammonium or nitrate, and 6-PP production was assessed by thin-layer chromatography (TLC). The ten Trichoderma strains were cultured for 7 days in PDB and 6-PP production quantified by spectrophotometry. Finally, five strains were cultured in PDB for 14 days with and without Amberlite XAD-4, and 6-PP quantified by spectrophotometry and gas chromatography (GC). In T. atroviride IMI206040, PDB supported the highest 6-PP production, while ammonium-based minimal media repressed production, consistent with nitrogen catabolite repression. Screening revealed up to 14.6-fold variation among strains, with T. gamsii LU771 and T. viridescens LU1369 as the highest producers. Spectrophotometric and GC measurements showed strain-dependent disagreement, indicating GC is more reliable for cross-strain comparisons. Amberlite XAD-4 increased total 6-PP production 3- to 5.2-fold across all five strains, with > 99% of secreted 6-PP sequestered. The highest-producing strains, T. gamsii LU771 and T. atroviride LU132, achieved titres approaching 500 mg/L. These findings support Amberlite XAD-4 as an effective in situ product removal method for increasing 6-PP bioproduction and identifying strains with high intrinsic biosynthetic capacity in the absence of product inhibition.
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BibTeX
@article{maan2026increase,
title = {{Increase in 6-pentyl-alpha-pyrone production by Trichoderma spp. in liquid medium using in situ removal by adsorbent resin.}},
author = {Alex Maan and Eirian Jones and John Hampton and Travis Glare and Artemio Mendoza-Mendoza},
journal = {Natural products and bioprospecting},
year = {2026},
month = oct,
doi = {10.1007/s13659-026-00650-y},
url = {https://europepmc.org/article/MED/42895689},
note = {CC BY},
}
RIS
TY - JOUR
TI - Increase in 6-pentyl-alpha-pyrone production by Trichoderma spp. in liquid medium using in situ removal by adsorbent resin.
AU - Alex Maan
AU - Eirian Jones
AU - John Hampton
AU - Travis Glare
AU - Artemio Mendoza-Mendoza
PY - 2026
DA - 2026/10/19
JO - Natural products and bioprospecting
DO - 10.1007/s13659-026-00650-y
UR - https://europepmc.org/article/MED/42895689
AB - The Trichoderma-produced secondary metabolite 6-pentyl-alpha-pyrone (6-PP) has demonstrated promise for agricultural applications, but cost-effective bioproduction remains limited by product inhibition during fermentation. This study aimed to evaluate the effect of media type on 6-PP production in T. atroviride IMI206040, screen production by nine New Zealand Trichoderma strains, and assess Amberlite XAD-4 in situ product removal in five strains to overcome self-inhibition. T. atroviride IMI206040 was cultured for 5 days in potato dextrose broth (PDB) or minimal media supplemented with ammonium or nitrate, and 6-PP production was assessed by thin-layer chromatography (TLC). The ten Trichoderma strains were cultured for 7 days in PDB and 6-PP production quantified by spectrophotometry. Finally, five strains were cultured in PDB for 14 days with and without Amberlite XAD-4, and 6-PP quantified by spectrophotometry and gas chromatography (GC). In T. atroviride IMI206040, PDB supported the highest 6-PP production, while ammonium-based minimal media repressed production, consistent with nitrogen catabolite repression. Screening revealed up to 14.6-fold variation among strains, with T. gamsii LU771 and T. viridescens LU1369 as the highest producers. Spectrophotometric and GC measurements showed strain-dependent disagreement, indicating GC is more reliable for cross-strain comparisons. Amberlite XAD-4 increased total 6-PP production 3- to 5.2-fold across all five strains, with > 99% of secreted 6-PP sequestered. The highest-producing strains, T. gamsii LU771 and T. atroviride LU132, achieved titres approaching 500 mg/L. These findings support Amberlite XAD-4 as an effective in situ product removal method for increasing 6-PP bioproduction and identifying strains with high intrinsic biosynthetic capacity in the absence of product inhibition.
SN - 2192-2195
SN - 2192-2209
ER -