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Peer-reviewedCC BY-NC-NDJournal of Agricultural and Food Chemistry

Overexpression of Po-cat1 Influences the MAPK Signaling Pathway by Inhibiting H2O2 Production and PoPTP1 Transcription, Thereby Affecting the Fruiting Development of Pleurotus ostreatus

  • Qi He1,2,3
  • Chenyang Huang1
  • Lijiao Zhang1
  • Fangjie Yao3,4
  • Mengran Zhao1
4 affiliations
  1. 1State Key Laboratory of Efficient Utilization of Arable Land in China/Key Laboratory of Microbial Resources Collection and Preservation, Ministry of Agriculture and Rural Affairs, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing 100081, China
  2. 2Jiaxing Academy of Agricultural Science, Jiaxing 314016, China
  3. 3Engineering Research Centre of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, Changchun 130118, China
  4. 4College of Horticulture, Jilin Agricultural University, Changchun 130118, China
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Abstract

Fruiting body development critically affects yield and cultivation efficiency in edible mushrooms. Although catalase (CAT) is essential for development across various organisms, its function in edible mushrooms remains poorly understood. This study investigated the function of catalase in Pleurotus ostreatus fruiting body development and identified Po-cat1 as the key gene. Increased Po-cat1 transcription raised total CAT activity and lowered intracellular H2O2 level. H2O2 acts as a positive signal for fruiting, and Po-CAT1 negatively regulates this signal by reducing H2O2, thereby inhibiting development. Transcriptome analysis revealed that the change of Po-cat1 expression altered gene expression profiles in the MAPK pathway and affected PoPTP1 transcription, possibly via H2O2. PoPTP1 promoted fruiting body development. Its overexpression reduces protein phosphorylation levels in the MAPK pathway in mycelium and fruiting body stages but increases them in primordium stage. These findings demonstrate the primary role of Po-CAT1 in P. ostreatus fruiting body development.

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Cite

BibTeXRIS
BibTeX
@article{he2026overexpression,
  title = {{Overexpression of Po-cat1 Influences the MAPK Signaling Pathway by Inhibiting H2O2 Production and PoPTP1 Transcription, Thereby Affecting the Fruiting Development of Pleurotus ostreatus}},
  author = {Qi He and Chenyang Huang and Lijiao Zhang and Fangjie Yao and Mengran Zhao},
  journal = {Journal of Agricultural and Food Chemistry},
  year = {2026},
  month = oct,
  doi = {10.1021/acs.jafc.6c03807},
  url = {https://doi.org/10.1021/acs.jafc.6c03807},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - Overexpression of Po-cat1 Influences the MAPK Signaling Pathway by Inhibiting H2O2 Production and PoPTP1 Transcription, Thereby Affecting the Fruiting Development of Pleurotus ostreatus
AU  - Qi He
AU  - Chenyang Huang
AU  - Lijiao Zhang
AU  - Fangjie Yao
AU  - Mengran Zhao
PY  - 2026
DA  - 2026/10/21
JO  - Journal of Agricultural and Food Chemistry
DO  - 10.1021/acs.jafc.6c03807
UR  - https://doi.org/10.1021/acs.jafc.6c03807
AB  - Fruiting body development critically affects yield and cultivation efficiency in edible mushrooms. Although catalase (CAT) is essential for development across various organisms, its function in edible mushrooms remains poorly understood. This study investigated the function of catalase in Pleurotus ostreatus fruiting body development and identified Po-cat1 as the key gene. Increased Po-cat1 transcription raised total CAT activity and lowered intracellular H2O2 level. H2O2 acts as a positive signal for fruiting, and Po-CAT1 negatively regulates this signal by reducing H2O2, thereby inhibiting development. Transcriptome analysis revealed that the change of Po-cat1 expression altered gene expression profiles in the MAPK pathway and affected PoPTP1 transcription, possibly via H2O2. PoPTP1 promoted fruiting body development. Its overexpression reduces protein phosphorylation levels in the MAPK pathway in mycelium and fruiting body stages but increases them in primordium stage. These findings demonstrate the primary role of Po-CAT1 in P. ostreatus fruiting body development.
SN  - 0021-8561
SN  - 1520-5118
ER  -