Skip to content
DraconiticResearch
Search Sources API
201,683 studies31 sourcesUpdated 50 min ago
Peer-reviewedCC BYJournal of agricultural and food chemistry

Ligand-Baited Proteomics Reveals Vesicle Trafficking Proteins as Putative Site of Action of Indaziflam.

  • Mohit Mahey1,2
  • Peter Knut Lundquist3,2
  • Eric Lloyd Patterson1
  1. 1Department of Plant, Soil, and Microbial Sciences, Michigan State University, East Lansing, Michigan48824, United States.
  2. 2Plant Resilience Institute, Michigan State University, East Lansing, Michigan48824, United States.
  3. 3Department of Biochemistry & Molecular Biology, Michigan State University, East Lansing, Michigan48824, United States.
Read full textPDFDOICite

Abstract

Indaziflam is a potent herbicide that has become an important tool for pre-emergence weed control. Although it is classified as a cellulose biosynthesis inhibitor, its precise site of action remains unresolved. Here, we show that indaziflam preferentially affects the root elongation zone, where cells exhibit swelling and frequent rupture. We performed ligand-baited coprecipitation assays using root tissue lysates from Arabidopsis thaliana and Oryza sativa. Proteomic analysis revealed that the highly enriched proteins were associated with vesicular trafficking, including multiple coatomer subunits, while no cellulose synthase components were detected. Live-cell imaging using FM4-64 dye demonstrated that indaziflam does not cause a complete inhibition of clathrin-mediated endocytosis. AlphaFold3-based protein-ligand modeling further identified relatively stable predicted indaziflam poses with select trafficking-associated proteins, including coatomer subunit β. Collectively, our results support a model in which indaziflam indirectly disrupts cellulose biosynthesis by interfering with intracellular trafficking processes required for proper cellulose synthase complex homeostasis.

Related

  1. Putative probiotics decrease cell viability and enhance chemotherapy effectiveness in human cancer cells: role of butyrate and secreted proteins
    S. Doublier, S. Cirrincione, R. Scardaci +7
    Microbiological ResearchPeer-reviewedLicence not statedFull text19 citedGeneral biologyHAL
  2. Systematic Analysis of GARP- and EARP-Mediated Trafficking Pathways Using Lysosomal Proteomics
    B. Sivalingam, J. Altuzar, S. Walter +3
    bioRxivPreprintCC BYFull textCell biologybioRxiv
  3. High-throughput Eukaryotic Screening of Putative Anti-CRISPR Proteins against SpyCas9 and SauCas9
    D. Zheng, T. R. Grieve, E. Fidacaro +2
    bioRxivPreprintCC0Full textGenetics & genomicsbioRxivPMC
  4. Proteomics to uncover new actors in the antifungal action of metformin. Impact on virulence traits, oxidative stress, and essential proteins from Candida albicans.
    V. Mascaraque, C. Ramos, C. Navas +3
    bioRxivPreprintAll rights reservedFull textMicrobiologybioRxiv
  5. Protocol for Single-Cell Patch-Proteomics: identify specific proteins associated with electrophysiological properties from individual neurons
    J. C. Althaus, Y. Cao, Y. Song +5
    bioRxivPreprintCC BY-NC-NDFull textNeurosciencebioRxiv

Cite

BibTeXRIS
BibTeX
@article{mahey2026ligandbaited,
  title = {{Ligand-Baited Proteomics Reveals Vesicle Trafficking Proteins as Putative Site of Action of Indaziflam.}},
  author = {Mohit Mahey and Peter Knut Lundquist and Eric Lloyd Patterson},
  journal = {Journal of agricultural and food chemistry},
  year = {2026},
  month = oct,
  doi = {10.1021/acs.jafc.6c07779},
  url = {https://europepmc.org/article/MED/42904991},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Ligand-Baited Proteomics Reveals Vesicle Trafficking Proteins as Putative Site of Action of Indaziflam.
AU  - Mohit Mahey
AU  - Peter Knut Lundquist
AU  - Eric Lloyd Patterson
PY  - 2026
DA  - 2026/10/01
JO  - Journal of agricultural and food chemistry
DO  - 10.1021/acs.jafc.6c07779
UR  - https://europepmc.org/article/MED/42904991
AB  - Indaziflam is a potent herbicide that has become an important tool for pre-emergence weed control. Although it is classified as a cellulose biosynthesis inhibitor, its precise site of action remains unresolved. Here, we show that indaziflam preferentially affects the root elongation zone, where cells exhibit swelling and frequent rupture. We performed ligand-baited coprecipitation assays using root tissue lysates from Arabidopsis thaliana and Oryza sativa. Proteomic analysis revealed that the highly enriched proteins were associated with vesicular trafficking, including multiple coatomer subunits, while no cellulose synthase components were detected. Live-cell imaging using FM4-64 dye demonstrated that indaziflam does not cause a complete inhibition of clathrin-mediated endocytosis. AlphaFold3-based protein-ligand modeling further identified relatively stable predicted indaziflam poses with select trafficking-associated proteins, including coatomer subunit β. Collectively, our results support a model in which indaziflam indirectly disrupts cellulose biosynthesis by interfering with intracellular trafficking processes required for proper cellulose synthase complex homeostasis.
SN  - 0021-8561
SN  - 1520-5118
ER  -