Full-field fluorescence computed tomography (F3CT) using a calibrated virtual cone-beam pinhole geometry
Abstract
We present F3CT, a synchrotron-based hyperspectral full-field fluorescence computed tomography technique that avoids raster scanning by combining a pinhole aperture with an energy-resolving 2D detector. A virtual cone-beam model and two-stage calibration-reconstruction workflow enable 3D elemental mapping under full-field illumination. The method is demonstrated on biological and geological specimens, resolving silver-stain distributions in zebrafish tissue and high-energy fluorescence signatures in rock cores. Phase-contrast tomograms acquired sequentially under the same experimental geometry provide co-registered structural context. F3CT provides a high-throughput route to 3D XRF imaging and establishes a foundation for correlated structural and chemical tomography, with potential for future in-situ and operando implementations.
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BibTeX
@misc{zillhardt2026fullfield,
title = {{Full-field fluorescence computed tomography (F3CT) using a calibrated virtual cone-beam pinhole geometry}},
author = {Thomas Zillhardt and Yunhui Chen and Alexander Rack and Matthew Veale and Matt Wilson and Philip J. Withers and Nicola Viganò},
howpublished = {arXiv},
year = {2026},
month = aug,
doi = {10.48550/arxiv.2608.28275},
eprint = {2608.28275},
archivePrefix = {arXiv},
url = {https://arxiv.org/abs/2608.28275},
note = {CC BY 4.0},
}
RIS
TY - UNPB
TI - Full-field fluorescence computed tomography (F3CT) using a calibrated virtual cone-beam pinhole geometry
AU - Thomas Zillhardt
AU - Yunhui Chen
AU - Alexander Rack
AU - Matthew Veale
AU - Matt Wilson
AU - Philip J. Withers
AU - Nicola Viganò
PY - 2026
DA - 2026/08/28
PB - arXiv
DO - 10.48550/arxiv.2608.28275
UR - https://arxiv.org/abs/2608.28275
AB - We present F3CT, a synchrotron-based hyperspectral full-field fluorescence computed tomography technique that avoids raster scanning by combining a pinhole aperture with an energy-resolving 2D detector. A virtual cone-beam model and two-stage calibration-reconstruction workflow enable 3D elemental mapping under full-field illumination. The method is demonstrated on biological and geological specimens, resolving silver-stain distributions in zebrafish tissue and high-energy fluorescence signatures in rock cores. Phase-contrast tomograms acquired sequentially under the same experimental geometry provide co-registered structural context. F3CT provides a high-throughput route to 3D XRF imaging and establishes a foundation for correlated structural and chemical tomography, with potential for future in-situ and operando implementations.
ER -