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Peer-reviewedCC BY 4.0Life Science Alliance

Esc2 and Rad57 contribute to RNA polymerase II distribution at highly transcribed genes

  • Hiroshi Masumoto1,2
  • Hideki Muto1,2
  • Yohei Kurosaki3
  • Hironori Niki4
4 affiliations
  1. 1Biomedical Research Support Center (BRSC), Nagasaki University School of Medicine, Nagasaki, Japan
  2. 2Research Center for Advanced Genomics, Nagasaki University School of Medicine, Nagasaki, Japan
  3. 3National Research Center for the Control and Prevention of Infectious Diseases, Nagasaki University
  4. 4Microbial Physiology Laboratory, Department of Gene Function and Phenomics, National Institute of Genetics, Shizuoka, Japan
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Abstract

Transcription by RNA polymerase II (Pol II) must be tightly regulated to maintain gene expression homeostasis, yet how Pol II occupancy is shaped at highly active genes remains incompletely understood. Here, we examine the relationship between the homologous recombination–related factors Esc2 and Rad57 and Pol II distribution in budding yeast. Genome-wide analyses reveal that Esc2 and Rad57 preferentially associate with the gene bodies of highly transcribed housekeeping genes and are linked to a chromatin context that also includes the protein phosphatase Sit4 and its cofactor Rrd1. Loss of Esc2 and Rad57 is accompanied by increased Pol II occupancy within gene bodies, whereas the effect on steady-state RNA abundance is selective rather than uniform. Under the tested anchor-away conditions, acute Rad57 depletion in an esc2 Δ background did not fully recapitulate the broader transcriptome imbalance and impaired growth observed after chronic Esc2/Rad57 loss. Together, our findings reveal an association between recombination-related factors and gene-body Pol II occupancy and suggest that Esc2/Rad57 loss is linked to transcription-associated chromatin changes in yeast.

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Cite

BibTeXRIS
BibTeX
@article{masumoto2026esc2,
  title = {{Esc2 and Rad57 contribute to RNA polymerase II distribution at highly transcribed genes}},
  author = {Hiroshi Masumoto and Hideki Muto and Yohei Kurosaki and Hironori Niki},
  journal = {Life Science Alliance},
  year = {2026},
  month = oct,
  doi = {10.26508/lsa.202603673},
  url = {https://www.life-science-alliance.org/lookup/doi/10.26508/lsa.202603673},
  note = {CC BY 4.0},
}
RIS
TY  - JOUR
TI  - Esc2 and Rad57 contribute to RNA polymerase II distribution at highly transcribed genes
AU  - Hiroshi Masumoto
AU  - Hideki Muto
AU  - Yohei Kurosaki
AU  - Hironori Niki
PY  - 2026
DA  - 2026/10/12
JO  - Life Science Alliance
DO  - 10.26508/lsa.202603673
UR  - https://www.life-science-alliance.org/lookup/doi/10.26508/lsa.202603673
AB  - Transcription by RNA polymerase II (Pol II) must be tightly regulated to maintain gene expression homeostasis, yet how Pol II occupancy is shaped at highly active genes remains incompletely understood. Here, we examine the relationship between the homologous recombination–related factors Esc2 and Rad57 and Pol II distribution in budding yeast. Genome-wide analyses reveal that Esc2 and Rad57 preferentially associate with the gene bodies of highly transcribed housekeeping genes and are linked to a chromatin context that also includes the protein phosphatase Sit4 and its cofactor Rrd1. Loss of Esc2 and Rad57 is accompanied by increased Pol II occupancy within gene bodies, whereas the effect on steady-state RNA abundance is selective rather than uniform. Under the tested anchor-away conditions, acute Rad57 depletion in an esc2 Δ background did not fully recapitulate the broader transcriptome imbalance and impaired growth observed after chronic Esc2/Rad57 loss. Together, our findings reveal an association between recombination-related factors and gene-body Pol II occupancy and suggest that Esc2/Rad57 loss is linked to transcription-associated chromatin changes in yeast.
SN  - 2575-1077
ER  -