Enzymatic attachment of antigens to probe immune modulation by skin commensals

Abstract

The skin colonist Staphylococcus epidermidis induces potent T and B cell responses, which can be redirected against antigens of interest by genetic engineering. However, since many taxa in the microbiome are genetically intractable, the utility of this method is limited. Here, we use recombinant sortase to conjugate antigens to the surface of a wide range of Gram-positive bacterial species, and we show that this non-genetic method can be used to generate protective immunity in vivo.

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BibTeX RIS

BibTeX

@misc{bousbaine2026enzymatic,
  title = {{Enzymatic attachment of antigens to probe immune modulation by skin commensals}},
  author = {D. Bousbaine and P. V. Lalgudi and K. D. Bauman and E. Tsang and T. T. D. Nguyen and A. James and M. Chiang and A. Zhao and M. Terasaki and J. M. Swenson and A. Trinh and M. A. Fischbach},
  howpublished = {bioRxiv},
  year = {2026},
  month = oct,
  doi = {10.64898/2026.10.31.763323},
  url = {https://www.biorxiv.org/content/10.64898/2026.10.31.763323v1},
  note = {CC BY},
}

RIS

TY  - UNPB
TI  - Enzymatic attachment of antigens to probe immune modulation by skin commensals
AU  - D. Bousbaine
AU  - P. V. Lalgudi
AU  - K. D. Bauman
AU  - E. Tsang
AU  - T. T. D. Nguyen
AU  - A. James
AU  - M. Chiang
AU  - A. Zhao
AU  - M. Terasaki
AU  - J. M. Swenson
AU  - A. Trinh
AU  - M. A. Fischbach
PY  - 2026
DA  - 2026/10/31
PB  - bioRxiv
DO  - 10.64898/2026.10.31.763323
UR  - https://www.biorxiv.org/content/10.64898/2026.10.31.763323v1
AB  - The skin colonist Staphylococcus epidermidis induces potent T and B cell responses, which can be redirected against antigens of interest by genetic engineering. However, since many taxa in the microbiome are genetically intractable, the utility of this method is limited. Here, we use recombinant sortase to conjugate antigens to the surface of a wide range of Gram-positive bacterial species, and we show that this non-genetic method can be used to generate protective immunity in vivo.
ER  -

Abstract

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