Activatable 19F NMR probes from drug scaffolds for assessing myeloperoxidase-associated inflammation
- 1State Key Laboratory of Microbial Technology, Department of Radiology, Nanjing Drum Tower Hospital, Jiangsu Collaborative Innovation Center of Biomedical Functional Materials, School of Chemistry and Materials Science, Nanjing Normal University Nanjing 210023 China
- 2Department of Radiology, Nanjing Drum Tower Hospital, Affiliated Hospital of Medical School, Nanjing University Nanjing 210008 China
Abstract
Myeloperoxidase (MPO)-derived hypochlorous acid (HClO) is a key oxidant in inflammatory diseases such as sepsis and neuroinflammation. Selective detection of MPO-derived HClO in complex biological matrices remains challenging. 19F NMR spectroscopy offers zero-background, environment-sensitive chemical shifts and easy quantification. Herein, we report a series of drug-derived, nitrone-based 19F NMR probes (19FP-1 to 19FP-14) that respond specifically to HClO via nitrone oxidation, producing a distinct 19F chemical shift change. Systematic screening identified 19FP-10 as the optimal probe, exhibiting a chemical shift change of >2 ppm, high selectivity over other reactive oxygen species, and a linear concentration-dependent response to HClO. Importantly, 19FP-10 enables effective detection of HClO in mouse plasma from a sepsis model and discriminates between inflamed and healthy brain tissue in a neuroinflammation model. Additionally, 19FP-10 protects cells from oxidative stress and exhibits good in vivo biosafety, underscoring its translational potential. This work successfully establishes a class of activatable 19F NMR probes based on drug scaffolds, featuring both low toxicity and high selectivity, providing a clinically translatable molecular tool for assessing MPO-associated inflammation.
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BibTeX
@article{luo2026activatable,
title = {{Activatable 19F NMR probes from drug scaffolds for assessing myeloperoxidase-associated inflammation}},
author = {Xiangjie Luo and Xinran Ji and Zhuoying Chen and Haoyu Wu and Han Cheng and Bing Zhang and Yong Qian},
journal = {Chemical Science},
year = {2026},
month = sep,
doi = {10.1039/d6sc06063c},
url = {https://doi.org/10.1039/d6sc06063c},
note = {CC BY-NC 3.0},
}
RIS
TY - JOUR
TI - Activatable 19F NMR probes from drug scaffolds for assessing myeloperoxidase-associated inflammation
AU - Xiangjie Luo
AU - Xinran Ji
AU - Zhuoying Chen
AU - Haoyu Wu
AU - Han Cheng
AU - Bing Zhang
AU - Yong Qian
PY - 2026
DA - 2026/09/22
JO - Chemical Science
DO - 10.1039/d6sc06063c
UR - https://doi.org/10.1039/d6sc06063c
AB - Myeloperoxidase (MPO)-derived hypochlorous acid (HClO) is a key oxidant in inflammatory diseases such as sepsis and neuroinflammation. Selective detection of MPO-derived HClO in complex biological matrices remains challenging. 19F NMR spectroscopy offers zero-background, environment-sensitive chemical shifts and easy quantification. Herein, we report a series of drug-derived, nitrone-based 19F NMR probes (19FP-1 to 19FP-14) that respond specifically to HClO via nitrone oxidation, producing a distinct 19F chemical shift change. Systematic screening identified 19FP-10 as the optimal probe, exhibiting a chemical shift change of >2 ppm, high selectivity over other reactive oxygen species, and a linear concentration-dependent response to HClO. Importantly, 19FP-10 enables effective detection of HClO in mouse plasma from a sepsis model and discriminates between inflamed and healthy brain tissue in a neuroinflammation model. Additionally, 19FP-10 protects cells from oxidative stress and exhibits good in vivo biosafety, underscoring its translational potential. This work successfully establishes a class of activatable 19F NMR probes based on drug scaffolds, featuring both low toxicity and high selectivity, providing a clinically translatable molecular tool for assessing MPO-associated inflammation.
SN - 2041-6520
SN - 2041-6539
ER -