Growth-Associated Reorganization of Gill and Skin-Mucus Microbiota in Cultured Decapterus maruadsi: Contrasting Occurrence-Abundance Patterns.
- 1College of Marine Sciences, Ningde Normal University, Ningde 352100, China.
- 2Engineering Research Center of Mindong Aquatic Product Deep-Processing, Ningde 352100, China.
- 3School of Medicine, Fuzhou University, Fuzhou 350108, China.
Abstract
External mucosal microbiota form important interfaces between fish and the aquatic environment, yet their variation during host growth remains poorly understood. We characterized the gill and skin-mucus microbiota of cultured Decapterus maruadsi across three successive body-size stages (4.98-6.81 g, 16.67-19.63 g, and 27.30-28.66 g) using 16S rRNA gene amplicon sequencing. Fish were sampled from the same cohort maintained in the same culture system, and two supplied-seawater samples were included as environmental references. Factorial multivariate analysis indicated significant effects of mucosal tissue, sampling stage, and their interaction on community composition. Broad taxonomic structure remained dominated by Pseudomonadota, whereas extensive differentiation occurred at finer taxonomic resolutions. Skin-mucus richness decreased across successive sampling stages, whereas no corresponding alpha-diversity difference was detected in the gill. Host-wide prevalence analysis revealed contrasting changes in the abundance contribution of recurrent ASVs between the two mucosal compartments; however, tissue-specific sensitivity analyses showed that occurrence categories depended partly on the sample set used to define prevalence. The same dominant Limimaricola ASV occurred at high abundance in one supplied-seawater sample and in middle-stage skin mucus, indicating environmental microbial input as a plausible contributor to the observed composition. Overall, this study provides a descriptive baseline for stage-associated variation in the external mucosal microbiota of one cultured D. maruadsi cohort and highlights distinct responses of gill and skin-mucus communities without attributing these changes solely to host growth.
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BibTeX
@article{wu2026growthassociated,
title = {{Growth-Associated Reorganization of Gill and Skin-Mucus Microbiota in Cultured Decapterus maruadsi: Contrasting Occurrence-Abundance Patterns.}},
author = {Xiaoping Wu and Fangyu Yuan and Linkai Chen and Zhaokai Yu and Hancheng Hong and Ding Li and Weiqing Huang},
journal = {Veterinary sciences},
year = {2026},
month = oct,
doi = {10.3390/vetsci13101073},
url = {https://europepmc.org/article/MED/42944168},
note = {CC BY},
}
RIS
TY - JOUR
TI - Growth-Associated Reorganization of Gill and Skin-Mucus Microbiota in Cultured Decapterus maruadsi: Contrasting Occurrence-Abundance Patterns.
AU - Xiaoping Wu
AU - Fangyu Yuan
AU - Linkai Chen
AU - Zhaokai Yu
AU - Hancheng Hong
AU - Ding Li
AU - Weiqing Huang
PY - 2026
DA - 2026/10/10
JO - Veterinary sciences
DO - 10.3390/vetsci13101073
UR - https://europepmc.org/article/MED/42944168
AB - External mucosal microbiota form important interfaces between fish and the aquatic environment, yet their variation during host growth remains poorly understood. We characterized the gill and skin-mucus microbiota of cultured Decapterus maruadsi across three successive body-size stages (4.98-6.81 g, 16.67-19.63 g, and 27.30-28.66 g) using 16S rRNA gene amplicon sequencing. Fish were sampled from the same cohort maintained in the same culture system, and two supplied-seawater samples were included as environmental references. Factorial multivariate analysis indicated significant effects of mucosal tissue, sampling stage, and their interaction on community composition. Broad taxonomic structure remained dominated by Pseudomonadota, whereas extensive differentiation occurred at finer taxonomic resolutions. Skin-mucus richness decreased across successive sampling stages, whereas no corresponding alpha-diversity difference was detected in the gill. Host-wide prevalence analysis revealed contrasting changes in the abundance contribution of recurrent ASVs between the two mucosal compartments; however, tissue-specific sensitivity analyses showed that occurrence categories depended partly on the sample set used to define prevalence. The same dominant Limimaricola ASV occurred at high abundance in one supplied-seawater sample and in middle-stage skin mucus, indicating environmental microbial input as a plausible contributor to the observed composition. Overall, this study provides a descriptive baseline for stage-associated variation in the external mucosal microbiota of one cultured D. maruadsi cohort and highlights distinct responses of gill and skin-mucus communities without attributing these changes solely to host growth.
SN - 2306-7381
ER -