A Simplified and Solvent-Reduced HPLC-UV/Vis Method for the Simultaneous Determination of Major Marker Compounds in Rhodiola rosea L.
4 affiliations
- 1Department of Bioengineering, University of Applied Sciences Weihenstephan-Triesdorf, Am Hofgarten 10, 85354 Freising, Germany
- 2Institute of Horticulture, University of Applied Sciences Weihenstephan-Triesdorf, Am Staudengarten 8, 85354 Freising, Germany
- 3Digital Agriculture, HEF World Agricultural Systems Center, Department of Life Science Engineering, TUM School of Life Sciences, Technical University of Munich, 85354 Freising, Germany
- 4Institute of Food Technology, University of Applied Sciences Weihenstephan-Triesdorf, Am Staudengarten 11, 85354 Freising, Germany
Abstract
Rhodiola rosea L. is traditionally used to relieve stress-related symptoms and has been investigated for its potential effects on depression and anxiety. The present study developed and validated a solvent-reduced HPLC-UV/Vis method for the determination of characteristic secondary metabolites in R. rosea. To minimize solvent consumption and eliminate methanol from sample preparation, extraction with 40% (v/v) ethanol was combined with a simplified chromatographic protocol. Extracts were analyzed by HPLC-UV/Vis for the target analytes salidroside, tyrosol, rosarin, rosavin, and rosin. Chromatographic separation was achieved within 35 min using a water–acetonitrile gradient at a flow rate of 0.8 mL min−1. Compared with the reference method described by Ganzera et al., the optimized protocol reduced the total analysis time from 44 to 35 min and eliminated the post-run methanol washing step. Method validation yielded analyte-specific recoveries ranging from 102 ± 3% to 114 ± 4% and relative standard deviations from 0.5% to 8.0%. The limits of quantification were 0.497, 0.302, 0.477, 0.148, and 0.189 µg mL−1 for salidroside, tyrosol, rosarin, rosavin, and rosin, respectively. Quantification was performed by external standard calibration. The developed method offers a simplified, solvent-reduced approach for the quantitative determination of major R. rosea marker compounds, thereby reducing both analysis time and solvent consumption.
Related
- Effect of Rhodiola rosea L. Extract on Alzheimer's Disease-like Symptoms in Caenorhabditis elegans.
- Antioxidant but not ergogenic or altitude-protective: a meta-analysis of Rhodiola species in healthy adults.
- Effects of Rhodiola rosea and caffeine, alone and in combination, on Wingate performance and physiological stress responses in soccer players
- A Simplified Density Gradient–Free Method for Isolating Functional Symbiosomes From Legume Nodules
- Development of a simplified intensified fed-batch CHO cell culture processbased on a commercial single-feed strategy
BibTeX
@article{stabl2026simplified,
title = {{A Simplified and Solvent-Reduced HPLC-UV/Vis Method for the Simultaneous Determination of Major Marker Compounds in Rhodiola rosea L.}},
author = {Alexandra Stabl and Katalin Reich and Ivonne Wittmann and Sabine Wittmann and Özlem Özmutlu Karslioglu and Sabine Grüner},
journal = {Molecules},
year = {2026},
month = oct,
doi = {10.3390/molecules31203734},
url = {https://www.mdpi.com/1420-3049/31/20/3734},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - A Simplified and Solvent-Reduced HPLC-UV/Vis Method for the Simultaneous Determination of Major Marker Compounds in Rhodiola rosea L.
AU - Alexandra Stabl
AU - Katalin Reich
AU - Ivonne Wittmann
AU - Sabine Wittmann
AU - Özlem Özmutlu Karslioglu
AU - Sabine Grüner
PY - 2026
DA - 2026/10/01
JO - Molecules
DO - 10.3390/molecules31203734
UR - https://www.mdpi.com/1420-3049/31/20/3734
AB - Rhodiola rosea L. is traditionally used to relieve stress-related symptoms and has been investigated for its potential effects on depression and anxiety. The present study developed and validated a solvent-reduced HPLC-UV/Vis method for the determination of characteristic secondary metabolites in R. rosea. To minimize solvent consumption and eliminate methanol from sample preparation, extraction with 40% (v/v) ethanol was combined with a simplified chromatographic protocol. Extracts were analyzed by HPLC-UV/Vis for the target analytes salidroside, tyrosol, rosarin, rosavin, and rosin. Chromatographic separation was achieved within 35 min using a water–acetonitrile gradient at a flow rate of 0.8 mL min−1. Compared with the reference method described by Ganzera et al., the optimized protocol reduced the total analysis time from 44 to 35 min and eliminated the post-run methanol washing step. Method validation yielded analyte-specific recoveries ranging from 102 ± 3% to 114 ± 4% and relative standard deviations from 0.5% to 8.0%. The limits of quantification were 0.497, 0.302, 0.477, 0.148, and 0.189 µg mL−1 for salidroside, tyrosol, rosarin, rosavin, and rosin, respectively. Quantification was performed by external standard calibration. The developed method offers a simplified, solvent-reduced approach for the quantitative determination of major R. rosea marker compounds, thereby reducing both analysis time and solvent consumption.
SN - 1420-3049
ER -