Enhancer control of promoter activity and variability via frequency modulation of clustered transcriptional bursts
- 1Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland
- 2University of Basel, Basel, Switzerland
- 3Friedrich Miescher Institute for Biomedical Research, Basel, Switzerland. [email protected]
Abstract
Gene expression in mammalian cells is controlled by enhancers that are often dispersed across large cis-regulatory landscapes around a promoter. How enhancers determine transcription of their target genes and how this depends on their relative position within a cis-regulatory landscape remain unclear. Here we use live-cell imaging to track the activity of a promoter under the control of the same enhancer, but inserted at different positions across a simplified regulatory landscape with minimal complexity. Combined with mathematical modeling, this reveals that RNA production from the promoter occurs in clusters of transcriptional bursts, with enhancer position controlling the frequency at which clusters appear. This results in bursts being more frequent and occurring more uniformly across cells when the enhancer is genomically close to the promoter than when it is located at a large genomic distance. Mathematical modeling further indicates that the enhancer modulates the promoter's ability to transition from its basal transcriptional state to a regime in which clusters of bursts become more frequent. Our results reveal unexplored modes of mammalian promoter operation and show that enhancer position within a cis-regulatory landscape critically controls the timing and variability of transcriptional output in single cells.
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BibTeX
@article{tunnermann2026enhancer,
title = {{Enhancer control of promoter activity and variability via frequency modulation of clustered transcriptional bursts}},
author = {Jana Tünnermann and Grégory Roth and Julie Cramard and Luca Giorgetti},
journal = {Nature Genetics},
year = {2026},
month = jul,
doi = {10.1038/s41588-026-02676-x},
url = {https://doi.org/10.1038/s41588-026-02676-x},
note = {CC BY},
}
RIS
TY - JOUR
TI - Enhancer control of promoter activity and variability via frequency modulation of clustered transcriptional bursts
AU - Jana Tünnermann
AU - Grégory Roth
AU - Julie Cramard
AU - Luca Giorgetti
PY - 2026
DA - 2026/07/14
JO - Nature Genetics
DO - 10.1038/s41588-026-02676-x
UR - https://doi.org/10.1038/s41588-026-02676-x
AB - Gene expression in mammalian cells is controlled by enhancers that are often dispersed across large cis-regulatory landscapes around a promoter. How enhancers determine transcription of their target genes and how this depends on their relative position within a cis-regulatory landscape remain unclear. Here we use live-cell imaging to track the activity of a promoter under the control of the same enhancer, but inserted at different positions across a simplified regulatory landscape with minimal complexity. Combined with mathematical modeling, this reveals that RNA production from the promoter occurs in clusters of transcriptional bursts, with enhancer position controlling the frequency at which clusters appear. This results in bursts being more frequent and occurring more uniformly across cells when the enhancer is genomically close to the promoter than when it is located at a large genomic distance. Mathematical modeling further indicates that the enhancer modulates the promoter's ability to transition from its basal transcriptional state to a regime in which clusters of bursts become more frequent. Our results reveal unexplored modes of mammalian promoter operation and show that enhancer position within a cis-regulatory landscape critically controls the timing and variability of transcriptional output in single cells.
SN - 1061-4036
SN - 1546-1718
ER -