Integrated Microbial Profiling Identifies Bacterial and Fungal Communities in Pseudomyxoma Peritonei Mucin.
5 affiliations
- 1Department of Biochemistry and Molecular Biology, University of Cordoba, Cordoba, Spain.
- 2Maimonides Biomedical Research Institute of Cordoba (IMIBIC), Cordoba, Spain.
- 3Pathology Unit, Reina Sofía University Hospital, Cordoba, Spain.
- 4Unit of Surgical Oncology, Surgery Department, Reina Sofía University Hospital, Cordoba, Spain.
- 5Institute of Marine Sciences of Andalusia (ICMAN-CSIC), Cádiz, Spain.
Abstract
Pseudomyxoma peritonei (PMP) is a rare mucinous malignancy in which tumour-associated microbial communities remain poorly characterised, particularly, their fungal component and spatial organisation. We applied an integrated workflow combining 16S and 18S rRNA gene sequencing, microbial culture, histochemical staining, chromogenic RNAscope in situ hybridisation and a patient-derived xenograft model to investigate bacterial and fungal communities in control tissues and low- and high-grade PMP mucin. Bacterial and fungal DNA was detected in PMP-associated mucin, with grade-associated differences in bacterial composition and predicted functional potential. Viable bacteria were recovered from human PMP samples and xenograft-derived mucin. Fungal structures were independently supported by periodic acid-Schiff and Calcofluor White staining, while RNAscope localised bacterial 16S and fungal 18S rRNA signals within epithelial and mucin-rich tumour regions. Comparable microbial signals were observed in the xenograft model. Together, these complementary approaches identify PMP mucin as a multi-kingdom microbial niche and provide the first characterisation of its associated mycobiome. This integrated analytical framework may facilitate the investigation of microorganisms in other low-biomass, mucin-rich tumour environments and provides a basis for future mechanistic studies of microbe-host interactions in PMP.
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BibTeX
@article{pezzopane2026integrated,
title = {{Integrated Microbial Profiling Identifies Bacterial and Fungal Communities in Pseudomyxoma Peritonei Mucin.}},
author = {Rafaela Pezzopane and Marina Barbudo-Lunar and Florina I Bura and Melissa Granados-Rodríguez and Ana Martínez-López and Lidia Rodríguez-Ortiz and Blanca Rufián-Andújar and Javier López-Jiménez and Verónica Luna-Guerrero and José Alhama and Carmen Michán and Mari C Vázquez-Borrego and Álvaro Arjona-Sánchez and Antonio Romero-Ruiz},
journal = {Microbial biotechnology},
year = {2026},
month = nov,
doi = {10.1111/1751-7915.70457},
url = {https://europepmc.org/article/MED/42974864},
note = {CC BY},
}
RIS
TY - JOUR
TI - Integrated Microbial Profiling Identifies Bacterial and Fungal Communities in Pseudomyxoma Peritonei Mucin.
AU - Rafaela Pezzopane
AU - Marina Barbudo-Lunar
AU - Florina I Bura
AU - Melissa Granados-Rodríguez
AU - Ana Martínez-López
AU - Lidia Rodríguez-Ortiz
AU - Blanca Rufián-Andújar
AU - Javier López-Jiménez
AU - Verónica Luna-Guerrero
AU - José Alhama
AU - Carmen Michán
AU - Mari C Vázquez-Borrego
AU - Álvaro Arjona-Sánchez
AU - Antonio Romero-Ruiz
PY - 2026
DA - 2026/11/01
JO - Microbial biotechnology
DO - 10.1111/1751-7915.70457
UR - https://europepmc.org/article/MED/42974864
AB - Pseudomyxoma peritonei (PMP) is a rare mucinous malignancy in which tumour-associated microbial communities remain poorly characterised, particularly, their fungal component and spatial organisation. We applied an integrated workflow combining 16S and 18S rRNA gene sequencing, microbial culture, histochemical staining, chromogenic RNAscope in situ hybridisation and a patient-derived xenograft model to investigate bacterial and fungal communities in control tissues and low- and high-grade PMP mucin. Bacterial and fungal DNA was detected in PMP-associated mucin, with grade-associated differences in bacterial composition and predicted functional potential. Viable bacteria were recovered from human PMP samples and xenograft-derived mucin. Fungal structures were independently supported by periodic acid-Schiff and Calcofluor White staining, while RNAscope localised bacterial 16S and fungal 18S rRNA signals within epithelial and mucin-rich tumour regions. Comparable microbial signals were observed in the xenograft model. Together, these complementary approaches identify PMP mucin as a multi-kingdom microbial niche and provide the first characterisation of its associated mycobiome. This integrated analytical framework may facilitate the investigation of microorganisms in other low-biomass, mucin-rich tumour environments and provides a basis for future mechanistic studies of microbe-host interactions in PMP.
SN - 1751-7915
ER -