Comparative Analysis of Traditional and Modern Extraction Techniques for Phenolic Compounds From Leontopodium and Optimization of Extraction Process Using Response Surface Methodology.
- 1College of Grassland Science, Key Laboratory of Forage Cultivation, Processing and High Efficient Utilization of Ministry of Agriculture and Rural Affairs, Key Laboratory of Grassland Resources, Ministry of Education Inner Mongolia Agricultural University Hohhot China.
- 2Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences Hohhot Inner Mongolia People's Republic of China.
Abstract
Phenolic compounds from Leontopodium leontopodioides (LLB) are natural bioactive constituents with potential antioxidant activity. This study compared reflux extraction (RE), microwave-assisted extraction (MAE), and ultrasound-assisted extraction (UAE) for recovering total flavonoids (TFC) and total phenolic content (TPC) from LLB. Based on the comparative results, UAE was further optimized using response surface methodology. The effects of habitat on TFC and TPC contents and the antioxidant activity of LLB extracts were also evaluated. Under the tested operating conditions, UAE with 70% ethanol showed the best overall extraction performance and was therefore selected for optimization. The optimized UAE conditions for TFC were an extraction time of 61 min, a solid-to-liquid ratio of 1:27 (g/mL), an ultrasound power of 610 W, and an extraction temperature of 58°C, yielding 40.58 ± 0.94 mg/g DW. For TPC, the optimum conditions were 40 min, 1:24 (g/mL), 430 W, and 57°C, respectively, yielding 26.38 ± 0.57 mg/g DW. LLB collected from desert grassland exhibited the highest TFC content and the strongest DPPH-, ABTS-, and hydroxyl-radical-scavenging activities, as well as the greatest reducing power. In contrast, TPC did not differ significantly among habitats (p > 0.05). These findings provide a basis for optimizing the extraction of phenolic constituents from LLB and support its future processing and utilization as a source of bioactive ingredients.
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BibTeX
@article{yi2026comparative,
title = {{Comparative Analysis of Traditional and Modern Extraction Techniques for Phenolic Compounds From Leontopodium and Optimization of Extraction Process Using Response Surface Methodology.}},
author = {Rule Yi and Junfeng Hao and Jian Bao and Muqier Zhao and Zhijun Wang and Zhuna Bao and Gentu Ge},
journal = {Food science \& nutrition},
year = {2026},
month = oct,
doi = {10.1002/fsn3.72523},
url = {https://europepmc.org/article/MED/42953037},
note = {CC BY},
}
RIS
TY - JOUR
TI - Comparative Analysis of Traditional and Modern Extraction Techniques for Phenolic Compounds From Leontopodium and Optimization of Extraction Process Using Response Surface Methodology.
AU - Rule Yi
AU - Junfeng Hao
AU - Jian Bao
AU - Muqier Zhao
AU - Zhijun Wang
AU - Zhuna Bao
AU - Gentu Ge
PY - 2026
DA - 2026/10/27
JO - Food science & nutrition
DO - 10.1002/fsn3.72523
UR - https://europepmc.org/article/MED/42953037
AB - Phenolic compounds from Leontopodium leontopodioides (LLB) are natural bioactive constituents with potential antioxidant activity. This study compared reflux extraction (RE), microwave-assisted extraction (MAE), and ultrasound-assisted extraction (UAE) for recovering total flavonoids (TFC) and total phenolic content (TPC) from LLB. Based on the comparative results, UAE was further optimized using response surface methodology. The effects of habitat on TFC and TPC contents and the antioxidant activity of LLB extracts were also evaluated. Under the tested operating conditions, UAE with 70% ethanol showed the best overall extraction performance and was therefore selected for optimization. The optimized UAE conditions for TFC were an extraction time of 61 min, a solid-to-liquid ratio of 1:27 (g/mL), an ultrasound power of 610 W, and an extraction temperature of 58°C, yielding 40.58 ± 0.94 mg/g DW. For TPC, the optimum conditions were 40 min, 1:24 (g/mL), 430 W, and 57°C, respectively, yielding 26.38 ± 0.57 mg/g DW. LLB collected from desert grassland exhibited the highest TFC content and the strongest DPPH-, ABTS-, and hydroxyl-radical-scavenging activities, as well as the greatest reducing power. In contrast, TPC did not differ significantly among habitats (p > 0.05). These findings provide a basis for optimizing the extraction of phenolic constituents from LLB and support its future processing and utilization as a source of bioactive ingredients.
SN - 2048-7177
ER -