Molecular Detection and VP2 Characterization of Feline and Canine Parvoviruses in Domestic Cats in Central Vietnam.
4 affiliations
- 1Faculty of Veterinary Medicine, Vietnam National University of Agriculture, Hanoi 131000, Vietnam.
- 2Dak Lak Sub-Department of Livestock Production and Animal Health, Buon Ma Thuot Ward 63000, Dak Lak, Vietnam.
- 3Interdisciplinary of Genetic Engineering and Bioinformatics, Graduate School, Kasetsart University, Bangkok 10900, Thailand.
- 4Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkok 10900, Thailand.
Abstract
In this study, 269 fecal specimens were sampled from domestic cats displaying symptomatic and asymptomatic individuals in Daklak and Gialai provinces in Central Vietnam during July 2023 to July 2025. The polymerase chain reaction method was applied to determine the feline parvovirus DNA in the obtained samples. Positive samples were randomly chosen for full-length VP2 gene sequencing using the Sanger method. The feline parvovirus DNA was detected in 25 (9.29%) of 269 samples. The viral DNA-positive rate was 17.65% (15/85) from cats aged below 6 months, significantly higher than that from 6-12- and >12-month-old cats. Seven complete VP2 sequences were successfully obtained from the identified strains, exhibiting a high degree of nucleotide identity (98.23-100%). Comparison to that of other sequences retrieved from GenBank indicated that seven current Vietnamese sequences displayed the highest nucleotide identity was from 99.08 to 99.94 with that of viral strains from China, Italy, and Spain. Phylogenetic analysis constructed using the complete VP2 gene sequences indicated that six Vietnamese sequences obtained belonged to feline panleukopenia virus (FPV) lineage and divided into several clusters, which were closely related to viruses from China, Italy, and Spain. The remaining one sequence was defined as canine parvovirus (CPV) which was genetically related to previous Vietnamese CPV-2c and Chinese strains. Twenty-six negative selection sites were detected in the VP2 protein of the current Vietnamese FPV/CPV strains in this study.
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BibTeX
@article{tran2026molecular,
title = {{Molecular Detection and VP2 Characterization of Feline and Canine Parvoviruses in Domestic Cats in Central Vietnam.}},
author = {Giang Huong Thi Tran and Ngan Hong Thi Le and Amonpun Rattanasrisomporn and Jatuporn Rattanasrisomporn},
journal = {International journal of molecular sciences},
year = {2026},
month = sep,
doi = {10.3390/ijms27198541},
url = {https://europepmc.org/article/MED/42883788},
note = {CC BY},
}
RIS
TY - JOUR
TI - Molecular Detection and VP2 Characterization of Feline and Canine Parvoviruses in Domestic Cats in Central Vietnam.
AU - Giang Huong Thi Tran
AU - Ngan Hong Thi Le
AU - Amonpun Rattanasrisomporn
AU - Jatuporn Rattanasrisomporn
PY - 2026
DA - 2026/09/24
JO - International journal of molecular sciences
DO - 10.3390/ijms27198541
UR - https://europepmc.org/article/MED/42883788
AB - In this study, 269 fecal specimens were sampled from domestic cats displaying symptomatic and asymptomatic individuals in Daklak and Gialai provinces in Central Vietnam during July 2023 to July 2025. The polymerase chain reaction method was applied to determine the feline parvovirus DNA in the obtained samples. Positive samples were randomly chosen for full-length VP2 gene sequencing using the Sanger method. The feline parvovirus DNA was detected in 25 (9.29%) of 269 samples. The viral DNA-positive rate was 17.65% (15/85) from cats aged below 6 months, significantly higher than that from 6-12- and >12-month-old cats. Seven complete VP2 sequences were successfully obtained from the identified strains, exhibiting a high degree of nucleotide identity (98.23-100%). Comparison to that of other sequences retrieved from GenBank indicated that seven current Vietnamese sequences displayed the highest nucleotide identity was from 99.08 to 99.94 with that of viral strains from China, Italy, and Spain. Phylogenetic analysis constructed using the complete VP2 gene sequences indicated that six Vietnamese sequences obtained belonged to feline panleukopenia virus (FPV) lineage and divided into several clusters, which were closely related to viruses from China, Italy, and Spain. The remaining one sequence was defined as canine parvovirus (CPV) which was genetically related to previous Vietnamese CPV-2c and Chinese strains. Twenty-six negative selection sites were detected in the VP2 protein of the current Vietnamese FPV/CPV strains in this study.
SN - 1422-0067
ER -