Quantitative Characterization of RNA-Splinted Ligation as a Multipurpose Post-Synthetic Strategy for High-Density DNA Microarrays Synthesized by Nucleic Acid Photolithography.
5 affiliations
- 1Technical University of Munich , Garching85748, Germany.
- 2TUM School of Natural Sciences, Garching85748, Germany.
- 3Leibniz Institute for Food Systems Biology at the Technical University of Munich , Freising85354, Germany.
- 4Chair of Food Chemistry and Molecular Sensory Science, Technical University of Munich, Freising85354, Germany.
- 5Institute of Inorganic Chemistry, Faculty of Chemistry, University of Vienna, Vienna1090, Austria.
Abstract
Complex arrays of surface-immobilized DNA oligonucleotides are widely used across diverse fields of bioanalytics, particularly for their applications in massively parallel genomic assays ranging from spatial transcriptomics to protein-DNA recognition studies. DNA microarrays produced by photolithography enable the introduction of versatile chemistries and complexities to surfaces with complete spatial control, but are partially constrained by synthetic error rates for longer probe architectures and by incompatibility with photosensitive chemical building blocks commonly used in phosphoramidite chemistry. Here, we present RNA-splinted ligation as a multipurpose post-synthetic microarray fabrication strategy for extending the complexity of DNA on microarray surfaces. We systematically characterize the parameters governing on-array DNA ligation and demonstrate its applications in labeling, reverse transcription, and the generation of highly complex patterns by introducing an otherwise incompatible photochemistry.
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BibTeX
@article{das2026quantitative,
title = {{Quantitative Characterization of RNA-Splinted Ligation as a Multipurpose Post-Synthetic Strategy for High-Density DNA Microarrays Synthesized by Nucleic Acid Photolithography.}},
author = {Arya Das and Mark M Somoza},
journal = {Analytical chemistry},
year = {2026},
month = sep,
doi = {10.1021/acs.analchem.6c03811},
url = {https://europepmc.org/article/MED/42916387},
note = {CC BY},
}
RIS
TY - JOUR
TI - Quantitative Characterization of RNA-Splinted Ligation as a Multipurpose Post-Synthetic Strategy for High-Density DNA Microarrays Synthesized by Nucleic Acid Photolithography.
AU - Arya Das
AU - Mark M Somoza
PY - 2026
DA - 2026/09/01
JO - Analytical chemistry
DO - 10.1021/acs.analchem.6c03811
UR - https://europepmc.org/article/MED/42916387
AB - Complex arrays of surface-immobilized DNA oligonucleotides are widely used across diverse fields of bioanalytics, particularly for their applications in massively parallel genomic assays ranging from spatial transcriptomics to protein-DNA recognition studies. DNA microarrays produced by photolithography enable the introduction of versatile chemistries and complexities to surfaces with complete spatial control, but are partially constrained by synthetic error rates for longer probe architectures and by incompatibility with photosensitive chemical building blocks commonly used in phosphoramidite chemistry. Here, we present RNA-splinted ligation as a multipurpose post-synthetic microarray fabrication strategy for extending the complexity of DNA on microarray surfaces. We systematically characterize the parameters governing on-array DNA ligation and demonstrate its applications in labeling, reverse transcription, and the generation of highly complex patterns by introducing an otherwise incompatible photochemistry.
SN - 0003-2700
SN - 1520-6882
ER -