Effects of Corydalis saxicola Bunting total alkaloids on lipopolysaccharide-induced inflammatory responses in human oral epithelial cells
- 1Department of Periodontics and Oral Medicine, College of Stomatology, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
- 2Pharmaceutical College, Guangxi Medical University, Nanning, Guangxi 530021, P.R. China
Abstract
Oral mucositis is a common inflammatory condition affecting the oral mucosa, for which effective therapeutic options remain limited. Corydalis saxicola Bunting total alkaloids (CSBTA) have been reported to exhibit anti-inflammatory activity in several experimental models; however, their effects on inflammatory responses in oral epithelial cells have not been fully elucidated. The present study evaluated the effects of CSBTA on lipopolysaccharide (LPS)-induced inflammation in human oral epithelial cells (HOECs) and explored potential molecular mechanisms. Network pharmacology analysis and molecular docking were employed as supplementary approaches to predict potential targets associated with CSBTA and oral mucositis. An in vitro inflammatory model was established by stimulating HOECs with LPS. The mRNA expression levels of IL-1β, IL-6, IL-8, TNF-α and activator protein 1 (AP-1) were determined using reverse transcription-quantitative PCR. The concentrations of pro-inflammatory cytokines in cell culture supernatants were measured by enzyme-linked immunosorbent assay and the protein expression levels of c-Jun and phosphorylated c-Jun were assessed by western blotting. Network pharmacology analysis suggested that JUN may be associated with the effects of CSBTA and molecular docking indicated favorable binding between JUN and several major alkaloid components of CSBTA. In vitro experiments demonstrated that CSBTA markedly reduced LPS-induced upregulation of IL-1β, IL-6, IL-8 and TNF-α in HOECs. In addition, CSBTA decreased the mRNA and protein expression levels of AP-1-related markers, with effects comparable to those observed following treatment with the AP-1 inhibitor SR11302. In conclusion, CSBTA attenuated LPS-induced inflammatory responses in human oral epithelial cells. These effects may be associated with modulation of AP-1-related signaling, suggesting that CSBTA may warrant further investigation for oral mucosal inflammatory conditions.
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BibTeX
@article{qin2026effects,
title = {{Effects of Corydalis saxicola Bunting total alkaloids on lipopolysaccharide-induced inflammatory responses in human oral epithelial cells}},
author = {Yashi Qin and Yiyuan Lai and Jiaxuan Wu and Qiaozhi Jiang and Zhiheng Su and Zhenmin Liu and Renchuan Tao and Xiangzhi Yong},
journal = {Biomedical reports},
year = {2026},
month = oct,
doi = {10.3892/br.2026.2219},
url = {https://europepmc.org/article/PMC/PMC13729704},
note = {CC BY-NC-ND},
}
RIS
TY - JOUR
TI - Effects of Corydalis saxicola Bunting total alkaloids on lipopolysaccharide-induced inflammatory responses in human oral epithelial cells
AU - Yashi Qin
AU - Yiyuan Lai
AU - Jiaxuan Wu
AU - Qiaozhi Jiang
AU - Zhiheng Su
AU - Zhenmin Liu
AU - Renchuan Tao
AU - Xiangzhi Yong
PY - 2026
DA - 2026/10/23
JO - Biomedical reports
DO - 10.3892/br.2026.2219
UR - https://europepmc.org/article/PMC/PMC13729704
AB - Oral mucositis is a common inflammatory condition affecting the oral mucosa, for which effective therapeutic options remain limited. Corydalis saxicola Bunting total alkaloids (CSBTA) have been reported to exhibit anti-inflammatory activity in several experimental models; however, their effects on inflammatory responses in oral epithelial cells have not been fully elucidated. The present study evaluated the effects of CSBTA on lipopolysaccharide (LPS)-induced inflammation in human oral epithelial cells (HOECs) and explored potential molecular mechanisms. Network pharmacology analysis and molecular docking were employed as supplementary approaches to predict potential targets associated with CSBTA and oral mucositis. An in vitro inflammatory model was established by stimulating HOECs with LPS. The mRNA expression levels of IL-1β, IL-6, IL-8, TNF-α and activator protein 1 (AP-1) were determined using reverse transcription-quantitative PCR. The concentrations of pro-inflammatory cytokines in cell culture supernatants were measured by enzyme-linked immunosorbent assay and the protein expression levels of c-Jun and phosphorylated c-Jun were assessed by western blotting. Network pharmacology analysis suggested that JUN may be associated with the effects of CSBTA and molecular docking indicated favorable binding between JUN and several major alkaloid components of CSBTA. In vitro experiments demonstrated that CSBTA markedly reduced LPS-induced upregulation of IL-1β, IL-6, IL-8 and TNF-α in HOECs. In addition, CSBTA decreased the mRNA and protein expression levels of AP-1-related markers, with effects comparable to those observed following treatment with the AP-1 inhibitor SR11302. In conclusion, CSBTA attenuated LPS-induced inflammatory responses in human oral epithelial cells. These effects may be associated with modulation of AP-1-related signaling, suggesting that CSBTA may warrant further investigation for oral mucosal inflammatory conditions.
SN - 2049-9434
SN - 2049-9442
ER -