Dietary dimethylglycine sodium salt attenuates lipopolysaccharide-associated hepatic alterations and modulates redox responses in broiler chickens
- 1College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou, 310023, China
- 2School of Biological and Chemical Engineering, Zhejiang University of Science and Technology, Hangzhou, 310023, China
Abstract
Repeated lipopolysaccharide (LPS) exposure compromises broiler growth and hepatic homeostasis. This study investigated the effects of dietary dimethylglycine sodium salt (DMG-Na) on hepatic responses to repeated LPS challenge and breast-muscle quality. A total of 320 one-day-old male Arbor Acres broilers were assigned to a 2 × 2 factorial design with 0 or 0.4 g DMG-Na/kg basal diet and saline or LPS (10 cages of 8 birds per treatment). Lipopolysaccharide (1 mg/kg body weight) was administered intraperitoneally on d 17, 19, and 21. DMG-Na × LPS interactions were detected for postchallenge average daily gain (P = 0.038), feed conversion ratio (P = 0.031), hepatic histopathological score (P = 0.013), and swollen mitochondrial profiles (P = 0.002). Relative to LPS alone, DMG-Na supplementation reduced histopathological score, mitochondrial swelling, and fraction-associated protein carbonyl content and was accompanied by lower hepatic SIRT1 protein abundance and phosphorylated NF-κB p65 abundance in LPSD birds (P < 0.05). Factorial transcriptomics and metabolomics identified 138 interaction-associated genes and 126 metabolite features, respectively, involving inflammatory and redox-related processes. Hepatic mtDNA abundance increased, whereas ATP and respiratory-chain complex activities remained unchanged. Breast-muscle drip loss showed an interaction (P = 0.041), but supplementation did not significantly alter drip loss within LPS-challenged birds. The treatment-only regression model outperformed the hepatic-variable random forest in predicting drip loss. Dietary DMG-Na improved postchallenge feed efficiency and attenuated hepatic inflammatory, redox, and structural alterations in LPS-challenged broilers.
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BibTeX
@article{jiang2026dietary,
title = {{Dietary dimethylglycine sodium salt attenuates lipopolysaccharide-associated hepatic alterations and modulates redox responses in broiler chickens}},
author = {Luyi Jiang and Yaqi Rao and Kaiwen Bai},
journal = {Poultry science},
year = {2026},
month = oct,
doi = {10.1016/j.psj.2026.107994},
url = {https://europepmc.org/article/PMC/PMC13718808},
note = {CC BY},
}
RIS
TY - JOUR
TI - Dietary dimethylglycine sodium salt attenuates lipopolysaccharide-associated hepatic alterations and modulates redox responses in broiler chickens
AU - Luyi Jiang
AU - Yaqi Rao
AU - Kaiwen Bai
PY - 2026
DA - 2026/10/20
JO - Poultry science
DO - 10.1016/j.psj.2026.107994
UR - https://europepmc.org/article/PMC/PMC13718808
AB - Repeated lipopolysaccharide (LPS) exposure compromises broiler growth and hepatic homeostasis. This study investigated the effects of dietary dimethylglycine sodium salt (DMG-Na) on hepatic responses to repeated LPS challenge and breast-muscle quality. A total of 320 one-day-old male Arbor Acres broilers were assigned to a 2 × 2 factorial design with 0 or 0.4 g DMG-Na/kg basal diet and saline or LPS (10 cages of 8 birds per treatment). Lipopolysaccharide (1 mg/kg body weight) was administered intraperitoneally on d 17, 19, and 21. DMG-Na × LPS interactions were detected for postchallenge average daily gain (P = 0.038), feed conversion ratio (P = 0.031), hepatic histopathological score (P = 0.013), and swollen mitochondrial profiles (P = 0.002). Relative to LPS alone, DMG-Na supplementation reduced histopathological score, mitochondrial swelling, and fraction-associated protein carbonyl content and was accompanied by lower hepatic SIRT1 protein abundance and phosphorylated NF-κB p65 abundance in LPSD birds (P < 0.05). Factorial transcriptomics and metabolomics identified 138 interaction-associated genes and 126 metabolite features, respectively, involving inflammatory and redox-related processes. Hepatic mtDNA abundance increased, whereas ATP and respiratory-chain complex activities remained unchanged. Breast-muscle drip loss showed an interaction (P = 0.041), but supplementation did not significantly alter drip loss within LPS-challenged birds. The treatment-only regression model outperformed the hepatic-variable random forest in predicting drip loss. Dietary DMG-Na improved postchallenge feed efficiency and attenuated hepatic inflammatory, redox, and structural alterations in LPS-challenged broilers.
SN - 0032-5791
SN - 1525-3171
ER -