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Peer-reviewedCC BYScientific Reports

Non-GMO surface display of VP28 on Pediococcus pentosaceus for oral protection against white spot syndrome virus in shrimp

  • Pinpunya Riangrungroj1
  • Suparat Taengchaiyaphum2
  • Somphop Boonpayung1
  • Siwaporn Longyant3
  • Dujduan Waraho-Zhmayev4
  • Kallaya Sritunyalucksana2
  • Ubolsree Leartsakulpanich1
4 affiliations
  1. 1National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)
  2. 2Aquatic Animal Health Research Team, Integrative Aquaculture Biotechnology Research Group, National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA)
  3. 3Department of Biology, Faculty of Science, Srinakharinwirot University
  4. 4Biological Engineering Program, Faculty of Engineering, King Mongkut’s University of Technology Thonburi
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Abstract

VP28, a major envelope protein of White Spot Syndrome Virus (WSSV), plays a critical role in viral entry and infection, making it an important target for developing novel control measures against this globally devastating shrimp pathogen. This study developed a non-GMO oral shrimp vaccine by externally immobilizing the WSSV VP28 protein on the surface of food-grade Pediococcus pentosaceus BT520 (Pp). VP28 was fused to the C-terminal domain of Lactococcus lactis N-acetylmuramidase AcmA (cA), a well-characterized cell wall binding module, and the resulting fusion protein was expressed, purified, and immobilized onto Pp cells. Surface display was confirmed by Western blot, flow cytometry and immunofluorescence microscopy. Its specific interaction with the shrimp Rab7 protein was also confirmed, and approximately 105 cA domains were estimated per bacterial cell. The VP28-displaying cells (Pp-VP28) remained stable in 25 ppt artificial seawater for up to 24 h. Oral administration of Pp-VP28 at 108 CFU g−1 feed to Penaeus vannamei (Pacific whiteleg shrimp) for 7 days yielded a relative percent survival (RPS) of 76.25 ± 3.75%, demonstrating marked protection against WSSV. These results highlight the potential of this non-GMO display platform for practical, scalable oral vaccination in sustainable shrimp aquaculture.

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Cite

BibTeXRIS
BibTeX
@article{riangrungroj2026nongmo,
  title = {{Non-GMO surface display of VP28 on Pediococcus pentosaceus for oral protection against white spot syndrome virus in shrimp}},
  author = {Pinpunya Riangrungroj and Suparat Taengchaiyaphum and Somphop Boonpayung and Siwaporn Longyant and Dujduan Waraho-Zhmayev and Kallaya Sritunyalucksana and Ubolsree Leartsakulpanich},
  journal = {Scientific Reports},
  year = {2026},
  month = jul,
  doi = {10.1038/s41598-026-63552-6},
  url = {https://doi.org/10.1038/s41598-026-63552-6},
  note = {CC BY},
}
RIS
TY  - JOUR
TI  - Non-GMO surface display of VP28 on Pediococcus pentosaceus for oral protection against white spot syndrome virus in shrimp
AU  - Pinpunya Riangrungroj
AU  - Suparat Taengchaiyaphum
AU  - Somphop Boonpayung
AU  - Siwaporn Longyant
AU  - Dujduan Waraho-Zhmayev
AU  - Kallaya Sritunyalucksana
AU  - Ubolsree Leartsakulpanich
PY  - 2026
DA  - 2026/07/01
JO  - Scientific Reports
DO  - 10.1038/s41598-026-63552-6
UR  - https://doi.org/10.1038/s41598-026-63552-6
AB  - VP28, a major envelope protein of White Spot Syndrome Virus (WSSV), plays a critical role in viral entry and infection, making it an important target for developing novel control measures against this globally devastating shrimp pathogen. This study developed a non-GMO oral shrimp vaccine by externally immobilizing the WSSV VP28 protein on the surface of food-grade Pediococcus pentosaceus BT520 (Pp). VP28 was fused to the C-terminal domain of Lactococcus lactis N-acetylmuramidase AcmA (cA), a well-characterized cell wall binding module, and the resulting fusion protein was expressed, purified, and immobilized onto Pp cells. Surface display was confirmed by Western blot, flow cytometry and immunofluorescence microscopy. Its specific interaction with the shrimp Rab7 protein was also confirmed, and approximately 105 cA domains were estimated per bacterial cell. The VP28-displaying cells (Pp-VP28) remained stable in 25 ppt artificial seawater for up to 24 h. Oral administration of Pp-VP28 at 108 CFU g−1 feed to Penaeus vannamei (Pacific whiteleg shrimp) for 7 days yielded a relative percent survival (RPS) of 76.25 ± 3.75%, demonstrating marked protection against WSSV. These results highlight the potential of this non-GMO display platform for practical, scalable oral vaccination in sustainable shrimp aquaculture.
SN  - 2045-2322
ER  -