Peer-reviewedCC BYScientific Reports
Development of a novel phage-displayed fusion protein of newcastle disease virus as a highly specific antigen for serological assays
8 affiliations
- 1School of Animal and Veterinary Sciences, Adelaide University
- 2Institute for Applied Ecology, University of Canberra
- 3State Key Laboratory of Genetics and Development of Complex Phenotypes, School of Life Sciences, Fudan University
- 4Research Organization for Health, National Research and Innovation Agency (BRIN)
- 5Research Center for Veterinary Science, Research Organization for Health, National Research and Innovation Agency (BRIN)
- 6Department of Pathobiology, School of Veterinary Medicine, Shiraz University
- 7UniSA Clinical & Health Sciences, University of South Australia
- 8Universitas Nusa Cendana, Fakultas Kedokteran dan Kedokteran Hewan(FKKH)
Abstract
Newcastle disease virus is one of the most highly contagious infections impacting poultry industries worldwide. This study aimed to develop a bacteriophage display system as a cost-effective and immunogenic method for expressing the immunodominant region of the Newcastle Disease Virus Fusion (F) protein for use in serological assays. Both in-silico and in-vitro analyses indicated that the selected antigen is stable, accurate, and reliable for further in-vivo studies. The displayed antigen successfully detected NDV-GVII-specific antibodies in vaccinated chickens. Additionally, a significant correlation was observed between ELISA and HI test results on chicken serum samples. This approach addresses safety concerns associated with wild-type virulent NDVs and offers improved diagnostic accuracy and scalability, aligning with industry needs for improved Newcastle disease detection, control, and management on poultry farms.
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BibTeX
@article{najafi2026development,
title = {{Development of a novel phage-displayed fusion protein of newcastle disease virus as a highly specific antigen for serological assays}},
author = {Mehrnaz Najafi and Hanh Thi Hong Nguyen and Bahareh Pakbaten and Aliakbar Khabiri and Abdollah Derakhshandeh and Chen Ling and Ni Luh Puti Indi Dharmayanti and Harimurti Nuradji and Faidah Rahmawati and Rahmat Setya Adji and Nuha Fairunisya and Fitirine Ekawasti and Prima Mei Widiy and Tati Ariyanti and Ngoc X. B. Nguyen and Kandarp Patel and Maryam Sadat Moezzi and Mohammad Indro Cahyono and Sammi Yuen Tung Ngai and Putri Pandarangga and Farhid Hemmatzadeh},
journal = {Scientific Reports},
year = {2026},
month = jul,
doi = {10.1038/s41598-026-60560-4},
url = {https://doi.org/10.1038/s41598-026-60560-4},
note = {CC BY},
}
RIS
TY - JOUR
TI - Development of a novel phage-displayed fusion protein of newcastle disease virus as a highly specific antigen for serological assays
AU - Mehrnaz Najafi
AU - Hanh Thi Hong Nguyen
AU - Bahareh Pakbaten
AU - Aliakbar Khabiri
AU - Abdollah Derakhshandeh
AU - Chen Ling
AU - Ni Luh Puti Indi Dharmayanti
AU - Harimurti Nuradji
AU - Faidah Rahmawati
AU - Rahmat Setya Adji
AU - Nuha Fairunisya
AU - Fitirine Ekawasti
AU - Prima Mei Widiy
AU - Tati Ariyanti
AU - Ngoc X. B. Nguyen
AU - Kandarp Patel
AU - Maryam Sadat Moezzi
AU - Mohammad Indro Cahyono
AU - Sammi Yuen Tung Ngai
AU - Putri Pandarangga
AU - Farhid Hemmatzadeh
PY - 2026
DA - 2026/07/01
JO - Scientific Reports
DO - 10.1038/s41598-026-60560-4
UR - https://doi.org/10.1038/s41598-026-60560-4
AB - Newcastle disease virus is one of the most highly contagious infections impacting poultry industries worldwide. This study aimed to develop a bacteriophage display system as a cost-effective and immunogenic method for expressing the immunodominant region of the Newcastle Disease Virus Fusion (F) protein for use in serological assays. Both in-silico and in-vitro analyses indicated that the selected antigen is stable, accurate, and reliable for further in-vivo studies. The displayed antigen successfully detected NDV-GVII-specific antibodies in vaccinated chickens. Additionally, a significant correlation was observed between ELISA and HI test results on chicken serum samples. This approach addresses safety concerns associated with wild-type virulent NDVs and offers improved diagnostic accuracy and scalability, aligning with industry needs for improved Newcastle disease detection, control, and management on poultry farms.
SN - 2045-2322
ER -