Gold-Enhanced Multielectrode Electrochemical Immunosensor for Rapid Electrochemical Detection of Dengue NS1
5 affiliations
- 1State University of Campinas , , , ,
- 2Center for Innovation in New Energies , , ,
- 3Federal University of São Carlos , , , ,
- 4Faculdade São Leopoldo Mandic , , ,
- 5ADB Pesquisa e Desenvolvimento Ltda , , ,
Abstract
Dengue is one of the most widespread viral diseases in the world, transmitted mainly by Aedes aegypti and Aedes albopictus. In tropical and resource-limited regions, rapid and accurate diagnostic tools are of utmost importance, especially when aiming for effective control, but current detection methods face limitations in sensitivity, specificity, or accessibility. In this work, a multielectrode disposable electrochemical immunosensor was developed for the detection of the nonstructural protein 1 (NS1), an early biomarker of dengue infection. This device presents four fully individual and functional working electrodes modified with gold nanoparticles. This configuration enables replicate measurements and improved reproducibility within a single platform while using minimal sample volume. The electrodeposition of Au nanoparticles was optimized through Design of Experiments approaches, allowing the system to suitably determine the target protein with a specific biorecognition architecture based on antibody–antigen recognition. This biosensor was characterized by morphological and electrochemical techniques, achieving a linear response between 1.0 and 200 ng mL–1 and a limit of detection of 0.34 ng mL–1. Afterward, human serum samples spiked with NS1 showed recovery rates between 96.6% and 115%. All results indicate that the proposed multielectrode immunosensor is a promising, label-free, and portable diagnostic alternative for NS1 determination while maintaining a relatively low cost and simple preparation.
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BibTeX
@article{orzari2026goldenhanced,
title = {{Gold-Enhanced Multielectrode Electrochemical Immunosensor for Rapid Electrochemical Detection of Dengue NS1}},
author = {Luiz Otávio Orzari and Bruna Silva Faria and Jéssica Rocha Camargo and Lucas Aguilera Mariano and Déborah Christine Azzi and Alexandre Gatti and Geisiany Maria de Queiroz and Bruno Campos Janegitz},
journal = {ACS Omega},
year = {2026},
month = oct,
doi = {10.1021/acsomega.6c04898},
url = {https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c04898/5454019/Gold-Enhanced-Multielectrode-Electrochemical},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Gold-Enhanced Multielectrode Electrochemical Immunosensor for Rapid Electrochemical Detection of Dengue NS1
AU - Luiz Otávio Orzari
AU - Bruna Silva Faria
AU - Jéssica Rocha Camargo
AU - Lucas Aguilera Mariano
AU - Déborah Christine Azzi
AU - Alexandre Gatti
AU - Geisiany Maria de Queiroz
AU - Bruno Campos Janegitz
PY - 2026
DA - 2026/10/19
JO - ACS Omega
DO - 10.1021/acsomega.6c04898
UR - https://pubs.acs.org/acsodf/article/doi/10.1021/acsomega.6c04898/5454019/Gold-Enhanced-Multielectrode-Electrochemical
AB - Dengue is one of the most widespread viral diseases in the world, transmitted mainly by Aedes aegypti and Aedes albopictus. In tropical and resource-limited regions, rapid and accurate diagnostic tools are of utmost importance, especially when aiming for effective control, but current detection methods face limitations in sensitivity, specificity, or accessibility. In this work, a multielectrode disposable electrochemical immunosensor was developed for the detection of the nonstructural protein 1 (NS1), an early biomarker of dengue infection. This device presents four fully individual and functional working electrodes modified with gold nanoparticles. This configuration enables replicate measurements and improved reproducibility within a single platform while using minimal sample volume. The electrodeposition of Au nanoparticles was optimized through Design of Experiments approaches, allowing the system to suitably determine the target protein with a specific biorecognition architecture based on antibody–antigen recognition. This biosensor was characterized by morphological and electrochemical techniques, achieving a linear response between 1.0 and 200 ng mL–1 and a limit of detection of 0.34 ng mL–1. Afterward, human serum samples spiked with NS1 showed recovery rates between 96.6% and 115%. All results indicate that the proposed multielectrode immunosensor is a promising, label-free, and portable diagnostic alternative for NS1 determination while maintaining a relatively low cost and simple preparation.
SN - 2470-1343
ER -