Conserved and Tissue‐Specific RNA and Protein Cargos of Small Extracellular Vesicles From 120 Tissue Sites Across 34 Organs in a Single Pig
- 1National Key Laboratory for Pig Genetic Improvement and Germplasm Innovation Jiangxi Agricultural University Nanchang Jiangxi China
Abstract
Small extracellular vesicles (sEVs) carry diverse cargo essential for intercellular communication, but their organism‐wide characterization across all tissues is still limited. Here, we present a multi‐omics sEV atlas covering the whole‐body of 120 tissue sites, four body fluids and nine gastrointestinal contents from the same pig. We identified 7995 proteins, 337 miRNAs as well as 105,409 transcripts (93,409 full‐length) in sEVs by mass spectrometry, next‐ and third‐generation RNA sequencing. Among these, a conserved cargo set of 252 proteins, 18 miRNAs and 1227 transcripts was defined, including RNA‐binding proteins (ANXA2, ALIX and MVP), mitochondrial proteins (ATP5F1A, COX6C and VDACs), the let‐7 miRNA family and ferritin genes (FTH1/FTL). Notably, sEV biogenesis‐associated ACTG1, CFL1 and ANXA2 were detected in all samples, suggesting their potential as novel sEV protein markers. In contrast, 2638 proteins, 152 miRNAs and 1000 transcripts showed organ‐specific expression patterns, which are likely to underpin organ‐specific biological processes. Moreover, by using this multi‐omic dataset and additional RIP assays, we found that HNRNPU participates in the sorting of hypoxia‐responsive EPAS1 into sEVs. This comprehensive sEV atlas establishes a foundation for future studies in sEV cell biology.
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BibTeX
@article{yu2026conserved,
title = {{Conserved and Tissue‐Specific RNA and Protein Cargos of Small Extracellular Vesicles From 120 Tissue Sites Across 34 Organs in a Single Pig}},
author = {Naixiang Yu and Jinxiu Liu and Chenliang Lai and Siyi Liu and Jiacheng Wei and Jiahong Zhong and Sanya Xiong and Qingjie Zeng and Junwu Ma and Zhou Zhang and Zhiyan Zhang},
journal = {Journal of Extracellular Vesicles},
year = {2026},
month = oct,
doi = {10.1002/jev2.70374},
url = {https://doi.org/10.1002/jev2.70374},
note = {CC BY},
}
RIS
TY - JOUR
TI - Conserved and Tissue‐Specific RNA and Protein Cargos of Small Extracellular Vesicles From 120 Tissue Sites Across 34 Organs in a Single Pig
AU - Naixiang Yu
AU - Jinxiu Liu
AU - Chenliang Lai
AU - Siyi Liu
AU - Jiacheng Wei
AU - Jiahong Zhong
AU - Sanya Xiong
AU - Qingjie Zeng
AU - Junwu Ma
AU - Zhou Zhang
AU - Zhiyan Zhang
PY - 2026
DA - 2026/10/26
JO - Journal of Extracellular Vesicles
DO - 10.1002/jev2.70374
UR - https://doi.org/10.1002/jev2.70374
AB - Small extracellular vesicles (sEVs) carry diverse cargo essential for intercellular communication, but their organism‐wide characterization across all tissues is still limited. Here, we present a multi‐omics sEV atlas covering the whole‐body of 120 tissue sites, four body fluids and nine gastrointestinal contents from the same pig. We identified 7995 proteins, 337 miRNAs as well as 105,409 transcripts (93,409 full‐length) in sEVs by mass spectrometry, next‐ and third‐generation RNA sequencing. Among these, a conserved cargo set of 252 proteins, 18 miRNAs and 1227 transcripts was defined, including RNA‐binding proteins (ANXA2, ALIX and MVP), mitochondrial proteins (ATP5F1A, COX6C and VDACs), the let‐7 miRNA family and ferritin genes (FTH1/FTL). Notably, sEV biogenesis‐associated ACTG1, CFL1 and ANXA2 were detected in all samples, suggesting their potential as novel sEV protein markers. In contrast, 2638 proteins, 152 miRNAs and 1000 transcripts showed organ‐specific expression patterns, which are likely to underpin organ‐specific biological processes. Moreover, by using this multi‐omic dataset and additional RIP assays, we found that HNRNPU participates in the sorting of hypoxia‐responsive EPAS1 into sEVs. This comprehensive sEV atlas establishes a foundation for future studies in sEV cell biology.
SN - 2001-3078
ER -