Peer-reviewedCC BYmicroPublication Biology
Caenorhabditis Intervention Testing Program: all-trans retinoic acid lifespan extension in Caenorhabditis elegans requires 5-Fluoro-2'-deoxyuridine (FUdR)
- 1Institute of Ecology and Evolution, University of Oregon, Eugene, Oregon, USA
- 2The Buck Institute for Research on Aging, Novato, California, USA
- 3Department of Molecular Biology and Biochemistry, Rutgers University, Piscataway, New Jersey, USA
Abstract
The Caenorhabditis Intervention Testing Program recently characterized the longevity-promoting effects of all-trans-retinoic acid (atRA) in C. elegans. While we observed that atRA extended lifespan across multiple genetic backgrounds, environmental factors may influence its efficacy. One such factor is 5-Fluoro-2'-deoxyuridine (FUdR), used to prevent progeny contamination in lifespan assays. Here, we show that FUdR enhances atRA's lifespan effects, but that this potentiation is not simply due to progeny elimination, as sterilization through auxin-mediated disruption of spermatogenesis does not potentiate atRA in lifespan extension. We conclude that atRA longevity is dependent on additional effects of FUdR that remain uncharacterized.
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BibTeX
@article{banse2026caenorhabditis,
title = {{Caenorhabditis Intervention Testing Program: all-trans retinoic acid lifespan extension in Caenorhabditis elegans requires 5-Fluoro-2'-deoxyuridine (FUdR)}},
author = {Stephen A. Banse and Anna L. Coleman-Hulbert and Christine A. Sedore and Erik Johnson and Gordon J. Lithgow and Monica Driscoll and Patrick C. Phillips},
journal = {microPublication Biology},
year = {2026},
month = aug,
doi = {10.17912/micropub.biology.002343},
url = {https://doi.org/10.17912/micropub.biology.002343},
note = {CC BY},
}
RIS
TY - JOUR
TI - Caenorhabditis Intervention Testing Program: all-trans retinoic acid lifespan extension in Caenorhabditis elegans requires 5-Fluoro-2'-deoxyuridine (FUdR)
AU - Stephen A. Banse
AU - Anna L. Coleman-Hulbert
AU - Christine A. Sedore
AU - Erik Johnson
AU - Gordon J. Lithgow
AU - Monica Driscoll
AU - Patrick C. Phillips
PY - 2026
DA - 2026/08/01
JO - microPublication Biology
DO - 10.17912/micropub.biology.002343
UR - https://doi.org/10.17912/micropub.biology.002343
AB - The Caenorhabditis Intervention Testing Program recently characterized the longevity-promoting effects of all-trans-retinoic acid (atRA) in C. elegans. While we observed that atRA extended lifespan across multiple genetic backgrounds, environmental factors may influence its efficacy. One such factor is 5-Fluoro-2'-deoxyuridine (FUdR), used to prevent progeny contamination in lifespan assays. Here, we show that FUdR enhances atRA's lifespan effects, but that this potentiation is not simply due to progeny elimination, as sterilization through auxin-mediated disruption of spermatogenesis does not potentiate atRA in lifespan extension. We conclude that atRA longevity is dependent on additional effects of FUdR that remain uncharacterized.
SN - 2578-9430
ER -