Silver Nanoparticles Suppress Apple Fire Blight: Antibacterial Activity, Reduced Carbohydrate-Containing EPS Content, and Altered Host Antioxidant Responses.
6 affiliations
- 1Department of Chemistry, M. V. Lomonosov Moscow State University, Moscow 119991, Russia.
- 2NTI Center "Digital Materials Science: New Materials and Substances", Bauman Moscow State Technical University, Moscow 105005, Russia.
- 3Department of Testing Growth Regulators and Agrochemicals, Pryanishnikov All-Russian Scientific Research Institute of Agrochemistry, Moscow 127550, Russia.
- 4Institute of Ecology, Biotechnology and Nature Management, Kazan Federal University, Kazan 420008, Russia.
- 5Software Engineering, Institute of Computer Technologies and AI, Kyrgyz National University named after J. Balasagyn, Bishkek 720000, Kyrgyzstan.
- 6Impactive Technologies Holding Limited, Abu Dhabi P.O. Box 95044, United Arab Emirates.
Abstract
The present study evaluated the phytoprotective activity of silver nanoparticles stabilized with sodium amphopolycarboxyglycinate (AgNPs-APCG) against apple fire blight caused by Erwinia amylovora. A highly colloidally stable AgNPs-APCG dispersion was prepared by borohydride chemical reduction and contained predominantly spherical metallic AgNPs with an average diameter of 10.4 ± 4.9 nm and a ζ-potential of -52.6 ± 2.4 mV. The obtained dispersion showed pronounced antibacterial activity against the E. amylovora strain, isolated from fire blight-affected shoots, with a MIC of 18.75 mg/L. In the field trial, foliar application at 5 and 25 mg/L reduced fire blight incidence and severity, with biological efficacies of 52.8% and 80.3%, respectively, and increased marketable yield by 12.0% and 16.7%. The phytoprotective efficacy of AgNPs-APCG at 5 mg/L, a concentration nominally below the in vitro MIC for E. amylovora, was consistent with a possible contribution of factors in addition to direct antibacterial activity. Supporting this possibility, AgNPs-APCG reduced carbohydrate-containing exopolysaccharide content in E. amylovora cultures to 51 ± 5% of the untreated control. Furthermore, higher activities of antioxidant enzymes were observed in AgNPs-APCG-treated apple leaves, while malondialdehyde content showed no pronounced increase, suggesting possible modulation of redox- and defense-related metabolism in treated plants. Potential environmental implications of the tested application regimes were considered based on the estimated Ag input to soil. These findings suggest that multiple pathogen- and host-associated responses may contribute to the phytoprotective effect of AgNPs-APCG and support its further evaluation as a multifunctional agent against bacterial plant diseases.
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BibTeX
@article{khina2026silver,
title = {{Silver Nanoparticles Suppress Apple Fire Blight: Antibacterial Activity, Reduced Carbohydrate-Containing EPS Content, and Altered Host Antioxidant Responses.}},
author = {Alexander G Khina and Maria T Mukhina and Alexander S Gordeev and Liliya R Biktasheva and Dmitry M Mikhaylov and Georgii V Lisichkin and Yurii A Krutyakov},
journal = {Plants (Basel, Switzerland)},
year = {2026},
month = oct,
doi = {10.3390/plants15203208},
url = {https://europepmc.org/article/MED/42943161},
note = {CC BY},
}
RIS
TY - JOUR
TI - Silver Nanoparticles Suppress Apple Fire Blight: Antibacterial Activity, Reduced Carbohydrate-Containing EPS Content, and Altered Host Antioxidant Responses.
AU - Alexander G Khina
AU - Maria T Mukhina
AU - Alexander S Gordeev
AU - Liliya R Biktasheva
AU - Dmitry M Mikhaylov
AU - Georgii V Lisichkin
AU - Yurii A Krutyakov
PY - 2026
DA - 2026/10/19
JO - Plants (Basel, Switzerland)
DO - 10.3390/plants15203208
UR - https://europepmc.org/article/MED/42943161
AB - The present study evaluated the phytoprotective activity of silver nanoparticles stabilized with sodium amphopolycarboxyglycinate (AgNPs-APCG) against apple fire blight caused by Erwinia amylovora. A highly colloidally stable AgNPs-APCG dispersion was prepared by borohydride chemical reduction and contained predominantly spherical metallic AgNPs with an average diameter of 10.4 ± 4.9 nm and a ζ-potential of -52.6 ± 2.4 mV. The obtained dispersion showed pronounced antibacterial activity against the E. amylovora strain, isolated from fire blight-affected shoots, with a MIC of 18.75 mg/L. In the field trial, foliar application at 5 and 25 mg/L reduced fire blight incidence and severity, with biological efficacies of 52.8% and 80.3%, respectively, and increased marketable yield by 12.0% and 16.7%. The phytoprotective efficacy of AgNPs-APCG at 5 mg/L, a concentration nominally below the in vitro MIC for E. amylovora, was consistent with a possible contribution of factors in addition to direct antibacterial activity. Supporting this possibility, AgNPs-APCG reduced carbohydrate-containing exopolysaccharide content in E. amylovora cultures to 51 ± 5% of the untreated control. Furthermore, higher activities of antioxidant enzymes were observed in AgNPs-APCG-treated apple leaves, while malondialdehyde content showed no pronounced increase, suggesting possible modulation of redox- and defense-related metabolism in treated plants. Potential environmental implications of the tested application regimes were considered based on the estimated Ag input to soil. These findings suggest that multiple pathogen- and host-associated responses may contribute to the phytoprotective effect of AgNPs-APCG and support its further evaluation as a multifunctional agent against bacterial plant diseases.
SN - 2223-7747
ER -