Cell-specific heteroclinic orbits govern interaction dynamics in inertial microfluidics for circulating tumour cell separation
- 1University of Edinburgh
Abstract
Cancer survival rates increase with earlier diagnosis. However, many cancers are diagnosed only after symptoms develop, in the later stages of cancer progression. We need a diagnostic tool that can detect cancer earlier. Circulating tumour cells (CTCs) are cells that detach from the main tumour and can enter the bloodstream. The capture and analysis of CTCs can provide an early indicator of cancer. However, rarity of CTCs in blood makes their efficient capture challenging. Inertial microfluidics can be utilised for a label-free separation of CTCs from white blood cells (WBCs) by manipulating cells in the microchannel based on cell properties, achieving good separation performance. Inertial microfluidic devices mainly rely on size-based separation. However, residual WBC carryover limits complete separation. Here, we show that cell mechanical heterogeneity plays a large role in the separation process and should be considered in the design of these devices. We simulate the migration dynamics of CTCs and WBCs in a straight microchannel using a 3D lattice-Boltzmann-immersed-boundary-finite-element solver. Our results demonstrate that WBC migration behaviour changes depending on the deformability of a CTC. The size and deformability of the cells have been shown to determine the cell-specific heteroclinic orbits leading to different interaction types, these interactions alter WBC migration resulting in more/less WBC carryover. This work highlights that both cell-specific heteroclinic orbits and single cell migration rates should be considered in the design of inertial microfluidics for separation.
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BibTeX
@misc{hay2026cellspecific,
title = {{Cell-specific heteroclinic orbits govern interaction dynamics in inertial microfluidics for circulating tumour cell separation}},
author = {R. Hay and C. Ghera and J. Zhou and I. Papautsky and T. Krueger and B. Owen},
howpublished = {bioRxiv},
year = {2026},
month = sep,
doi = {10.64898/2026.09.15.751739},
url = {https://www.biorxiv.org/content/10.64898/2026.09.15.751739v1},
note = {CC BY},
}
RIS
TY - UNPB
TI - Cell-specific heteroclinic orbits govern interaction dynamics in inertial microfluidics for circulating tumour cell separation
AU - R. Hay
AU - C. Ghera
AU - J. Zhou
AU - I. Papautsky
AU - T. Krueger
AU - B. Owen
PY - 2026
DA - 2026/09/15
PB - bioRxiv
DO - 10.64898/2026.09.15.751739
UR - https://www.biorxiv.org/content/10.64898/2026.09.15.751739v1
AB - Cancer survival rates increase with earlier diagnosis. However, many cancers are diagnosed only after symptoms develop, in the later stages of cancer progression. We need a diagnostic tool that can detect cancer earlier. Circulating tumour cells (CTCs) are cells that detach from the main tumour and can enter the bloodstream. The capture and analysis of CTCs can provide an early indicator of cancer. However, rarity of CTCs in blood makes their efficient capture challenging. Inertial microfluidics can be utilised for a label-free separation of CTCs from white blood cells (WBCs) by manipulating cells in the microchannel based on cell properties, achieving good separation performance. Inertial microfluidic devices mainly rely on size-based separation. However, residual WBC carryover limits complete separation. Here, we show that cell mechanical heterogeneity plays a large role in the separation process and should be considered in the design of these devices. We simulate the migration dynamics of CTCs and WBCs in a straight microchannel using a 3D lattice-Boltzmann-immersed-boundary-finite-element solver. Our results demonstrate that WBC migration behaviour changes depending on the deformability of a CTC. The size and deformability of the cells have been shown to determine the cell-specific heteroclinic orbits leading to different interaction types, these interactions alter WBC migration resulting in more/less WBC carryover. This work highlights that both cell-specific heteroclinic orbits and single cell migration rates should be considered in the design of inertial microfluidics for separation.
ER -