Novel aptasensor coupling electrochemistry and microfluidics for the detection of luteinising hormone in fertility monitoring
Abstract
The sensitive and real-time detection of Luteinizing Hormone (LH) is essential for monitoring reproductive health and supporting assisted reproductive technologies (ART). In this study, LH was detected within the physiological range using an electrochemical aptasensor integrated into a microfluidic platform. Quantification was achieved via cyclic voltammetry in PBS and 10% diluted horse serum over a concentration range of 0.2 pM to 10 nM. The microfluidic device enabled rapid analysis with a target detection protocol of approximately 30 minutes, making it suitable for near real-time applications. To assess performance in more complex biological matrices, the sensor was also implemented in 20% diluted spiked blood serum and in 25% diluted spiked whole blood with LH. While current responses were decreased due to matrix interference, a clear, concentration-dependent signal was still observed, confirming the sensor's robustness and reliability under given experimental conditions. These results demonstrate the sensor's potential for integration into portable, real-time diagnostic systems for hormone monitoring, particularly in ART and infertility diagnostics.
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BibTeX
@misc{man2026novel,
title = {{Novel aptasensor coupling electrochemistry and microfluidics for the detection of luteinising hormone in fertility monitoring}},
author = {Hiu Mun Man and Marie-Charlotte Horny and Isabelle Le Potier and David Bouville and Téo Baptiste and Anne-Marie Haghiri- Gosnet and Brice Calvignac and Pierre-Emmanuel Bouet and Laurent Thouin and Jean Gamby},
howpublished = {HAL},
year = {2026},
month = sep,
url = {https://hal.science/hal-05772662v1},
note = {CC BY 4.0},
}
RIS
TY - UNPB
TI - Novel aptasensor coupling electrochemistry and microfluidics for the detection of luteinising hormone in fertility monitoring
AU - Hiu Mun Man
AU - Marie-Charlotte Horny
AU - Isabelle Le Potier
AU - David Bouville
AU - Téo Baptiste
AU - Anne-Marie Haghiri- Gosnet
AU - Brice Calvignac
AU - Pierre-Emmanuel Bouet
AU - Laurent Thouin
AU - Jean Gamby
PY - 2026
DA - 2026/09/20
PB - HAL
UR - https://hal.science/hal-05772662v1
AB - The sensitive and real-time detection of Luteinizing Hormone (LH) is essential for monitoring reproductive health and supporting assisted reproductive technologies (ART). In this study, LH was detected within the physiological range using an electrochemical aptasensor integrated into a microfluidic platform. Quantification was achieved via cyclic voltammetry in PBS and 10% diluted horse serum over a concentration range of 0.2 pM to 10 nM. The microfluidic device enabled rapid analysis with a target detection protocol of approximately 30 minutes, making it suitable for near real-time applications. To assess performance in more complex biological matrices, the sensor was also implemented in 20% diluted spiked blood serum and in 25% diluted spiked whole blood with LH. While current responses were decreased due to matrix interference, a clear, concentration-dependent signal was still observed, confirming the sensor's robustness and reliability under given experimental conditions. These results demonstrate the sensor's potential for integration into portable, real-time diagnostic systems for hormone monitoring, particularly in ART and infertility diagnostics.
ER -