Preliminary analysis of the proportions and characteristics of dual TCR T cells and dual BCR B cells in echinococcus granulosus-infected mice using scRNA+TCR+BCR-seq
- 1Department of Urology, Affiliated Hospital of Zunyi Medical University
- 2Department of Immunology, Zunyi Medical University; Guizhou Provincial Research Center for Applied Molecular Immunology; Guizhou Provincial Graduate Education Innovation Base for Immunology; Guizhou Provincial Key Discipline of Immunology
- 3Central Laboratory, Guizhou Aerospace Hospital
Abstract
Introduction: Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by Echinococcus granulosus, and the T and B cell responses and regulatory mechanisms in CE remain unclear.
Methods: In this study, we analyzed the data of peripheral blood and spleen of mice infected with Echinococcus granulosus(EG) by scRNA +TCR+BCR-seq.
Results: We made the following novel findings: (1) Peripheral blood of Echinococcus granulosus (EG)-infected mice contained a higher proportion of dual TCR(T cell receptor) T cells with higher clonality than uninfected mice; (2) There was a bias in V gene usage between single TCR T and dual TCR T cells, as well as between single BCR(B cell receptor) B cells and dual BCR B cells in EG-infected mice; (3) Dual TCR Tregs, dual TCR effector CD8 T cells, dual BCR Bregs, and dual BCR memory/dividing B cells exhibited relatively high clonal expansion in EG-infected mice; (4) Shared CDR3 sequences and consistently highly expressed mRNA molecules exist between single receptor T/B and dual receptor T/B cells in EG-infected mice. KEGG enrichment analysis revealed that dual BCR B cells are closely related to the expression of factors associated with pathways such as "Th1/ Th2 differentiation" and "antigen processing and presentation".
Discussion: These results suggest that dual TCR T and dual BCR B cells may be involved in the specific responses and regulation of EG-infected mice, providing a novel perspective for the adaptive immune response mechanisms and targeted therapy of parasitic infections.
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BibTeX
@article{su2026preliminary,
title = {{Preliminary analysis of the proportions and characteristics of dual TCR T cells and dual BCR B cells in echinococcus granulosus-infected mice using scRNA+TCR+BCR-seq}},
author = {Peng Su and Kai Quan and Hongxia Yang and Yadong Gong and Xinsheng Yao},
journal = {Frontiers in Immunology},
year = {2026},
month = oct,
doi = {10.3389/fimmu.2026.1835604},
url = {https://www.frontiersin.org/articles/10.3389/fimmu.2026.1835604/full},
note = {CC BY 4.0},
}
RIS
TY - JOUR
TI - Preliminary analysis of the proportions and characteristics of dual TCR T cells and dual BCR B cells in echinococcus granulosus-infected mice using scRNA+TCR+BCR-seq
AU - Peng Su
AU - Kai Quan
AU - Hongxia Yang
AU - Yadong Gong
AU - Xinsheng Yao
PY - 2026
DA - 2026/10/13
JO - Frontiers in Immunology
DO - 10.3389/fimmu.2026.1835604
UR - https://www.frontiersin.org/articles/10.3389/fimmu.2026.1835604/full
AB - Introduction: Cystic echinococcosis (CE) is a zoonotic parasitic disease caused by Echinococcus granulosus, and the T and B cell responses and regulatory mechanisms in CE remain unclear. Methods: In this study, we analyzed the data of peripheral blood and spleen of mice infected with Echinococcus granulosus(EG) by scRNA +TCR+BCR-seq. Results: We made the following novel findings: (1) Peripheral blood of Echinococcus granulosus (EG)-infected mice contained a higher proportion of dual TCR(T cell receptor) T cells with higher clonality than uninfected mice; (2) There was a bias in V gene usage between single TCR T and dual TCR T cells, as well as between single BCR(B cell receptor) B cells and dual BCR B cells in EG-infected mice; (3) Dual TCR Tregs, dual TCR effector CD8 T cells, dual BCR Bregs, and dual BCR memory/dividing B cells exhibited relatively high clonal expansion in EG-infected mice; (4) Shared CDR3 sequences and consistently highly expressed mRNA molecules exist between single receptor T/B and dual receptor T/B cells in EG-infected mice. KEGG enrichment analysis revealed that dual BCR B cells are closely related to the expression of factors associated with pathways such as "Th1/ Th2 differentiation" and "antigen processing and presentation". Discussion: These results suggest that dual TCR T and dual BCR B cells may be involved in the specific responses and regulation of EG-infected mice, providing a novel perspective for the adaptive immune response mechanisms and targeted therapy of parasitic infections.
SN - 1664-3224
ER -