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Peer-reviewedCC BY-NC-NDNature Communications

CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies

  • Moises Di Sante1,2
  • Melissa Pezzotti3,2
  • Julius Zimmermann3,2
  • Alessandro Enrico3,2
  • J.R. Deschamps4
  • Elisa Balmas5,6
  • Silvia Becca5,6
  • Eloisa Torchia3,2
  • Giulia Ballio3,6
  • Samantha Solito7,4
  • Martina Sarchi8,2
  • Alessandro Reali3
3 more authors
  • Alessandro Bertero5
  • Florian Jug4
  • Francesco S. Pasqualini9
9 affiliations
  1. 1Synthetic Physiology Laboratory, University of Pavia, Pavia, Italy. [email protected]
  2. 2Synthetic Physiology Laboratory, University of Pavia, Italy
  3. 3Synthetic Physiology Laboratory, University of Pavia, Pavia, Italy
  4. 4Human Technopole, Milan, Italy
  5. 5Molecular Biotechnology Center "Guido Tarone", University of Torino, Torino, Italy
  6. 6Molecular Biotechnology Center Guido Tarone, University of Torino, Italy
  7. 7Centro Grandi Strumenti, University of Pavia, Pavia, Italy
  8. 8Department of Molecular Medicine, University of Pavia, Pavia, Italy
  9. 9Synthetic Physiology Laboratory, University of Pavia, Pavia, Italy. [email protected]
Open at sourceDOICite

Abstract

Cell cycle progression, migration, and proliferation shape development and regeneration, but simultaneous live-cell imaging remains challenging as conventional fluorescent cell cycle indicators (FUCCI) monopolize the green and red channels used by most structural and functional biosensors. To overcome this, we integrated a spectrally re-engineered FUCCI variant, open-source analysis software, and four-color human stem cell reporter lines into CALIPERS: a method for Cell-cycle-Aware Live-cell Imaging in Phenotyping and Regeneration Studies.

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Cite

BibTeXRIS
BibTeX
@article{sante2026calipers,
  title = {{CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies}},
  author = {Moises Di Sante and Melissa Pezzotti and Julius Zimmermann and Alessandro Enrico and J.R. Deschamps and Elisa Balmas and Silvia Becca and Eloisa Torchia and Giulia Ballio and Samantha Solito and Martina Sarchi and Alessandro Reali and Alessandro Bertero and Florian Jug and Francesco S. Pasqualini},
  journal = {Nature Communications},
  year = {2026},
  month = aug,
  doi = {10.1038/s41467-026-76144-9},
  url = {https://doi.org/10.1038/s41467-026-76144-9},
  note = {CC BY-NC-ND},
}
RIS
TY  - JOUR
TI  - CALIPERS: Cell cycle-aware live imaging for phenotyping experiments and regeneration studies
AU  - Moises Di Sante
AU  - Melissa Pezzotti
AU  - Julius Zimmermann
AU  - Alessandro Enrico
AU  - J.R. Deschamps
AU  - Elisa Balmas
AU  - Silvia Becca
AU  - Eloisa Torchia
AU  - Giulia Ballio
AU  - Samantha Solito
AU  - Martina Sarchi
AU  - Alessandro Reali
AU  - Alessandro Bertero
AU  - Florian Jug
AU  - Francesco S. Pasqualini
PY  - 2026
DA  - 2026/08/01
JO  - Nature Communications
DO  - 10.1038/s41467-026-76144-9
UR  - https://doi.org/10.1038/s41467-026-76144-9
AB  - Cell cycle progression, migration, and proliferation shape development and regeneration, but simultaneous live-cell imaging remains challenging as conventional fluorescent cell cycle indicators (FUCCI) monopolize the green and red channels used by most structural and functional biosensors. To overcome this, we integrated a spectrally re-engineered FUCCI variant, open-source analysis software, and four-color human stem cell reporter lines into CALIPERS: a method for Cell-cycle-Aware Live-cell Imaging in Phenotyping and Regeneration Studies.
SN  - 2041-1723
ER  -