Circulating Plasma Adiposomes as a Partial Lipidomic Readout of Matched Subcutaneous Adipose Tissue: Implications for Interorgan Communication
7 affiliations
- 1Division of Endocrinology, Diabetes, and Metabolism, Department of Medicine, College of Medicine University of Illinois Chicago Chicago Illinois USA
- 2Department of Cytology and Histology, Faculty of Veterinary Medicine Suez Canal University Ismailia Egypt
- 3Research Informatics Core University of Illinois Chicago Chicago Illinois USA
- 4Department of Surgery, College of Medicine University of Illinois Chicago Chicago Illinois USA
- 5Jesse Brown Veterans Affairs Medical Center Chicago Illinois USA
- 6Department of Kinesiology and Nutrition, College of Applied Health Sciences University of Illinois Chicago Chicago Illinois USA
- 7Department of Pathology, South Egypt Cancer Institute Assiut University Assiut Egypt
Abstract
Background: Prior studies established adipose‐derived extracellular vesicles, or adiposomes, as mediators of interorgan communication with lipidomic signatures linked to cardiometabolic health. Because adiposomes signal to distant organs via the circulation, we examined whether circulating adiposome lipidomes reflect matched subcutaneous adipose tissue adiposomes.
Methods: This cross‐sectional paired lipidomic study included 22 subjects with matched circulating and subcutaneous adipose tissue adiposome samples. Untargeted lipidomics quantified 773 lipid features across 18 detected broad classes. Spearman correlation was the primary across‐subject species‐level metric; pooled and within‐subject Pearson and Spearman correlations, principal component analysis, Bland–Altman analysis, class composition, and matched‐versus‐mismatched similarity were complementary measures. Additional analyses evaluated sex, cardiometabolic phenotypes, and focused species‐clinical associations.
Results: Across 770 species with valid across‐subject estimates, the median plasma‐subcutaneous Spearman ρ was 0.101 and no species survived Benjamini‐Hochberg false‐discovery‐rate correction, although 553 (71.8%) correlations were positive. Mean broad‐class composition was highly conserved (Spearman ρ = 0.996; Pearson r on log2 percentages = 0.996). Matched whole‐profile similarity was not greater than mismatched similarity (median ρ = 0.818 vs. 0.811; p = 0.620), and the matched sample was the closest pair for 0 of 22 participants. Neither sex nor any prespecified cardiometabolic phenotype explained matched‐profile similarity after correction, and no focused species‐clinical association survived full‐screen FDR correction.
Conclusions: Circulating and subcutaneous adiposome lipidomes share a highly conserved mean class architecture, but individual species, subject‐specific matching, and clinical‐association patterns are not interchangeable. Plasma adiposomes may therefore serve as complementary circulating readouts of adipose‐derived interorgan lipid signaling rather than direct tissue substitutes.
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BibTeX
@article{rakab2026circulating,
title = {{Circulating Plasma Adiposomes as a Partial Lipidomic Readout of Matched Subcutaneous Adipose Tissue: Implications for Interorgan Communication}},
author = {Mohamed Saad Rakab and Imaduddin Mirza and Elsayed Metwally and Mohammed Morsy and Carlos Martinez and Mario Masrur and Chandra Hassan and Francesco M. Bianco and Brian T. Layden and Abeer M. Mahmoud},
journal = {Comprehensive physiology},
year = {2026},
month = nov,
doi = {10.1002/cph4.70296},
url = {https://europepmc.org/article/PMC/PMC13724754},
note = {CC BY},
}
RIS
TY - JOUR
TI - Circulating Plasma Adiposomes as a Partial Lipidomic Readout of Matched Subcutaneous Adipose Tissue: Implications for Interorgan Communication
AU - Mohamed Saad Rakab
AU - Imaduddin Mirza
AU - Elsayed Metwally
AU - Mohammed Morsy
AU - Carlos Martinez
AU - Mario Masrur
AU - Chandra Hassan
AU - Francesco M. Bianco
AU - Brian T. Layden
AU - Abeer M. Mahmoud
PY - 2026
DA - 2026/11/01
JO - Comprehensive physiology
DO - 10.1002/cph4.70296
UR - https://europepmc.org/article/PMC/PMC13724754
AB - Background: Prior studies established adipose‐derived extracellular vesicles, or adiposomes, as mediators of interorgan communication with lipidomic signatures linked to cardiometabolic health. Because adiposomes signal to distant organs via the circulation, we examined whether circulating adiposome lipidomes reflect matched subcutaneous adipose tissue adiposomes. Methods: This cross‐sectional paired lipidomic study included 22 subjects with matched circulating and subcutaneous adipose tissue adiposome samples. Untargeted lipidomics quantified 773 lipid features across 18 detected broad classes. Spearman correlation was the primary across‐subject species‐level metric; pooled and within‐subject Pearson and Spearman correlations, principal component analysis, Bland–Altman analysis, class composition, and matched‐versus‐mismatched similarity were complementary measures. Additional analyses evaluated sex, cardiometabolic phenotypes, and focused species‐clinical associations. Results: Across 770 species with valid across‐subject estimates, the median plasma‐subcutaneous Spearman ρ was 0.101 and no species survived Benjamini‐Hochberg false‐discovery‐rate correction, although 553 (71.8%) correlations were positive. Mean broad‐class composition was highly conserved (Spearman ρ = 0.996; Pearson r on log2 percentages = 0.996). Matched whole‐profile similarity was not greater than mismatched similarity (median ρ = 0.818 vs. 0.811; p = 0.620), and the matched sample was the closest pair for 0 of 22 participants. Neither sex nor any prespecified cardiometabolic phenotype explained matched‐profile similarity after correction, and no focused species‐clinical association survived full‐screen FDR correction. Conclusions: Circulating and subcutaneous adiposome lipidomes share a highly conserved mean class architecture, but individual species, subject‐specific matching, and clinical‐association patterns are not interchangeable. Plasma adiposomes may therefore serve as complementary circulating readouts of adipose‐derived interorgan lipid signaling rather than direct tissue substitutes.
SN - 2040-4603
ER -